Assessment of glucose levels in cyst fluid aspirates to evaluate cellular metabolism

Chatterjee, Shailja; Karadwal, Asha

doi:10.4103/srmjrds.srmjrds_41_17

Cited by 2 publications

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“…The mean glucose values were found to be highest in radicular and odontogenic keratocysts explaining their inflammatory and neoplastic natures, respectively. Residual cyst exhibited least glucose content indicating minimal metabolic activity 14 . It is hence surprising as to how some residual cysts attain massive sizes and cause severe destruction 6 …”

Section: Discussionmentioning

confidence: 99%

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Residual radicular cyst: A remnant or a distinct entity? Reigniting the debate

et al. 2021

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According to the World Health Organization's classification given in 1992, the odontogenic cysts associated with inflammation are: radicular cyst, residual cyst and paradental cyst. 1 Radicular cysts (RCs) are the most common inflammatory odontogenic cysts and arise from the epithelial residues in the periodontal ligament known as the cell rests of Malassez, which undergo inflammation following pulpal necrosis. 2 Residual radicular cysts (RRCs) also called as residual cysts, represent 10-18% of inflammatory odontogenic cysts and commonly present between the 4th-6th decade of life. 1,3 Maxilla is more commonly involved (66.2%), predominantly the posterior areas. Males are more commonly affected in a 3:2 ratio. 3 RRCs are believed to originate from a RC that is inadvertently left behind entirely or in fragments, following extraction of the involved tooth. 1 However, the identity of these cysts is still debated as few authors believe that majority of them represent slowly resolving RCs. 4 Compared to other odontogenic cysts, the attention paid to the RRCs within literature is significantly less. 5 Although these cysts are slow growing and are usually found incidentally, they have a potential to reach massive sizes, displace adjacent structures and transform into benign or malignant tumours. 6 This article reviews the literature available on the etiopathogenesis, presentation, management and prognosis of RRCs along with a report of a large residual cyst in right maxilla, displacing the maxillary sinus. The cyst was filled with brownish red viscous content which is uncommon. The lesion was enucleated and no post-operative complications were observed.

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Section: Discussionmentioning

confidence: 99%

“…Residual cyst exhibited least glucose content indicating minimal metabolic activity. 14 It is hence surprising as to how some residual cysts attain massive sizes and cause severe destruction. 6 Muglali et al (2008), evaluated the cytokine and chemokine levels in RC and RRC fluids.…”

Section: Analysis Of Cystic Fluidmentioning

confidence: 99%

Residual radicular cyst: A remnant or a distinct entity? Reigniting the debate

et al. 2021

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Comparative Metabolomics Reveals the Microenvironment of Common T-Helper Cells and Differential Immune Cells Linked to Unique Periapical Lesions

et al. 2021

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Periapical abscesses, radicular cysts, and periapical granulomas are the most frequently identified pathological lesions in the alveolar bone. While little is known about the initiation and progression of these conditions, the metabolic environment and the related immunological behaviors were examined for the first time to model the development of each pathological condition. Metabolites were extracted from each lesion and profiled using gas chromatography-mass spectrometry in comparison with healthy pulp tissue. The metabolites were clustered and linked to their related immune cell fractions. Clusters I and J in the periapical abscess upregulated the expression of MMP-9, IL-8, CYP4F3, and VEGF, while clusters L and M were related to lipophagy and apoptosis in radicular cyst, and cluster P in periapical granuloma, which contains L-(+)-lactic acid and ethylene glycol, was related to granuloma formation. Oleic acid, 17-octadecynoic acid, 1-nonadecene, and L-(+)-lactic acid were significantly the highest unique metabolites in healthy pulp tissue, periapical abscess, radicular cyst, and periapical granuloma, respectively. The correlated enriched metabolic pathways were identified, and the related active genes were predicted. Glutamatergic synapse (16–20),-hydroxyeicosatetraenoic acids, lipophagy, and retinoid X receptor coupled with vitamin D receptor were the most significantly enriched pathways in healthy control, abscess, cyst, and granuloma, respectively. Compared with the healthy control, significant upregulation in the gene expression of CYP4F3, VEGF, IL-8, TLR2 (P < 0.0001), and MMP-9 (P < 0.001) was found in the abscesses. While IL-12A was significantly upregulated in cysts (P < 0.01), IL-17A represents the highest significantly upregulated gene in granulomas (P < 0.0001). From the predicted active genes, CIBERSORT suggested the presence of natural killer cells, dendritic cells, pro-inflammatory M1 macrophages, and anti-inflammatory M2 macrophages in different proportions. In addition, the single nucleotide polymorphisms related to IL-10, IL-12A, and IL-17D genes were shown to be associated with periapical lesions and other oral lesions. Collectively, the unique metabolism and related immune response shape up an environment that initiates and maintains the existence and progression of these oral lesions, suggesting an important role in diagnosis and effective targeted therapy.

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Assessment of glucose levels in cyst fluid aspirates to evaluate cellular metabolism

Cited by 2 publications

References 0 publications

Residual radicular cyst: A remnant or a distinct entity? Reigniting the debate

Residual radicular cyst: A remnant or a distinct entity? Reigniting the debate

Comparative Metabolomics Reveals the Microenvironment of Common T-Helper Cells and Differential Immune Cells Linked to Unique Periapical Lesions

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