Assessment of genetic variations of Nile Tilapia (Oreochromis niloticus L.) in the Volta Lake of Ghana using microsatellite markers

Mireku, Kwadwo Kesse; Kassam, Daud; Changadeya, Wisdom; Attipoe, F.Y.K.; Adinortey, C. A.

doi:10.5897/ajb2016.15796

Cited by 12 publications

(15 citation statements)

References 23 publications

(27 reference statements)

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“…Gu et al (2014) found that observed heterozygosity (Ho = 0.4483) in six Oreochromis populations in the primary rivers of Guangdong province were lower than the expected heterozygosity (He = 0.7097). On the contrary, Mireku et al (2017) showed that observed heterozygosity (Ho = 0.526) of nine populations of O. niloticus in the Volta lake of Ghana was slightly higher than the expected heterozygosity (He = 0.459). In addition, Hassanien and Gilbey (2005) reported that the average of expected and observed heterozygosity were higher in O. niloticus populations from river Nile (He = 0.884 and Ho = 0.815) than from Delta lake populations (He = 0.846 and Ho = 0.533).…”

Section: Discussionmentioning

confidence: 80%

“…In addition, genetic variation is important for a population's adaptation capacity towards changing environmental conditions (Fischer et al, 2017). Mireku et al (2017) found higher genetic variation within populations than among populations in Nile tilapia populations from Lake Volta in Ghana. In this study AMOVA revealed the existence of higher genetic variation within populations than between populations.…”

Section: Discussionmentioning

confidence: 99%

“…As such, knowledge of population genetic structure and genetic diversity of O. niloticus is crucial both for conservation practices and for fish breeders. Previous studies examined the genetic structure and diversity between populations of Nile tilapia (Oreochromis niloticus), based either on phenotypic traits (Trewavas, 1983), allozymes (Sodsuk and McAndrew, 1991), mitochondrial DNA (Romana-Eguia et al, 2004), randomly amplified polymorphic DNA (Hassanien et al, 2004) or microsatellites (Bhassu et al, 2004;Hassanien and Gilbey, 2005;Mireku et al, 2017). However, the genetic markers used to date have limitations regarding their maximal resolution in detecting the complex genetic structure typically encountered in Nile tilapia populations.…”

Section: Introductionmentioning

confidence: 99%

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Population Structure and Genetic Diversity of Nile Tilapia (Oreochromis niloticus) Strains Cultured in Tanzania

et al. 2019

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Understanding population structure and genetic diversity within and between local Nile tilapia lines cultured in Tanzania is important for sustainable aquaculture production. This study investigated the genetic structure and diversity among seven Nile tilapia populations in Tanzania (Karanga, Igunga, Ruhila, Fisheries Education and Training Agency, Tanzania Fisheries Research Institute, Kunduchi, and Lake Victoria). Double-digest restriction site-associated DNA (ddRAD) libraries were prepared from 140 individual fish (20 per population) and sequenced using an Illumina HiSeq 4000 resulting in the identification of 2,180 informative single nucleotide polymorphisms (SNPs). Pairwise F st values revealed strong genetic differentiation between the closely related populations; FETA, Lake Victoria, and Igunga and those from TAFIRI and Karanga with values ranging between 0.45 and 0.55. Population structure was further evaluated using Bayesian model-based clustering (STRUCTURE) and discriminant analysis of principal components (DAPC). Admixture was detected among Karanga, Kunduchi, and Ruhila populations. A cross-validation approach (25% of individual fish from each population was considered of unknown origin) was conducted in order to test the efficiency of the SNP markers to correctly assign individual fish to the population of origin. The cross-validation procedure was repeated 10 times resulting in 77% of the tested individual fish being allocated to the correct population. Overall our results provide a new database of informative SNP markers for both conservation management and aquaculture activities of Nile tilapia strains in Tanzania.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Population Structure and Genetic Diversity of Nile Tilapia (Oreochromis niloticus) Strains Cultured in Tanzania

et al. 2019

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“…In Ghana, as in many other sub-Saharan African countries where O. niloticus is the main aquaculture species, locally available native strains are widely considered inferior to the GIFT and related strains with respect to growth performance. Recognition of the need for improved strains of tilapia in Africa and the desire to ensure that Africa benefits from the GIFT project without the associated ecological and genetic risks of introducing the GIFT strain resulted in the development of the Ghanaian Akosombo and the Egyptian Abbassa strains using the GIFT selective breeding methodology [9][10][11][12]. In recent years, however, many commercial farmers in Ghana have expressed discontentment with the growth and survival rates of the Akosombo strain.…”

Section: Introductionmentioning

confidence: 99%

“…Screening nine microsatellite DNA loci across 350 samples from ten natural populations, Bezault et al [15] found high genetic differentiation across the Ethiopian, Nilotic and Sudano-Sahelian regions and ichthyofaunal provinces, and intermediate levels of divergence between populations in rivers and lakes within regions, presumably reflecting relatively recent interruptions of gene flow between hydrographic basins. While some research has focused on the genetic variation among O. niloticus populations within the Volta system in West Africa [12,16,17], ours is the first study to focus upon genetic differentiation among O. niloticus populations for purposes of conservation planning for wild populations. The assessment of the genetic variability among natural populations of O. niloticus in aquaculture receiving waters will provide vital baseline information for purposes of conserving any genetically distinct native populations remaining and for ongoing monitoring of aquaculture impacts on aquatic ecosystems in Ghana.…”

Section: Introductionmentioning

confidence: 99%

Aquaculture-Mediated Invasion of the Genetically Improved Farmed Tilapia (Gift) into the Lower Volta Basin of Ghana

2019

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The need for improved aquaculture productivity has led to widespread pressure to introduce the Genetically Improved Farmed Tilapia (GIFT) strains of Nile tilapia (Oreochromis niloticus) into Africa. However, the physical and regulatory infrastructures for preventing the escape of farmed stocks into wild populations and ecosystems are generally lacking. This study characterized the genetic background of O. niloticus being farmed in Ghana and assessed the genetic effects of aquaculture on wild populations. We characterized O. niloticus collected in 2017 using mitochondrial and microsatellite DNA markers from 140 farmed individuals sampled from five major aquaculture facilities on the Volta Lake, and from 72 individuals sampled from the wild in the Lower Volta River downstream of the lake and the Black Volta tributary upstream of the lake. Our results revealed that two farms were culturing non-native O. niloticus stocks, which were distinct from the native Akosombo strain. The non-native tilapia stocks were identical to several GIFT strains, some of which showed introgression of mitochondrial DNA from non-native Oreochromis mossambicus. We also found that the non-native cultured tilapias have escaped into the wild and interbred with local populations, and also observed potentially admixed individuals on some farms. Our results highlight aquaculture as a vector in the spread of invasive non-native species and strains, and underscore the importance of genetic baseline studies to guide conservation planning for wild populations.

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Population structure and genetic diversity of Nile tilapia (Oreochromis niloticus) using microsatellite markers from selected water bodies in southwest Ethiopia

Ahmed,

Hordofa,

Meressa

et al. 2023

Veterinary Medicine & Sci

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BackgroundThe Ethiopian southwest drainage has been designated a huge potential for fishery and aquaculture development. However, the genetic diversity of Nile tilapia along the main water bodies has yet to be uncovered. Therefore, this study used microsatellite markers to assess the genetic structure and diversity of Nile cichlid (Oreochromis niloticus) populations.MethodsOne hundred and sixty‐seven fish were collected from Alwero Dam, Baro River, Gilgel Gibe I Dam and Gojeb River southwest of Ethiopia. Genomic DNA was extracted from 40 tissue samples using a Qiagen DNA tissue extraction kit and genetic polymorphism was estimated using GenAlEx6.502 software.ResultsThe result showed that four loci were polymorphic and produced 3–6 alleles per locus, while the number of effective alleles (Ne) and the number of distinct alleles (Na) were 1.370 and 2.063, respectively. Observed heterozygosity (Ho) and expected heterozygosity (He) were expanded between 0.100.50 and 0.115–0.265, respectively. Baro River population revealed the highest mean expected heterozygosity of 0.265, whereas the lowest expected mean heterozygosity of 0.115 was from the Alwero Dam. The genetic differentiation between populations (FST) resulted in a moderate to a very high degree of divergence between the populations. The Alwero Dam and Gojeb River populations were the most divergent populations (FST = 0.524), unlike those from the Alwero Dam and Baro River. The genetic variation among individuals within populations was 69%, whereas 31% appeared among populations and no variation within individuals to the overall variance.ConclusionThis study confirmed the presence of significant genetic variations and differentiation among populations except between Gilgel Gibe I Dam and Gojeb River. This study highlights the genetic structure and differentiation amongst wild Nile tilapia (O. niloticus) populations in south‐western Ethiopia and paves the way for selective aquaculture breeding programs in the study area.

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Assessment of genetic variations of Nile Tilapia (Oreochromis niloticus L.) in the Volta Lake of Ghana using microsatellite markers

Cited by 12 publications

References 23 publications

Population Structure and Genetic Diversity of Nile Tilapia (Oreochromis niloticus) Strains Cultured in Tanzania

Population Structure and Genetic Diversity of Nile Tilapia (Oreochromis niloticus) Strains Cultured in Tanzania

Aquaculture-Mediated Invasion of the Genetically Improved Farmed Tilapia (Gift) into the Lower Volta Basin of Ghana

Population structure and genetic diversity of Nile tilapia (Oreochromis niloticus) using microsatellite markers from selected water bodies in southwest Ethiopia

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