Assessment of Genetic Variations among Medicinal Plant Cassia tora from Different Geographic Regions of Central India Using RAPD Markers

Tilwari, Anita; Chauhan, Deepak Kumar; Sharma, Rajesh; Singh, R. P.

doi:10.4172/2167-0412.1000276

Cited by 10 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ecotypic diversity of S. tora in Central India was also reported by Tilwari et al (2016) although based on molecular data. They estimated the genetic diversity of 15 accessions of C. tora from different agro-climatic zones of Madhya Pradesh.…”

Section: Ecotypic Diversity Of In Situ Plantsmentioning

confidence: 57%

Underutilized Senna tora (L.) Roxb. - a highly potential multipurpose species for food, feed, medicine and climate resilience for the Philippines

Jamago¹,

Valleser²,

Galleron³

et al. 2018

CMUJS

View full text Add to dashboard Cite

show abstract

Section: Ecotypic Diversity Of In Situ Plantsmentioning

confidence: 57%

Underutilized Senna tora (L.) Roxb. - a highly potential multipurpose species for food, feed, medicine and climate resilience for the Philippines

Jamago¹,

Valleser²,

Galleron³

et al. 2018

CMUJS

View full text Add to dashboard Cite

show abstract

“…It is a well-known fact that the sustainability of any plant population depends on their genetic diversity. Moreover, factors such as population isolation, genetic drift, mating system, gene flow, selection, and shifts in distribution are responsible for alterations in complex genetic constituting and further cause variations in hereditary multiplicity within the population [26,27]. The position of the genotype from Soo hyang (SM6) in relation to other genotypes merits special mention, as this genotype was found to have an intermediate genetic relationship between four other genotypes within the major cluster.…”

Section: Discussionmentioning

confidence: 99%

Estimating Genetic Conformism of Korean Mulberry Cultivars Using Random Amplified Polymorphic DNA and Inter-Simple Sequence Repeat Profiling

Sheet

Ghosh

Acharya³

et al. 2018

Plants

View full text Add to dashboard Cite

Apart from being fed to silkworms in sericulture, the ecologically important Mulberry plant has been used for traditional medicine in Asian countries as well as in manufacturing wine, food, and beverages. Germplasm analysis among Mulberry cultivars originating from South Korea is crucial in the plant breeding program for cultivar development. Hence, the genetic deviations and relations among 8 Morus alba plants, and one Morus lhou plant, of different cultivars collected from South Korea were investigated using 10 random amplified polymorphic DNA (RAPD) and 10 inter-simple sequence repeat (ISSR) markers in the present study. The ISSR markers exhibited a higher polymorphism (63.42%) among mulberry genotypes in comparison to RAPD markers. Furthermore, the similarity coefficient was estimated for both markers and found to be varying between 0.183 and 0.814 for combined pooled data of ISSR and RAPD. The phenogram drawn using the UPGMA cluster method based on combined pooled data of RAPD and ISSR markers divided the nine mulberry genotypes into two divergent major groups and the two individual independent accessions. The distant relationship between Dae-Saug (SM1) and SangchonJo Sang Saeng (SM5) offers a possibility of utilizing them in mulberry cultivar improvement of Morus species of South Korea.

show abstract

“…With the development of various taxonomical tools that based on the molecular and metabolic traits, new taxonomical systems have been evolved, exploring new criteria for assessing the evolutionary status of the individual taxa in the subtribe Cassiinae. Recently, several molecular markers for demonstrating the intra, and interspecific genetic variations have been implemented for the direct comparison of the plant variation at biochemical and molecular levels [ 32 , 33 ]. Random amplified polymorphic DNA (RAPD) analysis and sequences of the internal transcribed spacer (ITS) are one of the recent molecular tools for separating the particular species from their ancestors.…”

Section: Introductionmentioning

confidence: 99%

Molecular and metabolic traits of some Egyptian species of Cassia L. and Senna Mill (Fabaceae-Caesalpinioideae)

et al. 2022

View full text Add to dashboard Cite

The genus Cassia and Senna have been classified under subfamily Caesalpinioideae of family Fabaceae (Leguminosae) of order Fabales. There is a scarce taxonomical studies of the genus Cassia and Senna inhabiting Egyptian environments, thus, the main objective of the current was to revise and authenticate the phylogenetic relationship between studied taxa of the species of the genera Cassia and Senna in Egypt using the recent tools of ITS barcoding, RAPD analysis and metabolic profiling, in comparing to the traditional taxonomical features. From the cluster analysis of the traditional 27 morphological characters, the studied taxa were categorized into two major clades with an average taxonomic distance of 4.3. The clade I include Cassia fistula, C. renigera, C. javanica L subsp. nodosa and C. roughiia that belongs to series Obolospermae, and C. grandis that belongs to series Grandes. The clade (II) includes Senna surattensis and S. alata at taxonomic level 3.6. The taxonomical description of the studied taxa was confirmed from the molecular analysis of ITS sequences and RAPD analysis. The ITS sequences of the tested plants species C. fistula L, C. grandis MD4, C. javanica subsp. nodosa MD7, C. roxburghii MD5, C. renigera MD5 were deposited at genbank with accession numbers MW367973, MZ960447, MW386305, MW326753 and MW32685, respectively. While, the ITS sequences of the S. surrattensis and S. alata were deposited into genbank accession # MD14 MW367670 and MD20 MW412635, respectively. Thus, from the molecular analysis, two clades were clearly separated into Clade I of Cassia and Clade II of Senna. The cluster I represented by C. fistula, C. renigera, C. roxburghii, and C. javanica sub nodosa, and the cluster II represented by S. alata and S. surattensis. From the PCA of RAPD, a clearly discrimination between the two Taxa was observed revealing the characteristic grouping of Cassia and Senna. The species Senna alata and Senna surattensis were grouped together, but the species of C. renigera, C. javanica, C. roxburghii and C. grandis was grouped on a distinct group. The separation of Cassia and Senna species into two clusters verify the segregation of the genus Cassia L. senso lato into two distinct genera namely Senna P. and Cassia L. The morphological, molecular traits of the studied plants were authenticated from the metabolic profiling by GC-MS analysis. Among the 23 identified metabolites, four compounds namely hexadecanoic acid, methyl ester, 9-Octadecenoic acid (Z)-ethyl ester and Vitamin E were detected with fluctuated concentrations, among C. fistula, C. grandis, C. javanica subsp. nodosa and C. roxburghii. Conclusively, the traditional morphological features, molecular barcoding using ITS sequences, RAPD analysis and metabolic traits by GC-MS analysis, authenticates the taxonomical diversity of the genus Cassia and Senna.

show abstract

Assessment of Genetic Variations among Medicinal Plant Cassia tora from Different Geographic Regions of Central India Using RAPD Markers

Cited by 10 publications

References 22 publications

Underutilized Senna tora (L.) Roxb. - a highly potential multipurpose species for food, feed, medicine and climate resilience for the Philippines

Underutilized Senna tora (L.) Roxb. - a highly potential multipurpose species for food, feed, medicine and climate resilience for the Philippines

Estimating Genetic Conformism of Korean Mulberry Cultivars Using Random Amplified Polymorphic DNA and Inter-Simple Sequence Repeat Profiling

Molecular and metabolic traits of some Egyptian species of Cassia L. and Senna Mill (Fabaceae-Caesalpinioideae)

Contact Info

Product

Resources

About