Assessment of In Vitro Antioxidant and Anti-Inflammatory Potentials of Methanol Extract of Chrysophyllum albidum Cotyledon

Abstract

Background: This study was aimed at analysing the phytochemicals in Chrysophyllum albidum cotyledon extract and their in vitro antioxidant and anti-inflammatory effects. Methods: The Chrysophyllum albidum cotyledon methanol extract (CCME) was phytochemically screened, and flavonoids and phenol contents, antioxidant and anti-inflammatory assays were carried out on the extract using standard procedures. Results: Phytochemicals analysis revealed the presence of steroids, tannins, flavonoid, saponin, triterpenes and xanthoproteins. The phenolic concentration, total flavonoids concentration and sugar concentration were found to be 26.720.048 μg of Tannic Acid Equivalent (TAE /mg, 23.121.92 μg of Rutin Equivalent (RTE)/mg (10.491.12 μg of Quercetin Equivalent (QE)/mg) and 778.3812.82 μg of glucose/ml respectively. The extract demonstrated significant (P<0.05) inhibitory effect compared with the standards as potent antioxidant with DPPH IC50 of 550.52 ± 82.83 μg/ml, lipid peroxidation (45.85 % – 65.85 %), Ferric reducing power showed linear correlation to the standard and the anti-inflammatory potential with (22.06 % – 26.37 %) percentage protection of the human red blood membrane stability and the percentage inhibition of denaturation of albumin (3.42 % – 7.32 %). Conclusion: The study showed that C. albidum cotyledon methanol extract is a potent antioxidant and anti-inflammatory agent to oxidative stress and pathological disease caused by reactive species.