Assessment of indicators and collection methodology to estimate nutrient digestibility in buffaloes

Abstract

-Dry fecal matter production was estimated from neutral detergent indicators on indigestible fiber, indigestible acid detergent fiber, indigestible dry matter, incubated for 144 hours and 288 hours, as well as chromium oxide (Cr 2 O 3 ) and enriched and purified isolated lignin (LIPE ® ) in two sampling schemes (3 and 5 days) on buffaloes. Sample consisted of five castrated animals with average weight of 300 ± 0.6 kg fed on elephant grass cv Cameroon (Pennisetum purpureum).Experimental design consisted of randomized blocks in subdivided plots. Production of dry fecal matter was overestimated when using Cr 2 O 3 , indigestible acid detergent fiber 144 hours, indigestible neutral detergent fiber 144 hours, indigestible neutral detergent fiber 288 hours and indigestible dry matter 144 hours, while indigestible acid detergent fiber 288 hours, indigestible dry matter 288 hours and LIPE ® did not differ from total collection. The same result was observed for apparent digestibility of nutrients. There was no difference in dry fecal matter production and digestibility between both collection periods of 3 and 5 days, demonstrating that a collection period of three days can be used to estimate dry fecal matter production in buffaloes.A three-day period of sample collection, in order to estimate dry fecal matter production and apparent digestibility coefficients, is therefore recommended. The use of LIPE ® , fibers in indigestible acid detergent and indigestible dry matter as indicators, both latter incubated for 288 hours, result in accurate estimates of dry fecal matter production in confined buffaloes, fed on a forage based diet.

Effects of naturally-produced lovastatin on feed digestibility, rumen fermentation, microbiota and methane emissions in goats over a 12-week treatment period

“…Each CH 4 measurement consisted of 5 goats, randomly selected from each treatment groups, was carried out for two consecutive days, making up to a total of 8 days per feeding period (5 goats per two days) to complete the measurement for the 20 animals. Before the CH 4 measurements, animals were transferred to metabolic crates for a five-day apparent total-tract digestibility trial using the total fecal collection procedure [ 18 ]. After the daily total fecal outputs were determined, 10% of the daily feces from each animal was sampled and stored and later pooled by animal at the end of the 5 days digestibility trial, dried in a forced-air oven at 60°C for 48 hours, and ground through a 2mm sieve before being subjected to further analysis.…”

Internal Indicators of Digestibility in Diets Containing Ammoniated Hay from Leaves of the Babassu Palm in the Maintenance of Goats

“…The DMi, NDFi and ADFi indicators were incubated in the rumen for 264 h and proved to be efficient in predicting faecal DM excretion and DM digestibility. Therefore, obtaining indicators for total diets containing hay from leaves of the babassu palm required less incubation time than the 288 h required to predict faecal DM excretion using the DMi and ADFi indicators in buffalo fed elephant grass 'Cameroon' (Pennisetum purpureum) (Soares et al, 2011).…”

Tempo de coleta para determinação dos coeficientes de digestibilidade de nutrientes em equinos

“…Nos últimos anos o indicador LIPE ® (lignina purificada e enriquecida) vem sendo utilizado para auxiliar na determinação dos coeficientes de digestibilidade de nutrientes em equinos (Moss et al, 2017;Figueiredo et al, 2019), e quando comparado com o marcador externo óxido crômico, demonstrou não haver diferenças entre os indicadores (Lazentta et al, 2009), possibilitando assim, seu uso na determinação de coeficientes de digestibilidade para equinos. Além disso, o uso da LIPE® permite reduzir o período de amostragem, obtendo resultados confiáveis, menor manipulação das fezes e otimização do tempo, pois a coleta é realizada uma vez ao dia (Soares et al, 2011). Neste método, a amostra não é destruída após a análise para sua dosagem na espectroscopia no infravermelho (Saliba, 2005), o que permite manter a integridade da amostra para análises posteriores, se necessário.…”

“…Assim, a precisão dos indicadores em determinar a digestibilidade dos nutrientes da dieta pode influenciar na escolha das metodologias e, consequentemente, mudar as estimativas de produção fecal, consumo e digestibilidade (Soares et al, 2011).…”