Assessment of Annona reticulata Linn. leaves fractions for invitro antioxidative effect and antimicrobial potential against standard human pathogenic strains

Abstract

Since from long time the plant, Annona reticulata Linn. is known for its beneficial effects. Leaves of A. reticulata were screened for phytochemicals and in vitro antioxidant, antibacterial and antifungal activity. The shade dried leaves were extracted with methanol and aqueous methanolic extract was partitioned successively with n-butanol, chloroform and acetone solvents. Methanolic extract was subjected to antioxidant screening using DPPH free radical scavenging activity and H 2 O 2 scavenging activity. Antibacterial and antifungal activity of extract and fractions were analyzed on eight different clinical bacterial and fungal strains using agar well diffusion method and broth dilution method (MIC and MMC determination). The antioxidant activity showed that the extracts exhibited scavenging effect in concentration-dependent manner. The extract showed potent inhibitory effect against Bacillus subtilis and Escherichia coli bacterial strains while in case of fungal strains the maximum effect was observed against Candida blanki. The maximum zone of inhibition of n-butanol, chloroform and acetone fractions was observed against B. subtilis, and E. coli respectively while all fractions exhibited potent inhibitory effect against C. blanki. MIC and MBC values were determined for active samples, methanol extract and chloroform fraction against Staphylococcus aureus, B. subtilis, E. coli and Pseudomonas aeruginosa which revealed lower MIC and MBC values. The fungal strains Candida albicans, Saccharomyces cerevisiae and C. blanki were used to calculate MIC and MFC values for methanol extract and acetone fraction which This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Please cite this article in press as: Jamkhande PG et al. Assessment of Annona reticulata Linn. leaves fractions for invitro antioxidative effect and antimicrobial potential against standard human pathogenic strains, Alex J Med (2015), http://dx.doi.org/10.1016/j.ajme.2014.12.007demonstrated lower MIC and MFC values. The results provided evidence that the plant is richly supplied with numerous phytoconstituents that might indeed be potential sources of natural antioxidant, antimicrobial agents and supplementary food.

Antioxidant and GC–MS analysis of Annona reticulata leaves extract against unsecure free radicals

“…Anti-proliferative activities of A. reticulata were documented in pharmacological studies [11]. Few studies were carried out on the antioxidant activity of A. reticulata using single organic solvent extract [12][13][14][15], but lacks of executing the complete antioxidative profile of A. reticulata leaves extract using various solvent system. Therefore, in the present study is aimed to investigate the complete screening and evaluation of free radical scavenging activity from the leaves extracts' of A. reticulata using various solvent system, and quantifying antioxidant compounds of phenolic and flavanoid contents in each solvent extracts.…”

In vitro evaluation of Anticancer Activity of Methnaolic Extract of Annona reticulata Linn. (Ramphal) Leaves on Different Human Cancer Cell Lines

“…Plants of Annonaceous family are rich in acetogenins, an anticancer property phytochemical [6]. The presence of acetogenin may be attributed to anticancer effect of methanolic extract of Annona reticulata Linn.…”

“…Different plant parts are effectively employed as folk medicine in the rural area of India. The plant is rich in several phytochemicals such as dopamine, salsolinol, coclaurine, sesquiterpenes and acetogenin [6]. It also contains minerals such as Ca, P, K, Mg, Na, Cl, S, Mn, Zn, Fe, Cu, Se, Co, Ni and Cr [5].…”

Glycogen–gold nanohybrid escalates the potency of silymarin

“…[10][11][12][13] Annona reticulata (Annonaceae) is a small, deciduous tree commonly called Ramphal, Bullock's heart, or custard apple, which is native to India, West Indies, and tropical America, mainly cultivated for fruit production. 14,15 This plant is a good source of different phytochemicals like acetogenins, cycloreticulins A and B, cyclooctapeptides, cycloreticulin C, glabrin A, and cyclopeptides. [15][16][17] In this study, GNPs were synthesized using A. reticulata L. leaf aqueous extract as a reducing agent.…”