Assessment of inoculation methods to identify resistance to Fusarium crown rot in wheat

Erginbaş-Orakci, Gül; Poole, Grant J.; Nıcol, J. M.; Paulitz, T. C.; Dababat, Abdelfattah A.; Campbell, K. G.

doi:10.1007/s41348-016-0001-8

Cited by 23 publications

(16 citation statements)

References 26 publications

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“…The use of resistant cultivars is one of the most effective strategies for reducing CR damage, but requires rapid, reliable, and reproducible CR screening assays. Various high-throughput screening methods have been used to identify novel sources of CR resistance in wheat [ 29 , 34 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ], and a previous study investigated three different inoculation techniques (seedling dip, stem base droplet, and colonized grain) as potential methods [ 43 ]. The colonized grain method gave the best results, thus this method was also applied in the current study to minimize the variation often reported in CR screening tests.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat

Erginbaş-Orakci¹,

Sehgal

Sohail

et al. 2018

IJMS

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Crown rot (CR), caused by various Fusarium species, is a major disease in many cereal-growing regions worldwide. Fusarium culmorum is one of the most important species, which can cause significant yield losses in wheat. A set of 126 advanced International Maize and Wheat Improvement Center (CIMMYT) spring bread wheat lines were phenotyped against CR for field crown, greenhouse crown and stem, and growth room crown resistance scores. Of these, 107 lines were genotyped using Diversity Array Technology (DArT) markers to identify quantitative trait loci linked to CR resistance by genome-wide association study. Results of the population structure analysis grouped the accessions into three sub-groups. Genome wide linkage disequilibrium was large and declined on average within 20 cM (centi-Morgan) in the panel. General linear model (GLM), mixed linear model (MLM), and naïve models were tested for each CR score and the best model was selected based on quarantine-quarantine plots. Three marker-trait associations (MTAs) were identified linked to CR resistance; two of these on chromosome 3B were associated with field crown scores, each explaining 11.4% of the phenotypic variation and the third MTA on chromosome 2D was associated with greenhouse stem score and explained 11.6% of the phenotypic variation. Together, these newly identified loci provide opportunity for wheat breeders to exploit in enhancing CR resistance via marker-assisted selection or deployment in genomic selection in wheat breeding programs.

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Section: Discussionmentioning

confidence: 99%

Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat

Erginbaş-Orakci¹,

Sehgal

Sohail

et al. 2018

IJMS

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“…A recent study compared three inoculation methods for their suitability in germplasm evaluation (Erginbas‐Orakci et al ., ). The seedling dip method originally developed by Li et al .…”

Section: Breeding For Fcr Resistance and Tolerancementioning

confidence: 97%

“…() produced the most severe infections, followed by the colonized grain (Nelson and Burgess, ; Wildermuth and McNamara ) and droplet inoculation (Mitter et al ., ) methods. However, inoculations using the colonized grains produced results that were more consistent with the known field rankings of the tested wheat cultivars (Erginbas‐Orakci et al ., ). It should be noted that the use of different field ranking procedures by different workers increases the challenge of finding glasshouse inoculation procedures that correlate with field rankings.…”

Section: Breeding For Fcr Resistance and Tolerancementioning

confidence: 97%

“…A recent study compared three inoculation methods for their suitability in germplasm evaluation (Erginbas-Orakci et al, 2016). The seedling dip method originally developed by Li et al (2008) produced the most severe infections, followed by the colonized grain (Nelson and Burgess, 1994;Wildermuth and McNamara 1994) and droplet inoculation methods.…”

Section: Assessment Of Fcr Resistance and Tolerancementioning

confidence: 99%

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Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects

Kazan

Gardiner

2018

Molecular Plant Pathology

203

158

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Diseases caused by Fusarium pathogens inflict major yield and quality losses on many economically important plant species worldwide, including cereals. Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, is a cereal disease that occurs in many arid and semi-arid cropping regions of the world. In recent years, this disease has become more prevalent, in part as a result of the adoption of moisture-preserving cultural practices, such as minimum tillage and stubble retention. In this pathogen profile, we present a brief overview of recent research efforts that have not only advanced our understanding of the interactions between F. pseudograminearum and cereal hosts, but have also provided new disease management options. For instance, significant progress has been made in the genetic characterization of pathogen populations, the development of new tools for disease prediction, and the identification and pyramiding of loci that confer quantitative resistance to FCR in wheat and barley. In addition, transcriptome analyses have revealed new insights into the processes involved in host defence. Significant progress has also been made in understanding the mechanistic details of the F. pseudograminearum infection process. The sequencing and comparative analyses of the F. pseudograminearum genome have revealed novel virulence factors, possibly acquired through horizontal gene transfer. In addition, a conserved pathogen gene cluster involved in the degradation of wheat defence compounds has been identified, and a role for the trichothecene toxin deoxynivalenol (DON) in pathogen virulence has been reported. Overall, a better understanding of cereal host-F. pseudograminearum interactions will lead to the development of new control options for this increasingly important disease problem. Taxonomy: Fusarium pseudograminearum O'Donnell & Aoki; Kingdom Fungi; Phylum Ascomycota; Subphylum Pezizomycotina; Class Sordariomycetes; Subclass Hypocreomycetidae; Order Hypocreales; Family Nectriaceae; Genus Fusarium. Disease symptoms: Fusarium crown rot caused by F. pseudograminearum is also known as crown rot, foot rot and root rot. Infected seedlings can die before or after emergence. If infected seedlings survive, typical disease symptoms are browning of the coleoptile, subcrown internode, lower leaf sheaths and adjacent stems and nodal tissues; this browning can become evident within a few weeks after planting or throughout plant development. Infected plants may develop white heads with no or shrivelled grains. Disease symptoms are exacerbated under water limitation. Identification and detection: Fusarium pseudograminearum macroconidia usually contain three to five septa (22-60.5 × 2.5-5.5 μm). On potato dextrose agar (PDA), aerial mycelia appear floccose and reddish white, with red or reddish-brown reverse pigmentation. Diagnostic polymerase chain reaction (PCR) tests based on the amplification of the gene encoding translation elongation factor-1a (TEF-1a) have been developed for molecular identification. Host range: All maj...

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“…Plants were harvested and assessed based on the browning/rotting percentage on the crown which describes the stem (1 cm above soil level) according to the modified 1-5 scale: 1= 1-9% Resistant (R), 2= 10-29% Moderately Resistant (MR), 3= 30-69% Moderately Susceptible (MS), 4= 70-89% Susceptible (S), 5= 90-100% Highly Susceptible (HS) (Wildermuth and McNamara 1994;Erginbas-Orakci et al, 2016). The data were analyzed according to standart analysis of variance.…”

Section: Resultsmentioning

confidence: 99%

Determination of Resistance in Winter Wheat Genotypes to the Dryland Root Rots caused by Fusarium culmorum in Turkey

Orakci

Morgounov

Dababat

2018

Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi

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The dryland root rot (foot/crown) caused by Fusarium spp. attacks cereals especially wheat and causes severe yield loss by reducing both grain quantity and quality. Among those Fusarium species attacking wheat crop is the Fusarium culmorum species which has been reported as the main crown rot causal agent in Turkey. Unfortunately, up-to-date, there is only some wheat genotypes with partial resistant to Fusarium spp. Therefore, this study was carried out to find new sources of resistance in diverse wheat genotypes to limit the damage caused by Fusarium disease. In this study, a total of 141 genotypes and breeding lines were obtained from 19 different countries, provided via the International Winter Wheat Improvement Program (IWWIP) and screened for their resistance reactions to a local isolate of Fusarium culmorum under three different environmental conditions (growth room, greenhouse and field) in Turkey in 2012. The best performed genotypes in terms of resistant were then rescreened in 2013 for data validation. Out of the 141 phenotyped wheat genotypes, 17 genotypes (12 %) ranked as moderately resistant (MR) at seedling and/or adult growth stage. The genotypes from Mexico seemed to have adult plant resistant rather than seedling resistance which was higher in the USA genotypes. Winter bread wheats PATWIN YR5

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Assessment of inoculation methods to identify resistance to Fusarium crown rot in wheat

Cited by 23 publications

References 26 publications

Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat

Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat

Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects

Determination of Resistance in Winter Wheat Genotypes to the Dryland Root Rots caused by Fusarium culmorum in Turkey

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