Assessment of human muscle blood perfusion with single-fiber laser Doppler flowmetry

Kvernebo, Knut; Staxrud, L.E.; Salerud, E. Göran

doi:10.1016/0026-2862(90)90050-2

Cited by 27 publications

(26 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Intramuscular reactive hyperaemia measurements with the laser Doppler have been used and validated in previous studies [11,12,28]. Calculation of the relative increase in intramuscular perfusion allows correction for differences in baseline intramuscular perfusion.…”

Section: Discussionmentioning

confidence: 99%

“…Calculation of the relative increase in intramuscular perfusion allows correction for differences in baseline intramuscular perfusion. This is important, because previous studies have shown that intramuscular laser Doppler perfusion at rest shows considerable spatial variation at different measurement sites [11,12]. Our experiments were performed in conscious, healthy subjects and so slight changes in the probe measurement angle and depth could not be avoided.…”

Section: Discussionmentioning

confidence: 99%

“…After 5 minutes of resting laser Doppler recording, the cuff was rapidly inflated to 200 mm Hg. This pressure was maintained for 3 minutes, which was shown by others [11] to give a maximal hyperaemic response. After release of the cuff, intramuscular reactive hyperaemia was recorded.…”

Section: Methodsmentioning

confidence: 99%

“…The same technique can also be applied to the vasculature of human skeletal muscle. If the probe position remains unchanged, the reproducibility of intramuscular reactive hyperaemia measurements after arterial occlusion has been shown to be excellent [11,12].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Physiological hyperinsulinaemia increases intramuscular microvascular reactive hyperaemia and vasomotion in healthy volunteers

et al. 2004

View full text Add to dashboard Cite

Aims/hypothesis. Insulin possesses vasodilatory actions that may be important in regulating its access to insulin-sensitive tissues. Our study aims to directly measure changes in response to insulin in the human skeletal muscle microcirculation. Measurement was by an implanted laser Doppler probe. Methods. We investigated changes in intramuscular and skin microvascular perfusion in 12 healthy individuals during a hyperinsulinaemic and a control clamp. We determined leg blood flow with plethysmography, finger skin functional capillary recruitment with capillaroscopy, endothelium-(in)dependent vasodilation by iontophoresis of acetylcholine and sodium nitroprusside, and leg intramuscular reactive hyperaemia and vasomotion with laser Doppler measurements. Results. Compared to the control study, hyperinsulinaemia (416±82 pmol/l) caused: (i) an increase in leg blood flow (1.0±1.0 vs 0.1±0.6 ml·min −1 ·100 ml, p<0.05); (ii) an increase in finger skin capillary recruitment (14.9±10.1 vs -5.6±11.0%, p<0.01); (iii) no change in baseline laser Doppler perfusion either in finger skin or leg muscle; (iv) a tendency to increase acetylcholine-mediated vasodilation (475±534 vs 114±337%, p=0.07) with no change in sodiumnitroprusside-mediated vasodilation (p=0.2) in finger skin; (v) an increase in intramuscular reactive hyperaemia (423±507 vs 0±220%, p<0.01); and (vi) a decrease in time needed to reach peak intramuscular perfusion (−3.6±3.0 vs 1.1±3.1 s, p<0.01). In addition, hyperinsulinaemia induced an increase in intramuscular vasomotion by increasing the contribution of frequencies between 0.01 and 0.04 Hz (p<0.05 for all), which probably represents increased endothelial and neurogenic activity. Conclusions/interpretation. Physiological hyperinsulinaemia not only stimulates total blood flow and skin microvascular perfusion, but also augments human skeletal muscle microvascular recruitment and vasomotion as detected directly by laser Doppler measurements.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Physiological hyperinsulinaemia increases intramuscular microvascular reactive hyperaemia and vasomotion in healthy volunteers

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Given that, a representative estimation of the flow in the tissue could not be obtained by measuring the cell flux in such a small volume because of the probe used. Once a stable signal had been reached, the mean of five measurements (performed at five different sites that were near each other) was used in order to reduce any measuring error [6,22].…”

Section: Discussionmentioning

confidence: 99%

Laser Doppler Evaluation of Microcirculation Behaviour during an Ischaemia-Reperfusion Injury

Rocca¹,

Giavaresi²,

Fini³

et al. 1998

Eur Surg Res

View full text Add to dashboard Cite

Laser Doppler flowmetry (LDF) was tested in an experimental ischaemic model on rat limbs to evaluate the degree of ischaemia and to find a possible correlation with values obtained with this device and prognosis. Under general anaesthesia, 40 Wistar rats were submitted to 4 h and 30 min of ischaemia of the left hind limb. Ten rats formed the control group (group 1). Two enzymes, native superoxide dismutase (SOD) and SOD modified with polyethylene glycol, were employed in 15 rats each (groups 2 and 3). Data were collected by means of LDF both in the sole and muscles before ischaemia (steady state), during ischaemia and at the beginning of reperfusion, and only in the sole after 1 h of reperfusion. A range of predictive (95%) perfusion values (PU) for limb healing or necrosis was identified at the beginning of reperfusion. During ischaemia, PU changed from 0 to 10, both in the sole and in the muscle. A three-factor ANOVA (site, group, time) did not show interaction of these factors with PU (F = 1.655; p = 0.195), even if every single effect was significant (p < 0.0005). A two-factor ANOVA (group, time) showed a significant interaction of these factors with PU (F = 4.079; p = 0.019). The logistic regression between the reperfusion PU of each site and the survival of the limb was observed at the beginning and after 1 h of reperfusion in the sole only. Furthermore, a correlation between sole and muscle PU at the steady state and at the beginning of the reperfusion period was observed. The results showed the effectiveness of LDF, which can be considered a quite reliable tool to evaluate the degree of ischaemia and to have a good correlation with prognosis in this kind of experiments.

show abstract

Carvedilol reduces ischaemic skeletal muscle necrosis

Hvaal

Mathisen

Svindland

et al. 1999

Journal Orthopaedic Research

View full text Add to dashboard Cite

Carvedilol is an alpha1 and nonselective beta-adrenergic receptor antagonist with antioxidative properties known to protect against reperfusion injury in the heart, brain, and kidneys. The aim of this study was to test the hypothesis that carvedilol improves postischaemic reperfusion and tissue survival in skeletal muscle. Sixteen Wistar rats underwent tourniquet ischaemia of the left hindlimb for 3 hours and 15 minutes at 27 degrees C. Single-fiber laser Doppler probes were inserted in the left and right anterior tibial muscles, and microvascular perfusion was measured until 2 hours after removal of the tourniquet. Perfusion indices for each 15-minute interval were calculated for the left hindlimb (tourniquet ischaemia) by dividing the postischaemic by the pre-ischaemic laser Doppler flowmetry values, and the geometrical areas under the curves representing a plot of perfusion index relative to time, measured in arbitrary units, were compared. Laser Doppler flowmetry values for the right anterior tibial muscle were compared. Tissue damage was measured by histomorphometry of necrotic areas and no-reflow zones in cross sections from the anterior tibial muscle 72 hours after ischaemia. Neutrophils were counted in the same sections. The treatment group received 1 mg carvedilol/kg body weight before ischaemia and 1 mg/kg immediately before removal of the tourniquets. The areas under the curves representing the plot of perfusion index relative to time were larger for the rats treated with carvedilol: 9.5 compared with 3.0 arbitrary units (p = 0.0003). Treatment did not change the laser Doppler flowmetry values for the right hindlimbs. The histomorphometric areas of necrosis in cross sections from the muscles were reduced from 88% (38-96%) in the control animals to 41% (7-85%) in those treated with carvedilol (p = 0.01), and the area of no-reflow was reduced from 20% (2-52%) to 0% (0-7%) (p = 0.006). The number of neutrophils did not differ between groups. The study supports the hypothesis that carvedilol improves early reperfusion and protects skeletal muscle subjected to 3 hours and 15 minutes of ischaemia.

show abstract

Assessment of human muscle blood perfusion with single-fiber laser Doppler flowmetry

Cited by 27 publications

References 15 publications

Physiological hyperinsulinaemia increases intramuscular microvascular reactive hyperaemia and vasomotion in healthy volunteers

Physiological hyperinsulinaemia increases intramuscular microvascular reactive hyperaemia and vasomotion in healthy volunteers

Laser Doppler Evaluation of Microcirculation Behaviour during an Ischaemia-Reperfusion Injury

Carvedilol reduces ischaemic skeletal muscle necrosis

Contact Info

Product

Resources

About