Assessment of Iontophoretic and Passive Ungual Penetration by Laser Scanning Confocal Microscopy

Dutet, Julie; Delgado‐Charro, M. Begoña

doi:10.1007/s11095-012-0841-2

Cited by 12 publications

(10 citation statements)

References 39 publications

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“…These domains may therefore provide a sympathetic environment for small, hydrophobic molecules and constitute a potential lipophilic pathway for nail permeation. However, it should be noted that recent work (16) investigated the iontophoretic penetration of fluorescent markers, sodium fluorescein and Nile blue chloride, into the nail and the results supported a transcellular pathway. The apparent difference observed in the current study may suggest different penetration pathways for iontophoretic and passive transport.…”

Section: Lscm Imaging In Vitro Uptake Of Nr Into Non-porated Nailsmentioning

confidence: 89%

Drug delivery into microneedle-porated nails from nanoparticle reservoirs

Chiu

Belsey

Garrett

et al. 2015

Journal of Controlled Release

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This study demonstrates the potential of polymeric nanoparticles as drug reservoirs for sustained topical drug delivery into microneedle-treated human nail. Laser scanning confocal microscopy was used to image the delivery of a fluorescent model compound from nanoparticles into the nail. A label-free imaging technique, stimulated Raman scattering microscopy, was applied, in conjunction with two-photon fluorescence imaging, to probe the disposition of nanoparticles and an associated lipophilic 'active' in a microneedle-porated nail. The results provide clear evidence that the nanoparticles function as immobile reservoirs, sequestered on the nail surface and in the microneedle-generated pores, from which the active payload can be released and diffuse laterally into the nail over an extended period of time.

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Section: Lscm Imaging In Vitro Uptake Of Nr Into Non-porated Nailsmentioning

confidence: 89%

Drug delivery into microneedle-porated nails from nanoparticle reservoirs

Chiu

Belsey

Garrett

et al. 2015

Journal of Controlled Release

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“…Despite the difference in the physicochemical properties of both the markers, NBC (353.85 Da, logP = 4.99) and SF (376.28 Da, logP = À0.67), depth of penetration of both the compounds was found to be similar under passive as well as iontophoretic conditions. The even distribution of the compound and the confocal images suggested the transcellular pathway to be predominant during both passive and iontophoretic experiments [27].…”

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 89%

“…Dutet et al [27] investigated the depth, uniformity and pathways of penetration of two fluorescent markers (nile blue chloride, NBC; and sodium fluorescein, SF) into human nail during passive and iontophoretic experiment using CLSM. The fluorescent markers were found to be penetrated 7-12% of the nail thickness.…”

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

Structural and component mining of nails using bioengineering techniques

Thatai

Sapra

2016

Intern J of Cosmetic Sci

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The human nail is one of the challenging membranes for the scientists to target and to improve the clinical efficacy of ungual formulations. The understanding of nail physiology, impact of hydration on its properties and presence of trace elements in nails as biomarkers has been explored by various researchers in clinical studies. Despite the importance of biophysical techniques for the assessment of structure and physiology of nail, minimum literature analyses biophysical, biochemical and bioanalytical approaches. However, nowadays scientists in bioengineering field are keen in developing non-invasive, reliable and reproducible techniques for the assessment of different anatomical and functional parameters of nails for testing of ungual products.

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“…Here, we summarize the literature on the use of confocal microscopy for the study of healthy and pathological nail . The review was performed utilizing PubMed database.…”

Section: Introductionmentioning

confidence: 99%

“…Here, we summarize the literature on the use of confocal microscopy for the study of healthy and pathological nail. [5][6][7][8][9][10][11][12][13][14] The review was performed utilizing PubMed database. Search terms employed were 'nail', 'reflectance confocal microscopy', 'confocal microscopy' and 'onychomycosis'.…”

Section: Introductionmentioning

confidence: 99%

Confocal microscopy for healthy and pathological nail

Cinotti

Fouilloux

Perrot

et al. 2013

Acad Dermatol Venereol

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Nail diseases are often annoying for the patient and diagnostically challenging for dermatologists. New imaging techniques are of high interest in the diagnosis of nail disorders to reduce the number of nail biopsies. Confocal microscopy is a high-resolution emerging imaging technique that can be used to explore the entire body surface, including skin, mucosa, hair and nails. A systematic review of the literature concerning the use of confocal microscopy for the study of either healthy or pathological nail has been performed to evaluate the current use of this technique and possible future applications. Confocal microscopy is particularly suitable for nails because it allows a non-invasive in vivo examination of this sensitive body area, and nail plate transparency permits to image up to the nail bed with an easy identification of corneocytes. Confocal microscopy can play a role in the diagnosis of onychomycosis and melanonichia, and in the study of drug penetration through the nail plate. It could be used in the future as a non-invasive procedure for the investigation of different nail diseases, such as psoriasis and lichen planus. Further application could be the intra-operative ex vivo examination of nail specimens to outline tumour margins to assist surgery.

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Assessment of Iontophoretic and Passive Ungual Penetration by Laser Scanning Confocal Microscopy

Abstract: Iontophoresis enhanced moderately the penetration of the two markers into the nail plate as compared to passive diffusion. The confocal images suggested the transcellular pathway to be predominant during both passive and iontophoretic experiments.

Cited by 12 publications

References 39 publications

Drug delivery into microneedle-porated nails from nanoparticle reservoirs

Drug delivery into microneedle-porated nails from nanoparticle reservoirs

Structural and component mining of nails using bioengineering techniques

Confocal microscopy for healthy and pathological nail

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