Assessment of glycaemic control in non-insulin-dependent diabetes mellitus

Abstract

Blood glucose profiles, fasting blood glucose and glycated haemoglobin were followed prospectively for 6 weeks in 14 patients with non-insulin-dependent diabetes (NIDDM). Capillary blood glucose was sampled weekly on filter paper and analysed in the laboratory. Glycated haemoglobin was determined every other week with both cation-exchange chromatography (HbA1-IEC) and aminophenylboronic acid affinity chromatography (GHb-PBA). The mean for the blood glucose profiles was 11.2 +/- 3.5 mmol l-1 (+/- SD) and the mean for fasting blood glucose was 9.5 +/- 2.8 mmol l-1. For individual patients the differences between mean fasting blood glucose and mean blood glucose varied from -1.3 to 4.2 mmol l-1. There was a linear relationship between blood glucose and glycated haemoglobin which was steeper for GHb-PBA than for HbA1-IEC. The correlations with fasting blood glucose and mean blood glucose for HbA1-IEC were r = 0.85 and r = 0.92 and for GHb-PBA r = 0.87 and r = 0.96 respectively. Mean blood glucose was better correlated to glycated haemoglobin than fasting blood glucose (p < 0.05 for GHb-PBA).

Hyperglycemia and nocturnal systolic blood pressure are associatedwith left ventricular hypertrophy and diastolic dysfunction in hypertensive diabetic patients

“…Patients with type 2 DM are more stable in blood glucose control than patients with type 1 DM [ 23 ] and FBG has been commonly used to monitor glycemic control in type 2 diabetic patients treated with diet alone or oral hypoglycemic agents [ 24 ]. In both cross-sectional [ 25 - 27 ] and prospective [ 28 ] studies a good correlation was shown between FBG and HbA1. In addition, the retrospective average of FBG values is considered a good index to establish a previous long-term glycemic control in patients with type 2 DM [ 29 ].…”

Metal Complexes as Allosteric Effectors of Human Hemoglobin: An NMR Study of the Interaction of the Gadolinium(III) Bis(m-boroxyphenylamide)diethylenetriaminepentaacetic Acid Complex with Human Oxygenated and Deoxygenated Hemoglobin

“…Oxygenated unglycated human adult hemoglobin (HbA 0 ) and oxygenated glycated human adult hemoglobin (HbA 1c ) were obtained from chromatographic separation of HbA by means of aminophenylboronic acid affinity gel chromatography (GHb-PBA), where agarose-bound boronate groups reversibly bind glucose as syn-diols (Wettre et al, 1993). By this procedure HbA 0 was eluted first by using 50 mM ammonia buffer at pH 9.25 as the eluent.…”

Long-Term Glycemic Control Relates to Mortality in Type II Diabetes

“…Therefore, we decided not to use these data. However, FBG is known to relate well to values of glycosylated hemoglobin in type II diabetic subjects (30).…”