Assessment of genetic stability amongst micropropagated Ansellia africana, a vulnerable medicinal orchid species of Africa using SCoT markers

Bhattacharyya, Paromik; Kumar, Vijay; Staden, J. Van

doi:10.1016/j.sajb.2016.11.007

Cited by 72 publications

(21 citation statements)

References 55 publications

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“…Another problem found in plant tissue culture involves the genetic stability of the plants. Different cases of micropropagation of in vitro-regenerated plants have shown that they are not always clonal copies of the mother plant (Devi et al 2014;Bhattacharyya et al 2017a). In vitro culture conditions, especially some growth regulators and elicitors, act as stress factors that induce alterations in sensitive regions of plant genome, and therefore, generate instability in cultured cells, tissues and organs, an occurrence known as somaclonal variation (Larkin and Scowcroft 1981;Gyulai et al 2003;Bairu et al 2011;Stanišic et al 2015;Govindaraju and Arulselvi 2016).…”

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

“…Therefore, to screen somaclonal variability within a cell culture, it is necessary to monitor and assess the genetic constitution and stability of the in vitro-regenerated plants. The methodologies involved in that process include the use of several techniques to assess possible alterations at different levels (Devarumath et al 2002;Bhattacharyya et al 2015Bhattacharyya et al , 2017aBose et al 2016;Bhattacharyya and Van Staden 2016). Flow cytometry and chromosome counting are widely used to assess changes in ploidy and chromosome number; PCRbased DNA markers, random amplified polymorphic DNA (RAPD), random fragmented length polymorphism (RFLP), inter simple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), microsatellite markers, and start codon targeted (ScoT) polymorphism have been successfully used to evaluate genomic stability of regenerated plants (Hu et al 2008;Collard and Mackill 2009;Bairu et al 2011;Singh et al 2013;Bhattacharyya et al 2014Bhattacharyya et al , 2015Rathore et al 2014;Stanišic et al 2015;Bose et al 2016;Govindaraju and Arulselvi 2016).…”

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

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In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

417

206

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Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

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Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

417

206

View full text Add to dashboard Cite

show abstract

“…Molecular techniques are most commonly used to detect somaclonal variation, since they are efficient, unaffected by environmental factors, and the results are reproducible and trustworthy. Various conventional molecular markers, including random amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR), and simple sequence repeats (SSR) have been used extensively in the assessment of clonal fidelity (Bhattacharyya et al 2017). These have proven to be a polymorphic, rapid, and simple way to assess somaclonal variation.…”

Section: Introductionmentioning

confidence: 99%

Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

Chávez-Cortázar¹,

Mata-Rosas²,

Oyama³

et al. 2020

Biologia plant.

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The utility of plant tissue culture for the mass propagation of trees is well known, but continuous in vitro multiplication of plant material may increase the possibility of somaclonal variation; therefore, it is essential to evaluate the genetic integrity of regenerants from species-specific in vitro protocols prior to mass production and implementation. The objectives of this study were: 1) to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration over two cycles of secondary somatic embryogenesis in Magnolia dealbata; and 2) to verify the genetic stability of the regenerants obtained. The embryogenic response was not significantly affected by the concentration of 2,4-D but did vary across cycles of induction. The addition of 4.52 μM 2,4-D induced the highest total number of embryos (100.5), the mean number of somatic embryos (25.1) and somatic embryos per explant (80.6). In both 2,4-D concentration (2.26 or 4.52 μM), genetic integrity between the donor and the propagated clones was 0.90, and the low genetic instability (≤ 0.10 in both PGR treatments) might be due to effect of cyclic somatic embryogenesis or the different response of the explants at stress in in vitro culture conditions. However, it is necessary to examine more cell lines and somatic embryogenesis cycles.

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“…Start codon targeted (SCoT) marker is based on conserved regions flanking the initiation codon sequences (ATG) of genes (Satya et al 2015), by proper reproducibility (Collard and Mackill 2009) which has been employed in genetic diversity analysis of tea (Waheed et al 2017), and other crops (Luo et al 2011;Gao et al 2014;Huang et al 2014;Zeng et al 2014;Mahjbi et al 2015;Xie et al 2015;Yang et al 2015;Feng et al 2016). Both mentioned markers have also been applied to evaluate the genetic stability during micropropagation of plants (Bhattacharyya et al 2014;Rathore et al 2016;Saha et al 2016;Bhattacharyya et al 2017;Kumari et al 2017;Al-Qurainy et al 2018). However, it is the very first time that these two markers (ISSR and SCoT) are being used to assay the genetic stability or possible instability, induced by biotic or abiotic stresses and treatments such as the electromagnetic fields.…”

Section: Introductionmentioning

confidence: 99%

The assessment of electromagnetic fields-caused genetic diversity of Tea Camellia sinensis L. using ISSR and SCoT markers

et al. 2020

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The effects of electromagnetic fields on plants have been studied during the last decades. The electromagnetic fields as abiotic stress can induce genetic variation in living organisms including plants by different mechanisms. The aim of the present study is to assay the possible electromagnetic fields-caused genetic diversity of Tea Camellia sinensis L. which is known as favorite herbal beverages by many biochemical constituents. To do so, intersimple sequence repeats (ISSR) and start codon targeted (SCoT) loci were used as molecular markers. Different electromagnetic field treatments by 1, 2, 4 and 6 mT of intensity, for 30 and 60 min per day, for 7 continuous days were imposed, following by DNA extraction and PCR amplification. AMOVA, PCoA and STRU CTU RE analysis based on the ISSR and SCoT markers showed that regardless of the EMFs intensity, 30 min of radiation causes more genetic variation than that of 60 min does until the intensity was increased up to 6 mT, due to direct and indirect mechanisms such as guanine modification, methylation of cytosines, Fenton reaction and direct DNA strands breaking. ISSR sequences, however, underwent more variation than SCoT.

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Assessment of genetic stability amongst micropropagated Ansellia africana, a vulnerable medicinal orchid species of Africa using SCoT markers

Cited by 72 publications

References 55 publications

In vitro plant tissue culture: means for production of biological active compounds

In vitro plant tissue culture: means for production of biological active compounds

Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

The assessment of electromagnetic fields-caused genetic diversity of Tea Camellia sinensis L. using ISSR and SCoT markers

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