Assessment of human ovarian follicular fluid derived mesenchymal stem cells in chitosan/PCL/Zn scaffold for bone tissue regeneration

Chandramohan, Yamini; Jeganathan, Kavya; Sivanesan, Sanjana; Koka, Pavani; Amritha, Trikkur Madom Seetaraman; Vimalraj, Selvaraj; Dhanasekaran, Anuradha

doi:10.1016/j.lfs.2020.118502

Cited by 22 publications

(9 citation statements)

References 47 publications

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“…53 PCL scaffolds used either singly or in combination with other polymers have been investigated in the differentiation of adult stem cells from various sources to different lineages. [54][55][56] Similar to the present study, Tian et al 57 investigated cardiomyocyte differentiation of MSCs on scaffolds using cardiomyocyte differentiation agents. These authors cul- with TGF-β even in the absence of nanofibrous scaffolds.…”

Section: Discussionsupporting

confidence: 59%

“…The authors found out that Wnt/β‐catenin signaling was upregulated during the cardiomyocyte differentiation of miPSCs 53 . PCL scaffolds used either singly or in combination with other polymers have been investigated in the differentiation of adult stem cells from various sources to different lineages 54–56 . Similar to the present study, Tian et al 57 investigated cardiomyocyte differentiation of MSCs on scaffolds using cardiomyocyte differentiation agents.…”

Section: Discussionmentioning

confidence: 52%

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Polycaprolactone‐co‐polylactic acid nanofiber scaffold in combination with 5‐azacytidine and transforming growth factor‐β to induce cardiomyocyte differentiation of adipose‐derived mesenchymal stem cells

Tambrchi

Mahdavi

Daliri‐Joupari

et al. 2022

Cell Biochemistry & Function

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Adipose‐derived mesenchymal stem cells (Ad‐MSCs) are promising candidates for cardiac repair/regeneration. The application of copolymer nanoscaffolds has received great attention in tissue engineering to support differentiation and functional tissue organization toward effective tissue regeneration. The objective of the current study was to develop functional and bioactive scaffolds by combining polycaprolactone (PCL) and polylactic acid (PLA) for cardiomyocyte differentiation of human Ad‐MSC (hAd‐MSCs) in the absence or presence of 5‐azacytidine and transforming growth factor‐β (TGF‐β). To that end, the human MSCs were extracted from human adipose tissue (AD). The cardiomyocyte differentiation potency of hAd‐MSCs was evaluated on the novel synthetic PCL/PLA nanofiber scaffolds prepared in the absence and presence of 5‐azacytidine and TGF‐β supplements. A PCL/PLA nanofibrous scaffold was fabricated using the electrospinning method and its nanotopography and porous structure were characterized using scanning electron microscopy. In addition, the attachment of hAd‐MSCs on the PCL/PLA scaffolds was semiquantitatively investigated. Compared with other treatments, the PCL/PLA nanofibrous scaffold supplemented with both 5‐azacytidine and TGF‐β was observed to differentiate hAd‐MSCs into cardiomyocytes at Day 21 as evidenced by real‐time PCR for cardiac‐specific genes including cardiac troponin I (cTnI), GATA4, MYH7, and NKX2.5. In addition, flow cytometric analysis of cTnI‐positive cells demonstrated that the cardiomyocyte differentiation of hAd‐MSCs was more efficient on the PCL/PLA nanofibrous scaffold supplemented with both 5‐azacytidine and TGF‐β than it was in the other treatment groups. Generally speaking, the results show that PCL/PLA nanofibrous scaffolds may be applied as a platform for efficient differentiation of hAd‐MSCs into functional cardiomyocytes.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 52%

Polycaprolactone‐co‐polylactic acid nanofiber scaffold in combination with 5‐azacytidine and transforming growth factor‐β to induce cardiomyocyte differentiation of adipose‐derived mesenchymal stem cells

Tambrchi

Mahdavi

Daliri‐Joupari

et al. 2022

Cell Biochemistry & Function

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“…The above results indicated that GCs possessed osteogenic potential both in vitro and in vivo and therefore could be used for bone regeneration [143]. Chandramohan et al [144] proposed a scaffold based on chitosan and polycaprolactone (PCL) for ovarian stem cell engineering. The cells were collected from human follicular fluid and exhibited properties of MSCs while seeded on the chitosan/PCL scaffold, coated with zinc divalent ions to impart osteogenic properties.…”

Section: Application Of Ovarian Follicular Stem Cells In Translational Medicinementioning

confidence: 97%

“…The cells were collected from human follicular fluid and exhibited properties of MSCs while seeded on the chitosan/PCL scaffold, coated with zinc divalent ions to impart osteogenic properties. As a result, calcium deposition and alkaline phosphatase activity were increased, together with the expression of Runx2, osteonectin or osteocalcin, indicating that such a scaffold was compatible with MSCs and could be used in bone tissue engineering [144]. GCs were also demonstrated as a useful tool for obtaining functional oocytes.…”

Section: Application Of Ovarian Follicular Stem Cells In Translational Medicinementioning

confidence: 99%

Human Granulosa Cells—Stemness Properties, Molecular Cross-Talk and Follicular Angiogenesis

Dompe

Kulus

Stefańska

et al. 2021

Cells

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The ovarian follicle is the basic functional unit of the ovary, comprising theca cells and granulosa cells (GCs). Two different types of GCs, mural GCs and cumulus cells (CCs), serve different functions during folliculogenesis. Mural GCs produce oestrogen during the follicular phase and progesterone after ovulation, while CCs surround the oocyte tightly and form the cumulus oophurus and corona radiata inner cell layer. CCs are also engaged in bi-directional metabolite exchange with the oocyte, as they form gap-junctions, which are crucial for both the oocyte’s proper maturation and GC proliferation. However, the function of both GCs and CCs is dependent on proper follicular angiogenesis. Aside from participating in complex molecular interplay with the oocyte, the ovarian follicular cells exhibit stem-like properties, characteristic of mesenchymal stem cells (MSCs). Both GCs and CCs remain under the influence of various miRNAs, and some of them may contribute to polycystic ovary syndrome (PCOS) or premature ovarian insufficiency (POI) occurrence. Considering increasing female fertility problems worldwide, it is of interest to develop new strategies enhancing assisted reproductive techniques. Therefore, it is important to carefully consider GCs as ovarian stem cells in terms of the cellular features and molecular pathways involved in their development and interactions as well as outline their possible application in translational medicine.

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“…The coordination of empty orbitals of Zn 2+ with lone pair of electrons of NH 2 , OH and COO − groups of CMCh leads to the rapid formation of CMCh-Zn complex after simply mixing a solution of modified chitosan and Zn(NO 3 ) 2 •6H 2 O salt together within the pH range of 5.3-7.0. These CMCh-Zn hydrogels could be used for bone TE applications as Zn is an essential element in bone homeostasis and has been used as a therapeutic agent in bone regeneration [14,15]. However, the use of metal ions should be carefully considered as they can be toxic if exceeding tolerable concentrations.…”

Section: Classification Of Supramolecular Hydrogels Based On Their Comentioning

confidence: 99%

Supramolecular Hydrogels for Protein Delivery in Tissue Engineering

Lyu

Azevedo

2021

Molecules

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Therapeutic proteins, such as growth factors (GFs), have been used in tissue engineering (TE) approaches for their ability to provide signals to cells and orchestrate the formation of functional tissue. However, to be effective and minimize off-target effects, GFs should be delivered at the target site with temporal control. In addition, protein drugs are typically sensitive water soluble macromolecules with delicate structure. As such, hydrogels, containing large amounts of water, provide a compatible environment for the direct incorporation of proteins within the hydrogel network, while their release rate can be tuned by engineering the network chemistry and density. Being formed by transient crosslinks, afforded by non-covalent interactions, supramolecular hydrogels offer important advantages for protein delivery applications. This review describes various types of supramolecular hydrogels using a repertoire of diverse building blocks, their use for protein delivery and their further application in TE contexts. By reviewing the recent literature on this topic, the merits of supramolecular hydrogels are highlighted as well as their limitations, with high expectations for new advances they will provide for TE in the near future.

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Assessment of human ovarian follicular fluid derived mesenchymal stem cells in chitosan/PCL/Zn scaffold for bone tissue regeneration

Cited by 22 publications

References 47 publications

Polycaprolactone‐co‐polylactic acid nanofiber scaffold in combination with 5‐azacytidine and transforming growth factor‐β to induce cardiomyocyte differentiation of adipose‐derived mesenchymal stem cells

Polycaprolactone‐co‐polylactic acid nanofiber scaffold in combination with 5‐azacytidine and transforming growth factor‐β to induce cardiomyocyte differentiation of adipose‐derived mesenchymal stem cells

Human Granulosa Cells—Stemness Properties, Molecular Cross-Talk and Follicular Angiogenesis

Supramolecular Hydrogels for Protein Delivery in Tissue Engineering

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