Assessment of genotoxic effects of chloropyriphos and acephate by the comet assay in mice leucocytes

Rahman, M. F.; Mahboob, M.; Danadevi, K.; Banu, Bilquis; Grover, Paramjit

doi:10.1016/s1383-5718(02)00033-5

Cited by 130 publications

(72 citation statements)

References 40 publications

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“…Varios autores informaron que esta sustancia causa daño genético in vivo e in vitro porque mostró resultados positivos al evaluarla con diferentes sistemas, como los ensayos de aberraciones cromosómicas, de micronúcleos, de mutagénesis e incluso con el ensayo cometa. Sin embargo, su potencial mutagénico y carcinogénico continúa siendo controversial (Stachetti et al 1998, Rahman et al 2002.…”

Section: Discussionunclassified

Genotoxicidad de tres plaguicidas utilizados en la actividad bananera de Costa Rica

Vindas

Ortíz

Ramírez

et al. 2014

RBT

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Genotoxicity of three pesticides used in Costa Rican banana plantations. The in vitro genotoxicity of imazalil and thiabendazole fungicides and the insecticide chlorpyrifos, compounds used in Costa Rican banana plantations, was evaluated with the single-cell gel electrophoresis technique (comet assay). The comet assay is a simple, rapid and low cost technique for quantification of DNA damage. This assay detects DNA single-strand breaks and alkali-labile sites in individual cells. The effects were analyzed by using human lymphocytes exposed to doses of 0, 25, 50, 75 and 100 µg/ml of each pesticide for 30 min at 37°C. The cells were embedded in agarose, lysed, subjected to alkaline electrophoresis (pH> 13) for 20 min at 25V, neutralized and dehydrated to be stained with a fluorescent dye and later comets visualization with the epifluorescence microscope. Chlorpyrifos and imazalil induced significant DNA damage in a dose-dependent manner. Chlorpyrifos was the major inductor of DNA breaks. These results indicate that both are genotoxic compounds in vitro. Thiabendazole fungicide did not induced DNA damage using the comet assay for all concentrations tested. Rev. Biol. Trop. 52(3): 601-609. Epub 2004 Dic 15.

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Section: Discussionunclassified

Genotoxicidad de tres plaguicidas utilizados en la actividad bananera de Costa Rica

Vindas

Ortíz

Ramírez

et al. 2014

RBT

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“…This type of mutagenesis assay detects the ability of compounds to interact with alkali-labile DNA sites and induce DNA strand breaks. Rahman et al (2002) treated mice orally with single doses of chlorpyrifos ranging from 0.28 to 8.96 mg/kg. In blood leukocytes dose-dependent increases of comet tail length were seen after 24 h. These changes decreased after 48 and 72 h and disappeared after 96 h. The authors discuss formation of reactive oxygen species (ROS) as a possible mechanism responsible for DNA damage, as numerous studies have shown that chlorpyrifos increases indicators of oxidative stress or leads to oxidative damage to various tissues or cells (Oncu et al, 2002;Verma and Srivastava, 2003;Wielgomas and Krechniak, 2007).…”

Section: Iiib1 Mutagenicitymentioning

confidence: 99%

Review of the Toxicology of Chlorpyrifos With an Emphasis on Human Exposure and Neurodevelopment

Eaton¹,

Daroff²,

Autrup³

et al. 2008

Critical Reviews in Toxicology

567

413

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“…On the other hand, Carver et al (1985) showed that acephate did not induce CAs, SCE or MN in mouse bone marrow cells. After the treatment with acephate, Rahman et al (2002) observed a significant increase in mean comet tail length indicating DNA damage in vivo in mouse lymphocytes in comparison to control. The damage was concentration related.…”

Section: Discussionmentioning

confidence: 86%

“…Chromosomal aberrations, SCE and micronucleus analysis of human lymphocytes as well as single cell gel electrophoresis (SCGE) or ''comet'' assay are used as the most rapid, sensitive and useful assays to detect the potential genotoxicity of chemicals (Rahman et al 2002;Ç elik et al 2004;Yüzbaşıoglu et al 2006Yüzbaşıoglu et al , 2008Yılmaz et al 2008). They have been considered to be the markers of early biological effects of carcinogen exposure (Liou et al 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Among the pesticides, organophosphorous insecticides are the most widely used synthetic chemicals for the control of agricultural and domestic insects. The use of this class of chemicals has resulted in worldwide increases in food and fiber production, the control of major disease carrying vectors and structure damaging insect pest (Chen et al 1999;Rahman et al 2002). However, in addition to their intended effects, they are sometimes found to affect non-target organisms, including humans (ChantelliForti et al 1993;Chaudhuri et al 1999;Karabay and Oguz 2005).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the cytogenetic damage induced by the organophosphorous insecticide acephate

et al. 2009

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The organophosphorous insecticide acephate was tested for its ability to induce in vitro cytogenetic effect in human peripheral lymphocytes by using the chromosomal aberrations (CAs), sister chromatid exchange (SCE) and micronuclei (MN) assay. The level of nuclear DNA damage of acephate was evaluated by using the comet assay. Concentrations of 12.5, 25, 50, 100 and 200 lg mL -1 of acephate were used. All concentrations of acephate induced significant increase in the frequency of CAs and in the formation of MN dose dependently (r = 0.92 at 24 h, r = 0.95 at 48 h for CAs, r = 0.87 for MN). A significant increase was observed in induction of SCE at 50, 100 and 200 lg mL -1 concentrations during 24 h treatment and at all concentrations (except 12.5 lg mL -1 ) during 48 h treatment period in a dose-dependent manner (r = 0.84 at 24 h, r = 0.88 at 48 h). Acephate did not affect the replicative index and cytokinesis-block proliferation index (CBPI). However, it significantly decreased the mitotic index at all three highest concentrations (50, 100, 200 lg mL -1 ) for 24 h treatment and at all concentrations (except 12.5 lg mL -1 ) for 48 h treatment, dose-dependently (r = 0.94 at 24 h, r = 0.92 at 48 h). A significant increase in mean comet tail length was observed at 100 and 200 lg mL -1 concentrations compared with negative control in a concentration-dependent manner (r = 0.94). The mean comet tail intensity was significantly increased at only 200 lg mL -1 concentration. The present results indicate that acephate is a clastogenic, cytotoxic agent and it causes DNA damage at high concentrations in human lymphocytes in culture.

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Assessment of genotoxic effects of chloropyriphos and acephate by the comet assay in mice leucocytes

Cited by 130 publications

References 40 publications

Genotoxicidad de tres plaguicidas utilizados en la actividad bananera de Costa Rica

Genotoxicidad de tres plaguicidas utilizados en la actividad bananera de Costa Rica

Review of the Toxicology of Chlorpyrifos With an Emphasis on Human Exposure and Neurodevelopment

Evaluation of the cytogenetic damage induced by the organophosphorous insecticide acephate

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