Assessment of Human Skin Microcirculation and Its Endothelial Function Using Laser Doppler Flowmetry

Lenasi, Helena

doi:10.5772/27067

Cited by 23 publications

(50 citation statements)

References 89 publications

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“…There are no acceptable standard physiological tests available that would selectively increase cutaneous perfusion in the deeper plexus, without influencing superficial subpapillary microcirculation. 4 In the present study, the ability of the custom-designed iPPG system to determine perfusion in different cutaneous microcirculatory plexuses, superficial subpapillary and deep dermal, has been demonstrated by means of well-known physiological provocations: selectively increasing superficial perfusion (local heating and topical application of rubefacient) and simultaneously increasing perfusion in both superficial and deep microvasculature (arterial occlusion test).…”

Section: Limitations Of the Studymentioning

confidence: 85%

“…1 Assessment of microcirculation is of vital importance and provides essential information for the diagnosis of diseases because cutaneous the neuro-immunoendocrine system plays an important role in controlling physiological functions of the body, 2 serving as an early marker for chronic diseases, such as diabetes mellitus, Raynaud syndrome, metabolic syndrome, and even neuropathy with the potential application, ranging from routine testing of endothelial function to local anesthesia monitoring. 3,4 To reduce the influence of measuring procedures, a nonintrusive approach is essential. Over about the last four decades, a variety of contactless photonic methods have been developed and utilized for registering cutaneous circulation, such as laser Doppler flowmetry (LDF), 5 laser speckle contrast imaging (LSCI), 6,7 and tissue viability imaging (TiVi).…”

Section: Introductionmentioning

confidence: 99%

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Imaging photoplethysmography for clinical assessment of cutaneous microcirculation at two different depths

et al. 2016

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The feasibility of bispectral imaging photoplethysmography (iPPG) system for clinical assessment of cutaneous microcirculation at two different depths is proposed. The iPPG system has been developed and evaluated for in vivo conditions during various tests: (1) topical application of vasodilatory liniment on the skin, (2) skin local heating, (3) arterial occlusion, and (4) regional anesthesia. The device has been validated by the measurements of a laser Doppler imager (LDI) as a reference. The hardware comprises four bispectral light sources (530 and 810 nm) for uniform illumination of skin, video camera, and the control unit for triggering of the system. The PPG signals were calculated and the changes of perfusion index (PI) were obtained during the tests. The results showed convincing correlations for PI obtained by iPPG530 nm and LDI at (1) topical liniment (r = 0.98) and (2) heating (r = 0.98) tests. The topical liniment and local heating tests revealed good selectivity of the system for superficial microcirculation monitoring. It is confirmed that the iPPG system could be used for assessment of cutaneous perfusion at two different depths, morphologically and functionally different vascular networks, and thus utilized in clinics as a cost-effective alternative to the LDI.

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Section: Limitations Of the Studymentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Imaging photoplethysmography for clinical assessment of cutaneous microcirculation at two different depths

et al. 2016

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“…Hyperspectral imaging is among them and has already proven its clinical potential in detection of skin malformations 3 , however its use for skin blood oxygen saturation detection is still challenging because of structural non-uniformity of skin and complex cutaneous blood circulation 4 . Traditionally, development and evaluation of such methods require tissue phantom measurements; however even advanced phantoms still barely mimic the real skin with its complex microvasculature.…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral evaluation of skin blood oxygen saturation at baseline and during arterial occlusion

Marcinkevičs

Rubīns

Grabovskis

et al. 2018

Biophotonics: Photonic Solutions for Better Health Care VI

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Skin capillary blood oxygen saturation is a clinically important diagnostic parameter, which provides valuable information for timely treatment of pathological conditions e.g. sepsis, hypoxemia or decompression illness. Hyperspectral imaging is non-invasive optical techniques with high clinical potential, however its use for skin blood oxygen saturation detection is still challenging, therefore in the present study, a method for in-vivo manipulation of skin oxygen saturation was developed, and reliability of the method evaluated by means of hyperspectral imaging in detection of oxygen saturation. In order to produce alterations of skin capillary blood parameters and oxygen saturation, the proximal phalanx of the right middle finger was occluded with a pneumatic cuff for 25 minutes. During the last minute of occlusion, the hyperspectral cubes (HIS) of both occluded and intact finger were captured, and capillary blood sample was collected for analysis with portable whole blood analyzer (REF). The group mean values for SaO 2 in intact finger skin was HIS: 89.46%±8.79% versus REF: 95.13±1.46 % and in occluded finger HSI: 25.85% ±14.00%, versus REF: 22.73±9.09 % displaying a small difference between two independent techniques, which indicate the reliability of finger occlusion model.

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“…A pele é facilmente acessível [1] e potencialmente representativa [2] para a avaliação dos mecanismos de função e disfunção da microcirculação periférica [3][4][5] em particular quando são usadas tecnologias não invasivas, como a Fluxometria por Laser Doppler (FLD). A FLD está entre as tecnologias mais amplamente utilizadas para este propósito [6] , fornecendo um sinal complexo que pode ser decomposto em componentes com intervalos de frequência característicos, por meio de ferramentas de análise espectrais, tais como a transformada rápida de Fourier, a modelação autorregressiva e a análise de ôndula [7] .…”

Section: Introductionunclassified

“…The skin is easily accessible [1] and potentially representative [2] for the evaluation of peripheral microcirculation function and dysfunction mechanisms [3][4][5] , in particular when noninvasive technologies such as Laser Doppler Flowmetry (LDF) are used. LDF is among the most widely employed technologies for this purpose [6] , providing a complex signal which can be decomposed into components with characteristic frequency ranges by means of spectral analysis tools, such as the fast Fourier transform, autoregressive modelling and wavelet analysis [7] .…”

Section: Introductionmentioning

confidence: 99%

The wavelet transform as a tool for the characterization of the vascular response in the human lower limb

Silva¹,

Ferreira²,

Buján³

et al. 2014

BBR

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75 Biomedical and Biopharmaceutical Research J o r n a l d e I n v e s t i g a ç ã o B i o m é d i c a e B i o f a r m a c ê u t i c aThe wavelet transform as a tool for the characterization of the vascular response in the human lower limb AbstractIn recent years, cutaneous microcirculation gained considerable interest for the assessment of microcirculation function and dysfunction mechanisms. Laser Doppler Flowmetry (LDF) is a noninvasive and widely employed technology to study microcirculation. It provides a complex signal that may be decomposed by wavelet analysis into several components at characteristic frequency ranges. These are related to the heart (0.6 Hz to 2 Hz), respiration (0.15 Hz to 0.6 Hz), myogenic activity in the vessel wall (0.052 Hz to 0.15 Hz), sympathetic activity (0.021 Hz to 0.052 Hz) and endothelial (nitric oxide-mediated) activity (0.0095 Hz to 0.021 Hz). A group of 30 healthy (22.3 ± 3.1) years old subjects , giving previously informed consent, tested two perfusion-restricting protocols which allowed us to study the circulatory response in the lower limb: (1) passive leg elevation from the supine position for 10 minutes, (2) inflated cuff occlusion at the ankle level for 3 minutes from the sitting position. The LDF signal was partitioned using a Morlet wavelet model. Data analysis revealed significantly lower components' amplitudes for both protocols during provocation, with consistently lower values for protocol 1 in relation to protocol 2. Significant gender differences were found for protocol 1 but not for protocol 2. These results demonstrate the usefulness of the wavelet analysis to assess each LDF signal component as part of the physiological response to the lower limb microcirculation regulation.

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Assessment of Human Skin Microcirculation and Its Endothelial Function Using Laser Doppler Flowmetry

Cited by 23 publications

References 89 publications

Imaging photoplethysmography for clinical assessment of cutaneous microcirculation at two different depths

Imaging photoplethysmography for clinical assessment of cutaneous microcirculation at two different depths

Hyperspectral evaluation of skin blood oxygen saturation at baseline and during arterial occlusion

The wavelet transform as a tool for the characterization of the vascular response in the human lower limb

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