Assessment of haemic neoplasia in different soft shell clam Mya arenaria populations from eastern Canada by flow cytometry

Delaporte, Maryse; Synard, Stéphanie; Pariseau, Julie; McKenna, Patricia; Tremblay, Réjean; Davidson, Jeff; Berthe, Franck

doi:10.1016/j.jip.2007.12.005

Cited by 47 publications

(62 citation statements)

References 16 publications

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“…The FCM analysis of the clam population studied showed that 21% of tested clams were affected, which confirms previous reports by Delaporte et al (2008) and Siah et al (2008) suggesting that HN is widely spread among North River clams.…”

Section: Discussionsupporting

confidence: 88%

“…Samples: After individual assessment of all clams by FCM, clams were assigned to 1 of 7 groups based on the percentages of tetraploid cells in the haemolymph, for TM-PERT analysis (Table 1). Clams with < 5% tetraploid cells were considered as HN-negative controls based on Delaporte et al (2008). Three clams were selected for inclusion in each of the 6 subsequent groups, except the ~70% group, which contained only 2 clams.…”

Section: Methodsmentioning

confidence: 99%

“…The detailed composition of the experimental groups and the percentages of tetraploid cells in clams are listed in Table 1. Clams containing between > 5% and < 20% tetraploid cells were presumed doubtful, and those with > 20% tetraploid cells were presumed positive based on Delaporte et al (2008).…”

Section: Methodsmentioning

confidence: 99%

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Reverse transcriptase activity associated with haemic neoplasia in the soft-shell clam Mya arenaria

AboElkhair

Siah²,

Clark³

et al. 2009

Dis. Aquat. Org.

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Reverse transcriptase (RT) activity has been reported in bivalves affected by haemic neoplasia (HN). Since all retroviruses have RT, detection of RT activity was regarded as evidence for the retroviral etiology of HN. This study investigates the relationship between RT levels and the progress of HN as indicated by percentages of tetraploid cells in soft-shell clams Mya arenaria. The percentages of tetraploid cells were estimated by flow cytometry, and the RT levels were quantified using TaqMan product-enhanced RT (TM-PERT) assay. Results demonstrated that the amount of RT was positively correlated with the percentage of tetraploid cells circulating in clam haemolymph (R 2 = 0.974, p < 0.001). Compared to HN-negative clams (< 5% tetraploid cells), 2 stages with significantly elevated levels of RT activity were observed: the first stage at ~10 to ~20% tetraploid cells, and the second at ~30 to ~80% tetraploid cells (p < 0.01). These data support the well established fact from mammalian models that transformed cells express high levels of non-telomeric RT. The observed increase in RT levels at ~30% tetraploidy coincides with previously reported p53 gene expression. Taken together, this could indicate that using RT levels as an indicator of HN, ≥30% tetraploidy is the stage at which the disease process undergoes a change, and perhaps becomes irreversible.

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Section: Discussionsupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Reverse transcriptase activity associated with haemic neoplasia in the soft-shell clam Mya arenaria

AboElkhair

Siah²,

Clark³

et al. 2009

Dis. Aquat. Org.

View full text Add to dashboard Cite

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“…The frequency of binucleated haemocytes was higher in neoplastic cockles than in non-neoplastic cockles, as described before by Barber (2004). Previous studies in molluscs have shown an increase in the amount of DNA in neoplastic cells (da Silva et al, 2005;Delaporte et al, 2008;Le Grand et al, 2010;Moore et al, 1991). Exposure to genotoxic pollutants can cause DNA damage giving rise to abnormal cells and possible neoplastic cells (Dopp et al, 1996).…”

Section: Medulissupporting

confidence: 62%

Biomarkers and transcription levels of cancer-related genes in cockles Cerastoderma edule from Galicia (NW Spain) with disseminated neoplasia

et al. 2013

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“…Based on flow cytometry examination, the abnormal circulating haemocytes in neoplastic clams are tetraploid, displaying atypical acrocentric and telocentric chromosomes, which are not present in normal healthy cells (Reno et al 1994). DN in the soft-shell clam is usually accepted as a tetraploid disorder (Reno et al 1994;Delaporte et al 2008). However, our previous study showed no correlation between xenobiotic exposure and tetraploidy status in haemocytes of soft-shell clams (Pariseau et al 2009).…”

Section: Introductionmentioning

confidence: 87%

Effects of pesticide compounds (chlorothalonil and mancozeb) and benzo[a]pyrene mixture on aryl hydrocarbon receptor, p53 and ubiquitin gene expression levels in haemocytes of soft-shell clams (Mya arenaria)

et al. 2011

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The aim of this study is to investigate the effects of the pesticides/polycyclic aromatic hydrocarbon mixture on aryl hydrocarbon receptor (AhR), p53 and ubiquitin mRNA level in haemocytes of Mya arenaria exposed to a mixture of chlorothalonil, mancozeb and benzo[a]pyrene (BaP) for 48 and 72 h. AhR, p53 and ubiquitin gene expression levels were quantified using quantitative Real-time PCR. For robust and accurate quantification of transcripts, suitable housekeeping genes were selected from four sets of ribosomal and elongation factors transcripts previously sequenced from Mya arenaria using geNorm open source software. Quantitative Real-time PCR data exhibited a significantly high expression of AhR after 72 h of exposure (P ≤ 0.05). p53 gene expression seems to be up-regulated by the mixture after 48 h, however not significantly; but the level of p53 mRNA is down-regulated by the xenobiotics between 48 and 72 h after exposure. This study postulates that AhR mRNA levels could be used as an indicator of the exposure of clams' haemocytes to a mixture of xenobiotics such as chlorothalonil, mancozeb and BaP. However, further studies have to be pursued in order to unravel the molecular mechanisms involved in the p53 signaling pathway.

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Assessment of haemic neoplasia in different soft shell clam Mya arenaria populations from eastern Canada by flow cytometry

Cited by 47 publications

References 16 publications

Reverse transcriptase activity associated with haemic neoplasia in the soft-shell clam Mya arenaria

Reverse transcriptase activity associated with haemic neoplasia in the soft-shell clam Mya arenaria

Biomarkers and transcription levels of cancer-related genes in cockles Cerastoderma edule from Galicia (NW Spain) with disseminated neoplasia

Effects of pesticide compounds (chlorothalonil and mancozeb) and benzo[a]pyrene mixture on aryl hydrocarbon receptor, p53 and ubiquitin gene expression levels in haemocytes of soft-shell clams (Mya arenaria)

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