Assessment of glufosinate-containing herbicide exposure: A multi-center retrospective study

Hsiao, Jen-Tso; Pan, Hsiu-Yung; Kung, Chia-Te; Cheng, Fu-Jen; Chuang, Po-Chun

doi:10.1016/j.ajem.2021.08.017

“…2‐oxo‐4‐[(hydroxy)(methyl)phosphinoyl]butyric acid (PPO) is a valuable keto acid widely used as a precursor for the asymmetric synthesis of l ‐phosphinothricin ( l ‐PPT). l ‐phosphinothricin is the active ingredient of the nonselective, broad‐spectrum herbicide d , l ‐phosphinothricin ( d , l ‐PPT), and is widely used for postemergence weed control in agricultural and nonagricultural lands worldwide (Busi et al, 2018; Hsiao et al, 2021; Yan et al, 2022). In the current chemical synthesis route of PPT, the cost of producing the pure l ‐isomer is still significantly higher than that of the racemic mixture (Takano & Dayan, 2020).…”

Section: Introductionmentioning

confidence: 99%

Development of an aminotransferase‐driven biocatalytic cascade for deracemization of d,l‐phosphinothricin

Liu

¹

,

Wu

²

,

Deng

³

et al. 2023

Biotech & Bioengineering

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Abstract2‐oxo‐4‐[(hydroxy)(methyl)phosphinoyl]butyric acid (PPO) is the essential precursor keto acid for the asymmetric biosynthesis of herbicide l‐phosphinothricin (l‐PPT). Developing a biocatalytic cascade for PPO production with high efficiency and low cost is highly desired. Herein, a d‐amino acid aminotransferase from Bacillus sp. YM‐1 (Ym DAAT) with high activity (48.95 U/mg) and affinity (Km = 27.49 mM) toward d‐PPT was evaluated. To circumvent the inhibition of by‐product d‐glutamate (d‐Glu), an amino acceptor (α‐ketoglutarate) regeneration cascade was constructed as a recombinant Escherichia coli (E. coli D), by coupling Ym d‐AAT, d‐aspartate oxidase from Thermomyces dupontii (TdDDO) and catalase from Geobacillus sp. CHB1. Moreover, the regulation of the ribosome binding site was employed to overcome the limiting step of expression toxic protein TdDDO in E. coli BL21(DE3). The aminotransferase‐driven whole‐cell biocatalytic cascade (E. coli D) showed superior catalytic efficiency for the synthesis of PPO from d,l‐phosphinothricin (d,l‐PPT). It revealed the production of PPO exhibited high space–time yield (2.59 g L−1 h−1) with complete conversion of d‐PPT to PPO at high substrate concentration (600 mM d,l‐PPT) in 1.5 L reaction system. This study first provides the synthesis of PPO from d,l‐PPT employing an aminotransferase‐driven biocatalytic cascade.

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“…Although previous study [21] have found signi cant correlations between ammonia levels in the serum and CSF and neurological complications, CSF ammonia detection was not available in our hospitals include our hospital. Age > 70 years and GCS score < 9 at admission were predictors of mortality in patients with GA poisoning, according to Hsiao JT et al [22]. However, there was no signi cant difference in age, PCO2 level, or GCS score compared to published research, which may be explained by the formulation of GA herbicides differing in different countries [14].…”

Section: Discussionmentioning

confidence: 94%

Clinical features and prognostic factors of acute glufosinate ammonium

Huang

¹

,

Xie

²

,

Shen

³

et al. 2023

Preprint

0

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This retrospective study was conducted on 67 patients who suffered from acute oral glufosinate ammonium poisoning to summarize a comprehensive overview of the clinical characteristics. General clinical data and laboratory test results were collected from the time of admission to hospital discharge. The most common clinical symptom of GA poisoning were nausea and vomiting, The incidence of unfavorable outcomes and the mortality of GA poisoning were 41.8% and 13.4%. The unfavorable outcomes group had a higher initial serum ammonia level (75.7±17.3umol/L vs 47.5±12.8umol/L), more ingestion amount of GA (103.6±63.4ml vs 72.3±37.7ml) and higher-level CK-MB (43.5 U/L vs 16.0 U/L) compared to favorable outcomes groups. The area under the curve in the ROC analysis for the predictive variables, initial ammonia and CK-MB, were 0.873 and 0.680.The patients with GA poisoning may exhibit clinical symptoms of gastrointestinal and neurological toxicity, as well as respiratory failure which experience different latent period that we need to pay special attention to. Initial serum and CK-MB might be the most accurate prognostic factors for predicting the unfavorable outcomes.

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“…Although previous study [21] have found signi cant correlations between ammonia levels in the serum and CSF and neurological complications, CSF ammonia detection was not available in our hospitals include our hospital. Age > 70 years and GCS score < 9 at admission were predictors of mortality in patients with GA poisoning, according to Hsiao JT et al [22]. Lee [23] also found that PCO2 level, mechanical ventilator application, and the use of vasopressors could be valuable prognostic information for evaluating the severity and mortality of patients with GA poisoning.…”

Section: Discussionmentioning

confidence: 96%

Clinical features of acute glufosinate ammonium and initial serum ammonia and CK-MB as early predictors of unfavorable prognosis

Xue,

Xie,

Shen

et al. 2024

Preprint

0

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This retrospective study was conducted on 67 patients who suffered from acute oral glufosinate ammonium poisoning to summarize a comprehensive overview of the clinical characteristics. General clinical data and laboratory test results were collected from the time of admission to hospital discharge. The most common clinical symptom of GA poisoning were nausea and vomiting, The incidence of unfavorable outcomes and the mortality of GA poisoning were 41.8% and 13.4%. The unfavorable outcomes group had a higher initial serum ammonia level (75.7±17.3umol/L vs 47.5±12.8umol/L, P<0.001), more ingestion amount of GA (103.6±63.4ml vs 72.3±37.7ml, P=0.032) and higher-level CK-MB (43.5 U/L vs 16.0 U/L, P=0.002) compared to favorable outcomes groups. Multiple logistic regression analyses showed the initial serum ammonia (OR=1.031, 95%CI, 1.069-1.197, P<0.001)，ingestion amount of GA (OR=1.017, 95%CI, 0.997-1.039, P=0.013) and CK-MB (OR=1.017, 95%CI, 0.997-1.039, P=0.013) levels were independently associated with unfavorable outcomes. Receive operator characteristic (ROC) curves were used to determine the optimal initial serum ammonia level (62.7mmol/L with sensitivity of 85.7% and specificity of 87.2%, and AUC=0.873, 95%CI, 0.773-0.974, P<0.001), CK-MB level (43.5U/L with sensitivity of 42.9% and specificity of 87.2%, and AUC=0.680, 95%CI, 0.562-0.809, P=0.012) and the ingestion amount(110ml with sensitivity of 32.1% and specificity of 92.3%, and AUC=0.652, 95%CI, 0.518-0.786, P=0.035) respectively.The patients with GA poisoning may exhibit clinical symptoms of gastrointestinal and neurological toxicity, as well as respiratory failure after latent period that we need to pay special attention to prepare. Initial serum and CK-MB might be better accurate prognostic factors for predicting the unfavorable outcomes.

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Assessment of glufosinate-containing herbicide exposure: A multi-center retrospective study

Cited by 12 publications

References 26 publications

Development of an aminotransferase‐driven biocatalytic cascade for deracemization of d,l‐phosphinothricin

Development of an aminotransferase‐driven biocatalytic cascade for deracemization of d,l‐phosphinothricin

Clinical features and prognostic factors of acute glufosinate ammonium

Clinical features of acute glufosinate ammonium and initial serum ammonia and CK-MB as early predictors of unfavorable prognosis

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