Assessment of humoral and cellular immunity after bivalent BNT162b2 vaccination and potential association with reactogenicity

Salvagno, Gian Luca; Pighi, Laura; Henry, Brandon Michael; Valentini, Myriam; Tonin, Beatrice; Bragantini, Damiano; Gianfilippi, Gianluca; Nitto, Simone De; Plebani, Mario; Lippi, Giuseppe

doi:10.21203/rs.3.rs-2477665/v1

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…We used log transformation of antibody titers for better normal distribution. Fisher's z-transformation was employed to mitigate the differences between the two mRNA vaccines (28,29). Linear fitting and correlation coefficient (R 2 ) calculation were conducted to examine the relationship between actigraphy measured objective sleep duration 3 days following booster vaccination and logtransformed antibody titers.…”

Section: Discussionmentioning

confidence: 99%

Association between sleep duration and antibody acquisition after mRNA vaccination against SARS-CoV-2

Izuhara,

Matsui,

Yoshiike

et al. 2023

Front. Immunol.

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IntroductionSleep enhances the antibody response to vaccination, but the relationship between sleep and mRNA vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is not fully understood.MethodsIn this prospective observational study, we investigated the influence of sleep habits on immune acquisition induced by mRNA vaccines against SARS-CoV-2 in 48 healthy adults (BNT-162b2, n=34; mRNA-1273, n=14; female, n=30, 62.5%; male, n=18, 37.5%; median age, 39.5 years; interquartile range, 33.0–44.0 years) from June 2021 to January 2022. The study measured sleep duration using actigraphy and sleep diaries, which covered the periods of the initial and booster vaccinations.ResultsMultivariable linear regression analysis showed that actigraphy-measured objective sleep duration 3 and 7 days after the booster vaccination was independently and significantly correlated with higher antibody titers (B=0.003; 95% confidence interval, 0.000–0.005; Beta=0.337; p=0.02), even after controlling for covariates, including age, sex, the type of vaccine, and reactogenicity to the vaccination. Associations between acquired antibody titer and average objective sleep duration before vaccination, and any period of subjective sleep duration measured by sleep diary were negligible.DiscussionLonger objective, but not subjective, sleep duration after booster vaccination enhances antibody response. Hence, encouraging citizens to sleep longer after mRNA vaccination, especially after a booster dose, may increase protection against SARS-CoV-2.Study registrationThis study is registered at the University Hospital Medical Information Network Center (UMIN: https://www.umin.ac.jp) on July 30, 2021, #UMIN000045009.

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Section: Discussionmentioning

confidence: 99%

Association between sleep duration and antibody acquisition after mRNA vaccination against SARS-CoV-2

Izuhara,

Matsui,

Yoshiike

et al. 2023

Front. Immunol.

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SARS-CoV-2 specific T-cell humoral response assessment after COVID-19 vaccination using a rapid direct real-time PCR amplification

Cosma

Galla

Padoan

et al. 2023

Clinical Chemistry and Laboratory Medicine (CCLM)

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Objectives The SARS-CoV-2 immune response is mediated by both humoral and cellular immunity. In this study, SARS-CoV-2 specific cellular immunity was tested by a novel direct real-time PCR (dRT-PCR) assay, targeting mRNA of CXCL10, and compared with respect to an ELISA measuring interferon gamma (IFN-γ) release. Methods Whole blood (Li–He) and serum samples were collected from 92 healthcare workers (HCW), with three doses of homologous (Pfizer/BioNTech, n=74) or heterologous (Pfizer/BioNTech and Vaxzevria or Moderna, n=18) vaccinations. Li–He samples were incubated with SCV2 PANEL-1-T-ACTIVATION (Hyris srl, Lodi, Italy), or CoV-2 IGRA TUBE ELISA (Euroimmune, Lubeck, Germany). CXCL10 mRNA expression was analyzed by bCube/bApp (Hyris), while IFN-γ was evaluated by quant-T-Cell SARS-CoV-2 ELISA (Euroimmune). Anti-SARS-CoV-2 S-RBD IgG levels were measured in sera using a CLIA assay (Snibe, Shenzen, China). Results Imprecision of dRT-PCR assay was found to be satisfactory, and the two methods for measuring T cell immunity to SARS-CoV-2 peptides agreed in 82/87 (94.2%) of results. At qualitative dRT-PCR analyses, 81 subjects (93.2%) resulted as reactive to SARS-CoV-2 peptides, 3 (3.4%) were borderline and 3 were negative (3.4%). At univariate and multivariate analyses of quantitative dRT-PCR mRNA of CXCL10 and IFN-γ release results showed no difference between HCW with previous infection, homologous/heterologous vaccination, or demographical features. Anti-SARS-CoV-2 S-RBD IgG was associated with the previous infection and the time between the last vaccination or positivity. Conclusions Direct RT-PCR appeared accurate for determining the presence or absence of immunoreactivity of SARS-CoV-2 specific T cells, especially when rapid analyses are required, such as for organ transplantation.

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Early kinetics of cellular immunity in recipients of bivalent BNT162b2 vaccine: a proof-of-concept study

Salvagno

Pighi

Henry

et al. 2023

Clinical Chemistry and Laboratory Medicine (CCLM)

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Assessment of humoral and cellular immunity after bivalent BNT162b2 vaccination and potential association with reactogenicity

Cited by 3 publications

References 19 publications

Association between sleep duration and antibody acquisition after mRNA vaccination against SARS-CoV-2

Association between sleep duration and antibody acquisition after mRNA vaccination against SARS-CoV-2

SARS-CoV-2 specific T-cell humoral response assessment after COVID-19 vaccination using a rapid direct real-time PCR amplification

Early kinetics of cellular immunity in recipients of bivalent BNT162b2 vaccine: a proof-of-concept study

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