Assessment of interactions between glutamine and glucose on meat quality, AMPK, and glutamine concentrations in pectoralis major meat of broilers under acute heat stress

Hu, Hong; Bai, Xi; Wen, Aiyou; Shah, Assar Ali; Dai, Sifa; Ren, Q. C.; Wang, S.; He, Shang; Wang, L.

doi:10.3382/japr/pfw021

Cited by 13 publications

(12 citation statements)

References 19 publications

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“…One possible explanation for the above beneficial effects is that nucleotide supplementation may conserve amino acids such as glutamine, aspartate, and glycine for de novo synthesis, resulting in the use of these amino acids for growth. Increased glutamine availability is particularly important for high-glutamine-demand processes and organs such as the pectoralis major muscle (0.06 g glutamine/g protein of muscle; Hu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary nucleotide supplementation on growth performance and physiology of broiler chickens under pre- and post-inflammatory challenge

Kreuz¹,

Rocha²,

Campos³

et al. 2020

Revista Brasileira De Zootecnia

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The objective of this study was to evaluate the effects of dietary nucleotide supplementation on growth performance, serum immunoglobulin G (IgG) and uric acid levels, immune-related organs, and intestinal morphometric parameters of broiler chickens under pre-and post-inflammatory challenge with lipopolysaccharide (LPS). Ninety-six seven-day-old male broiler chicks were randomized in a 2×2 factorial design composed of two dietary types (supplemented with 0 and 0.3 g of purified nucleotides/kg of feed) and two inflammatory conditions (with and without LPS administration) with eight replicates per treatment and three birds per cage. The experimental period was divided into two phases: 7-20 days of age (pre-LPS challenge) and 21-35 days of age (post-LPS challenge). Data were analyzed using one-way and two-way ANOVA for the pre-and post-challenge phases, respectively. During the prechallenge phase, dietary nucleotide supplementation improved body weight (982 vs. 1009 g/bird), daily weight gain (58.1 vs. 60.1 g/bird), feed conversion rate (1.28 vs. 1.25 g/g), and serum concentration of IgG (1.17 vs. 1.76 mg/mL) of broilers. During the post-challenge phase, nucleotide supplementation increased serum IgG of broilers at 28 days of age (3.01 vs. 4.34 mg/mL). For the intestinal morphometric parameters, nucleotide supplementation increased villus height (299.3 vs. 315.6 μm). Dietary nucleotide supplementation improves the performance of broilers from seven to 20 days of age (pre-LPS challenge). However, from 21 to 35 days of age, supplementation of 0.3 g of purified nucleotides/kg of feed, independent of inflammatory challenge, does not contribute to improve performance of broilers. Additionally, nucleotide supplementation increases IgG production and villus height in the jejunum of broilers.

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Section: Discussionmentioning

confidence: 99%

Effects of dietary nucleotide supplementation on growth performance and physiology of broiler chickens under pre- and post-inflammatory challenge

Kreuz¹,

Rocha²,

Campos³

et al. 2020

Revista Brasileira De Zootecnia

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“…Meat quality was determined 45 min after euthanasia. The pH value of the thigh muscle was measured using a pH meter (Mettler Toledo, Zurich, Switzerland) as per the procedure described by Hu et al. (2016a) .…”

Section: Methodsmentioning

confidence: 99%

“…(2016a) . The luminance (L*), redness (a*), and yellowness (b*) of the thigh muscle were measured using a colorimeter (Minolta, Tokyo, Japan) as described by Hu et al. (2016a) .…”

Section: Methodsmentioning

confidence: 99%

“…Gln is well known to have positive effects on heat-stressed broilers ( Shakeri et al., 2014 , Wu et al., 2018 ). Hu et al., 2016a , Jazideh et al., 2014 , and Olubodun et al. (2015) suggested that dietary Gln improves the performance, carcass characteristics, serum parameters, intestinal immunity, and meat quality of broilers under heat stress.…”

Section: Introductionmentioning

confidence: 99%

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Glutamine improves heat stress–induced oxidative damage in the broiler thigh muscle by activating the nuclear factor erythroid 2–related 2/Kelch-like ECH-associated protein 1 signaling pathway

Dai

et al. 2020

Poultry Science

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The aim of the present study was to evaluate the effect of glutamine ( Gln ) on modulating heat stress–induced oxidative damage in the broiler thigh muscle through nuclear factor erythroid 2–related 2/Kelch-like ECH-associated protein 1 ( Nrf2-Keap1 ) pathway. Three-hundred 22-day-old Arbor Acres broilers were reallocated into 5 groups: a control group (24 °C) fed with basal diet and 4 heat stress ( HS ) groups (34 °C for 8 h/D) fed with basal diet containing 0, 0.5, 1.0, and 1.5% Gln. This experiment lasted 21 D. Heat stress decreased ( P < 0.05) pH, redness, and Gln levels, and increased ( P < 0.05) luminance, water loss rate, and cooking loss ( CL ) values of the thigh meat. Compared with the HS group, supplementation with 1.5% Gln increased ( P < 0.05) pH, redness, and Gln levels, but decreased ( P < 0.05) luminance and CL values in the thigh meat. There were significant decreases ( P < 0.05) in glutathione ( GSH ), superoxide dismutase ( SOD ), catalase ( CAT ), glutathione peroxidase ( GSH-Px ), total antioxidant capacity ( T-AOC ), and Nrf2 levels, but significant increases ( P < 0.05) in the malondialdehyde ( MDA ) and Keap1 levels of the thigh muscle after HS treatment. Compared with the HS group, supplementation with 1.0, and 1.5% Gln decreased ( P < 0.05) MDA and Keap1 levels; supplementation with 1.5% Gln increased ( P < 0.05) GSH, GSH-Px, T-AOC, CAT, SOD, and Nrf2 levels in the thigh muscle of heat-stressed broilers. Furthermore, HS decreased ( P < 0.05) Nrf2, SOD, CAT, and GSH-Px mRNA expression levels, but increased ( P < 0.05) Keap1 mRNA level in the thigh muscle of broiler. Dietary supplementation with 1.5% Gln increased ( P < 0.05) Nrf2, GSH-Px, CAT, and SOD mRNA expression levels, but decreased ( P < 0.05) Keap1 mRNA level in the thigh muscle of heat-stressed broilers. In conclusion, dietary Gln improved the resistance of heat-stressed broiler muscles to oxidative damage possibly through reversing the muscle Gln level and inducing the expression of the Nrf2-Keap1 pathway.

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“…The incidence of PSE could be increased by heat stress (exposure to high ambient temperature) in birds [20,21], probably due to the altered metabolism, acid/base status, oxidative reactions, and changes in hormonal secretions [22]. Although several studies have examined the in uence of acute heat stress ranging from 32 °C to 41 °C on chicken breast quality [21,[23][24][25][26][27][28][29], inconsistent results were observed when the antemortem acute heat stress ranged from 36 °C to 41 °C [21,25,28,29]. Meat quality, such as cooking loss, color attributes, and water holding capacity in chicken breast were impaired by one-hour antemortem acute heat stress at 36 °C and 37 °C [21,28] but not at 40 °C and 41 °C [25,29].…”

mentioning

confidence: 99%

The Increase of Short-term Heat Exposure Temperature at Extreme Range Decreased Phosphofructokinase-1 Activity and AMPK Phosphorylation in Postmortem Chicken Breast Muscle

Zhang

Zhai

Luo

et al. 2020

Preprint

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Preslaughter exposure to high ambient temperature could accelerate postmortem glycolysis and impair chicken breast (pectoralis major muscle) quality. However, previous studies indicated that it might be different when the temperature of short-term heat exposure (SHE) raises to extreme range (above 38°C). Therefore, the objectives of this study were to evaluate the effect of SHE in the range of extreme temperatures (36°C, 38°C, and 40°C) on the activity of glycolytic enzymes and AMP-activated protein kinase (AMPK) in postmortem muscles. The activity of key glycolytic enzymes and phosphorylation of Threonine 172 in AMPK α subunit (p-AMPK-α[Thr172]) during early postmortem (0.1, 1, 2, and 4 h) and the muscle pH decline (0.1, 1, 2, 4, and 24 h) were evaluated. SHE did not affect (P > 0.05) glycogen phosphorylase a (GPa) and pyruvate kinase (PK) activity within 4 h postmortem. However, the phosphofructokinase-1 (PFK-1) activity and phosphorylation of AMPK-α[Thr172] decreased (P < 0.05) as antemortem SHE temperature increased from 36°C to 40°C, which could explain the faster pH decline in the 36°C group at 1 h and 2 h postmortem (P < 0.05), and it could be attributed to proteotoxic stress response resulting from extreme heat exposure. Hexokinase (HK) activity was also affected by the SHE temperature (P < 0.05) with the 36°C group having the highest (P < 0.05) activity within 4 h postmortem. The results of the current study, for the first time, indicated that the increase of antemortem SHE temperature at extreme range does not always accelerate the pH decline rate and increase postmortem glycolytic enzyme activity and AMP-activated protein kinase phosphorylation and that the postmortem metabolism is dependent on SHE temperature. Further research is necessary to explore the biochemical basis of this temperature-dependent pH decline, glycolytic enzyme activity, and AMPK phosphorylation.

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Assessment of interactions between glutamine and glucose on meat quality, AMPK, and glutamine concentrations in pectoralis major meat of broilers under acute heat stress

Cited by 13 publications

References 19 publications

Effects of dietary nucleotide supplementation on growth performance and physiology of broiler chickens under pre- and post-inflammatory challenge

Effects of dietary nucleotide supplementation on growth performance and physiology of broiler chickens under pre- and post-inflammatory challenge

Glutamine improves heat stress–induced oxidative damage in the broiler thigh muscle by activating the nuclear factor erythroid 2–related 2/Kelch-like ECH-associated protein 1 signaling pathway

The Increase of Short-term Heat Exposure Temperature at Extreme Range Decreased Phosphofructokinase-1 Activity and AMPK Phosphorylation in Postmortem Chicken Breast Muscle

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