Assessment of Histopathological Changes in the Liver and Spleen of Mice Infected with Salmonella typhi Following Treatment with Ciprofloxacin

Abstract

This study evaluated the toxicity of ciprofloxacin to spleen and liver when used for the treatment of mice infected with S. typhi for seven days. The dose concentration used in these experiments was 100mg/kg. Mice were divided into two groups . The first group (negative control) was not given ciprofloxacin, but rather a sterile phosphate buffer solution (PBS) as an alternative. Ciprofloxacin was administered to the second group. After seven days , the animals were sacrificed and organs (liver and spleen) were collected . The histopathological examination showed normal hepatocytes in the liver and normal structure of spleen cells in animals of control group . However, the treated group showed dilated and congested blood vessels with perivascular inflammatory cell cuffing and acute cell swelling in the liver, as well as white pulp activation with an increased number of megakaryocyte cells in the spleen. Therefore, the current study suggests that the concentration of 100 mg/kg of ciprofloxacin is considered to be toxic to hepatocytes and splenocytes of mice during the treatment period.