Assessment of grating-based X-ray phase-contrast CT for differentiation of invasive ductal carcinoma and ductal carcinoma in situ in an experimental ex vivo set-up

Sztrókay, A; Herzen, Julia; Auweter, Sigrid; Liebhardt, Susanne; Mayr, Doris; Willner, Marian; Hahn, D; Zanette, Irène; Weitkamp, Timm; Hellerhoff, Karin; Pfeiffer, Franz; Reiser, Maximilian; Bamberg, Fabian

doi:10.1007/s00330-012-2592-1

Cited by 47 publications

(31 citation statements)

References 24 publications

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“…Thus, PCI can clearly define weakly X-ray-absorptive biological soft tissues without the use of a contrast agent. Several PCI modalities have been developed, such as interferometry [10], diffraction enhanced imaging (DEI) [11, 12], grating-based phase-contrast X-ray imaging (GB-PCI) [13], and in-line phase contrast imaging (IL-PCI) [14, 15]. Among them, IL-PCI has no requirements of superior temporal coherence within the X-ray source and complex experimental apparatus in the light path.…”

Section: Introductionmentioning

confidence: 99%

Neovascularization of hepatocellular carcinoma in a nude mouse orthotopic liver cancer model: a morphological study using X-ray in-line phase-contrast imaging

Zhang

et al. 2017

BMC Cancer

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BackgroundThis study aimed to determine whether synchrotron radiation (SR)-based X-ray in-line phase-contrast imaging (IL-PCI) can be used to investigate the morphological characteristics of tumor neovascularization in a liver xenograft animal model.MethodsA human hepatocellular carcinoma HCCLM3 xenograft model was established in nude mice. Xenografts were sampled each week for 4 weeks and fixed to analyze tissue characteristics and neovascularization using SR-based X-ray in-line phase contrast computed tomography (IL-XPCT) without any contrast agent.ResultsThe effect of the energy level and object–to-detector distance on phase-contrast difference was in good agreement with the theory of IL-PCI. Boundaries between the tumor and adjacent normal tissues at week 1 were clearly observed in two-dimensional phase contrast projection imaging. A quantitative contrast difference was observed from weeks 1 to 4. Moreover, 3D image reconstruction of hepatocellular carcinoma (HCC) samples showed blood vessels inside the tumor were abnormal. The smallest blood vessels measured approximately 20 μm in diameter. The tumor vascular density initially increased and then decreased gradually over time. The maximum tumor vascular density was 4.29% at week 2.ConclusionIL-XPCT successfully acquired images of neovascularization in HCC xenografts in nude mice.

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Section: Introductionmentioning

confidence: 99%

Neovascularization of hepatocellular carcinoma in a nude mouse orthotopic liver cancer model: a morphological study using X-ray in-line phase-contrast imaging

Zhang

et al. 2017

BMC Cancer

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“…First trials on ex vivo phase-contrast CT (PC-CT) imaging of breast samples showed greatly improved soft-tissue contrast and differentiability of fine structures compared to absorption-based imaging [15]–[17]. Microscopic structures like the walls of dilated ducts containing ductal carcinoma in situ can be visualized in PC-CT but not in absorption CT, an information that might be used for future differentiation between invasive and noninvasive carcinoma [18].…”

Section: Introductionmentioning

confidence: 99%

Visualizing Typical Features of Breast Fibroadenomas Using Phase-Contrast CT: An Ex-Vivo Study

Grandl¹,

Willner

Herzen

et al. 2014

PLoS ONE

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BackgroundFibroadenoma is the most common benign solid breast lesion type and a very common cause for histologic assessment. To justify a conservative therapy, a highly specific discrimination between fibroadenomas and other breast lesions is crucial. Phase-contrast imaging offers improved soft-tissue contrast and differentiability of fine structures combined with the potential of 3-dimensional imaging. In this study we assessed the potential of grating-based phase-contrast CT imaging for visualizing diagnostically relevant features of fibroadenomas.Materials and MethodsGrating-based phase-contrast CT was performed on six ex-vivo formalin-fixed breast specimens containing a fibroadenoma and three samples containing benign changes that resemble fibroadenomas using Talbot Lau interferometry and a polychromatic X-ray source. Phase-contrast and simultaneously acquired absorption-based 3D-datasets were manually matched with corresponding histological slices. The visibility of diagnostically valuable features was assessed in comparison with histology as the gold-standard.ResultsIn all cases, matching of grating-based phase-contrast CT images and histology was successfully completed. Grating-based phase-contrast CT showed greatly improved differentiation of fine structures and provided accurate depiction of strands of fibrous tissue within the fibroadenomas as well as of the diagnostically valuable dilated, branched ductuli of the fibroadenomas. A clear demarcation of tumor boundaries in all cases was provided by phase- but not absorption-contrast CT.ConclusionsPending successful translation of the technology to a clinical setting and considerable reduction of the required dose, the data presented here suggest that grating-based phase-contrast CT may be used as a supplementary non-invasive diagnostic tool in breast diagnostics. Phase-contrast CT may thus contribute to the reduction of false positive findings and reduce the recall and core biopsy rate in population-based screening. Phase-contrast CT may further be used to assist during histopathological workup, offering a 3D view of the tumor and helping to identify diagnostically valuable tissue sections within large tumors.

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“…Therefore, it has great potential e.g. in medical diagnostics for the detection of breast cancer [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Fast one-dimensional wave-front propagation for x-ray differential phase-contrast imaging

Wolf¹,

Malecki²,

Sperl³

et al. 2014

Biomed. Opt. Express

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Abstract:Numerical wave-optical simulations of X-ray differential phase-contrast imaging using grating interferometry require the oversampling of gratings and object structures in the range of few micrometers. Consequently, fields of view of few millimeters already use large amounts of a computer's main memory to store the propagating wave front, limiting the scope of the investigations to only small-scale problems. In this study, we apply an approximation to the Fresnel-Kirchhoff diffraction theory to overcome these restrictions by dividing the two-dimensional wave front up into 1D lines, which are processed separately. The approach enables simulations with samples of clinically relevant dimensions by significantly reducing the memory footprint and the execution time and, thus, allows the qualitative comparison of different setup configurations. We analyze advantages as well as limitations and present the simulation of a virtual mammography phantom of several centimeters of size. Reiser, and F. Bamberg, "Assessment of grating-based X-ray phase-contrast CT for differentiation of invasive ductal carcinoma and ductal carcinoma in situ in an experimental ex vivo set-up." Eur. Radiol. 2, 381-387 (2012). 9. F. Pfeiffer, M. Bech, O. Bunk, P. Kraft, E. F. Eikenberry, C. Bronnimann, C. Grünzweig, and C. David, "Hard-xray dark-field imaging using a grating interferometer," Nat. Mater. 7, 134-137 (2008

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Assessment of grating-based X-ray phase-contrast CT for differentiation of invasive ductal carcinoma and ductal carcinoma in situ in an experimental ex vivo set-up

Abstract: Our results indicate that unlike conventional CT, grating-based PC-CT may allow the differentiation between invasive carcinoma and intraductal carcinoma and healthy breast tissue and provide 3D visualisation of DCIS.

Cited by 47 publications

References 24 publications

Neovascularization of hepatocellular carcinoma in a nude mouse orthotopic liver cancer model: a morphological study using X-ray in-line phase-contrast imaging

Neovascularization of hepatocellular carcinoma in a nude mouse orthotopic liver cancer model: a morphological study using X-ray in-line phase-contrast imaging

Visualizing Typical Features of Breast Fibroadenomas Using Phase-Contrast CT: An Ex-Vivo Study

Fast one-dimensional wave-front propagation for x-ray differential phase-contrast imaging

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