Assessment of human gingival fibroblast interaction with dental implant abutment materials

Rutkūnas, Vygandas; Bukelskienė, Virginija; Sabaliauskas, Vaidotas; Balčiūnas, Evaldas; Malinauskas, Mangirdas; Baltriukienė, Daiva

doi:10.1007/s10856-015-5481-8

Cited by 43 publications

(42 citation statements)

References 44 publications

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“…Vinculin is a cytoskeletal protein involved in formation of focal adhesion [35], and for this reason we aimed to quantitatively and qualitatively analyse it. Previous studies have indicated conflicting results with respect to HGFs' expression of vinculin [20,36,37].…”

Section: Discussionmentioning

confidence: 98%

Experimental implantoplasty outcomes correlate with fibroblast growth in vitro

et al. 2020

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Background: Implantoplasty is an option in peri-implantitis treatment, but little is known about the effect on the soft tissue. The aim of the study was to characterize surface roughness following experimental implantoplasty and to examine its effect on human fibroblast growth and secretion of selected proteins.Methods: Titanium grade IV coins were mechanically treated with six different rotating bur sequences; diamond burs or carbide burs alone, or followed by either Arkansas stone bur or silicone burs. Machined and rough-surface sandblasted, acid-etched (SLA) coins were used as control. The surface topography was characterized by scanning electron microscope and profilometer. Human gingival fibroblasts from two donors were cultured on the coins to quantify the effect on cell morphology, growth, and protein secretion by confocal microscopy and multiplex immunoassay. Results: All surface roughness parameters were lower for the surfaces treated with experimental implantoplasty than for the SLA surface, and the sequence of carbide burs followed by silicone burs rendered the least rough surface of the test groups. The implantoplasty procedures changed the elemental composition of the titanium surface. High surface roughness showed a weak to moderate negative correlation to fibroblast growth, but induced a higher secretion of VEGF, IL-6 and MCP-3 to the cell medium compared to the least rough surfaces of the test groups. At day 30 fibronectin levels were higher in the SLA group. Conclusions: The surface roughness following implantoplasty demonstrated a weak to moderate negative correlation with the growth of fibroblasts. The addition of Arkansas stone and silicon burs to the experimental implantoplasty bur protocol rendered an initial increase in fibroblast growth. Implantoplasty altered the elemental composition of the titanium surface, and had an effect on the fibroblast cytokine secretion and fibronectin levels.

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Section: Discussionmentioning

confidence: 98%

Experimental implantoplasty outcomes correlate with fibroblast growth in vitro

et al. 2020

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“…Previous studies have indicated conflicting results with respect to HGFs' expression of vinculin. 20,36,37 Since the effect of the experimental implantoplasty treatment on the fibroblast growth was limited beyond day 6 in the present study, the analyses of cytokine secretion to the cell medium was performed at the two earliest time points only. Also the DB and CB sequences were left out of the Luminex analysis since these rendered the roughest surfaces of the experimental implantoplasty treatments, and would therefore not be considered in a clinical setting.…”

Section: Discussionmentioning

confidence: 99%

Experimental implantoplasty outcomes correlate with fibroblast growth in vitro

Maal

Ellingsen

Reseland

et al. 2019

Preprint

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Background Implantoplasty is an option in peri-implantitis treatment, but little is known about the effect on the soft tissue. The aim of the study was to characterize surface roughness following experimental implantoplasty and to examine its effect on human fibroblast growth and the secretion of selected proteins.Methods Titanium grade IV coins were mechanically treated with six different rotating bur sequences; diamond burs or carbide burs alone, or followed by either Arkansas stone bur or silicone burs. Machined and rough-surface sandblasted, acid-etched (SLA) coins were used as control. The surface topography was characterized by scanning electron microscope and profilometer. Human gingival fibroblasts from two donors were cultured on the coins to quantify the effect on cell morphology, growth and protein secretion by confocal microscopy and multiplex immunoassay.Results All surface roughness parameters were lower for the surfaces treated with experimental implantoplasty than for the SLA surface, and the sequence of carbide burs followed by silicone burs rendered the least rough surface of the test groups. The implantoplasty procedures changed the elemental composition of the titanium surface. High surface roughness showed a weak to moderate negative correlation to fibroblast growth, but induced a higher secretion of VEGF, IL-6 and MCP-3 to the cell medium compared to the least rough surfaces of the test groups. At day 30 fibronectin levels were higher in the SLA group.Conclusions The surface roughness following implantoplasty demonstrated a weak to moderate negative correlation with the growth of fibroblasts. The addition of Arkansas stone and silicon burs to the experimental implantoplasty bur protocol rendered an initial increase in fibroblast growth. Implantoplasty altered the elemental composition of the titanium surface, and had an effect on the fibroblast cytokine secretion and fibronectin levels.

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“…FAK is a vital non-receptor tyrosine kinase that regulates cell adhesion signaling and mechanosensing. 53 Vinculin is a key regulator of adhesion strength and cell migration. 54 FAK gene expression in HGFs was increased in all three experimental groups when compared with the control group, particularly on ZrO 2 -P/C (p < 0.01) and ZrO 2 -P (p < 0.05).…”

Section: Gene Expression and Protein Synthesis In Hgfsmentioning

confidence: 99%

Biofunctionalization of zirconia with cell-adhesion peptides via polydopamine crosslinking for soft tissue engineering: effects on the biological behaviors of human gingival fibroblasts and oral bacteria

et al. 2020

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Rapid soft tissue integration is essential for long-term dental implant success. Zirconia is increasingly used as an abutment material owing to its excellent aesthetic properties and biocompatibility; however, it is bioinert, and tissue integration is poor. We developed a feasible surface modification method, exploiting the reactivity of polydopamine (PDA) films to immobilize cell-adhesion peptides (Arg-Gly-Asp, RGD) onto zirconia abutment surfaces. Further, we evaluated the effect thereof on human gingival fibroblast (HGF) behavior and oral bacterial adhesion, which influence the peri-implant soft tissue seal. HGF responses to linear KGGRGDSP and cyclic RGDfK sequences were compared. PDA deposition and covalent coupling of RGD were verified by X-ray photoelectron spectroscopy and fluorescence microscopy. The biological behaviors of HGFs on the modified zirconia; i.e., adhesion, spreading, proliferation, gene and protein expression, were elucidated. Biofunctionalization of zirconia with the adhesion peptides significantly enhanced the biological activities of HGFs. Cyclic RGD induced slightly improved cell attachment, spreading, and proliferation, but similar cell differentiation when compared to linear RGD peptides. To assess their antimicrobial properties, the different substrates were exposed to cultures of the early colonizer Streptococcus mutans or the periodontal pathogen Porphyromonas gingivalis, and bacterial adhesion was evaluated by scanning electron microscopy and live/dead staining.PDA and PDA-RGD coatings decreased zirconia surface colonization by both bacterial species to similar extents. Thus, PDA-RGD-functionalized zirconia modulates specific HGF responses, while maintaining the antimicrobial activity of the PDA coating. The selective bio-interaction pattern of this surface modification holds great promise for improving soft-tissue integration around zirconia abutments in clinical applications.

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Assessment of human gingival fibroblast interaction with dental implant abutment materials

Cited by 43 publications

References 44 publications

Experimental implantoplasty outcomes correlate with fibroblast growth in vitro

Experimental implantoplasty outcomes correlate with fibroblast growth in vitro

Experimental implantoplasty outcomes correlate with fibroblast growth in vitro

Biofunctionalization of zirconia with cell-adhesion peptides via polydopamine crosslinking for soft tissue engineering: effects on the biological behaviors of human gingival fibroblasts and oral bacteria

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