“…While a few polypeptides, including the dominant 62-to 64-kDa polypeptide, demonstrated seroreactivity in neutral and positively charged liposomes, a larger number were seroreactive in association with negatively charged vesicles, with antisera obtained through the corre- (37), and its role in induction of protection against murine cutaneous leishmaniasis has been extensively investigated (25,51,64). The subjects of the studies range from the use of the native protein (13,16,51) to the use of recombinant gp63 (rgp63) expressed in Salmonella spp. (64) or plasmid pCMV, which encodes gp63 (62), to the use of T-cell epitopes within gp63 (25,63).…”