Assessment of genotoxic and mutagenic effects of chlorpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis

Ali, Daoud; Nagpure, N. S.; Kumar, Sudhir; Kumar, Ravindra; Kushwaha, Basdeo; Lakra, W.S.

doi:10.1016/j.fct.2008.12.021

Cited by 156 publications

(94 citation statements)

References 50 publications

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“…Author(s) agree that this article remains permanently open access under the terms of the Creative Commons Attribution License 4.0 International License pesticide widely used throughout the world for agriculture and domestic purposes (Ali et al, 2009). Pesticides at high concentrations are known to reduce the survival, growth, and reproduction of fish and produce many visible effects on fish (Rahman et al, 2002).…”

Section: Introductionmentioning

confidence: 94%

Histopathological effects of Cyperdicot and vitamin E supplementation on selected organs of Clarias gariepinus (Burchell, 1822) reared in a tropical fish farm in Nigeria

Odo¹,

Agwu²,

Madu³

et al. 2016

Afr. J. Biotechnol.

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Section: Introductionmentioning

confidence: 94%

Histopathological effects of Cyperdicot and vitamin E supplementation on selected organs of Clarias gariepinus (Burchell, 1822) reared in a tropical fish farm in Nigeria

Odo¹,

Agwu²,

Madu³

et al. 2016

Afr. J. Biotechnol.

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“…Fish were stocked in large aquarium tanks disinfected with potassium permanganate to prevent fungal infection and washed thoroughly prior to introduction of fish (Ali et al, 2009). The specimens were given prophylactic treatment by bathing them twice in 0.05% potassium permanganate (KMnO 4 ) solution for 2 min to avoid any dermal infections.…”

Section: Experimental Fish and Chemicalsmentioning

confidence: 99%

<i>IN-VIVO</i> EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN <i>CATLA CATLA</i>

Arunachalam

Annamalai

Kuruva

2013

American Journal of Environmental Sciences

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In the present study, the acute toxicity of Chromium in fingerlings of Catla catla, an Indian major carp, was evaluated with renewal bioassay method. In vivo studies were designed to assess the extent of Micronucleus Assay, Comet Assay under the exposure of common heavy-metal compounds, namely, Chromium Nitrate, using Catla catla (2n = 20), as a test model. The laboratory acclimatized fishes were divided into four groups. Group I served as positive control and the other three as exposed groups for three different time durations of 7, 14 and 21 days and were subjected to uninterrupted sub lethal concentrations (50% of 96 h LC 50 ). The experiments were planned in such a way that fish from all the groups were sacrificed on the same day. The frequencies of micronuclei and bi-nuclei were evaluated comparatively in peripheral erythrocytes. As a result, it was observed that, the fishes and different tissues showed differential sensitivity to the heavy-metal treatment. A significant increase in the frequencies of micronucleated and binucleated cells and percentage increase in DNA tail (p<0.001) through Alkaline Comet Assay were observed after 21 days of exposure to chromium. Our results also showed decrease in enzyme Superoxide Dismutase (SOD) activity and increase in catalase enzyme due to increasing chromium concentration. The bio-concentration factor profiles of Chromium in Catla catla during sub lethal toxicity study was also calculated.

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“…Numerous environmental issues have arisen so far due to the excessive use of this chemical compound (3), as it, among other consequences, causes toxic effects in non-target aquatic organisms, especially fish (4). Fish are used to assess the health of aquatic environments and physiological changes occurring as a result of pollution and multiple studies have already established that chlorpyrifos has various detrimental effects on t h e m ( 5 -1 0 ) , s u c h a s n e u r o t o x i c i t y v i a acetylcholinesterase inhibition (11), biochemical and histopathological alterations (12, 13), oxidative stress (12, 14), genotoxicity (15), and olfactory and neurobehavioral injuries (16). It therefore poses a serious threat to aquatic organisms as well as to human health (17).…”

mentioning

confidence: 99%

In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout

Topal¹,

Atamanalp²,

Oruç³

et al. 2014

Archives of Industrial Hygiene and Toxicology

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Chlorpyrifos is an organophosphate pesticide widely used in agriculture and aquaculture. This study investigated its effects on carbonic anhydrase (CA) enzyme activity and histopathology of rainbow trout gill and liver. The fish were exposed to 2.25 (25 % of 96 h LC 50 ), 4.5 (50 % of 96 h LC 50 ), and 6.75 µg L -1 (75 % of 96 h LC 50 ) of chlorpyrifos for 24, 48, 72, and 96 h. CA activity was measured in liver and gills and histopathological changes were examined by light microscopy. The most common liver changes at most of the chlorpyrifos concentrations were hyperaemia and degenerative changes. Gill tissues were characterised by lamellar hyperaemia, lamellar oedemas, clumping, cellular degeneration, hyperplasia, and lamellar atrophy. CA enzyme activity in the gills decreased at all concentrations at 48, 72, and 96 h after exposure to chlorpyrifos (p<0.05). Similarly, there was a time-dependent decrease in CA activity at all of the concentrations in liver tissues (p<0.05). The present study indicated that chlorpyrifos inhibits CA enzyme activity and causes histopathological damage in gill and liver tissues.

show abstract

Assessment of genotoxic and mutagenic effects of chlorpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis

Cited by 156 publications

References 50 publications

Histopathological effects of Cyperdicot and vitamin E supplementation on selected organs of Clarias gariepinus (Burchell, 1822) reared in a tropical fish farm in Nigeria

Histopathological effects of Cyperdicot and vitamin E supplementation on selected organs of Clarias gariepinus (Burchell, 1822) reared in a tropical fish farm in Nigeria

<i>IN-VIVO</i> EVALUATION OF HEXAVALENT CHROMIUM INDUCED DNA DAMAGE BY ALKALINE COMET ASSAY AND OXIDATIVE STRESS IN <i>CATLA CATLA</i>

In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout

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