Assessment of genetic purity of four rice cultivars using microsatellite and ISSR markers

Tiwari, Jagesh Kumar; Rastogi, N. K.; Chandrakar, PK; Xalxo, Mary Suchita; Saxena, R. C.; Verulkar, S. B.

doi:10.15258/sst.2014.42.2.11

Cited by 3 publications

(5 citation statements)

References 7 publications

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“…An enzyme polyphenol oxidase (PPO) is responsible for the oxidation of externally supplied phenol into quinones and their further polymerization yield melanin like pigments catalyzed primarily by the enzyme tyrosinase in the seed coat and have resulted in development of brown coloration in seeds. The results are in conformity with findings of Singh et al [18] and Tiwari et al [2].…”

Section: Phenol Testsupporting

confidence: 93%

“…But in course of time only few varieties got stabilized and occupied major rice growing areas of the country" [1]. "Characterization of cultivars, establishment of varietal identity and genetic purity of the seed lots are crucial for varietal improvement, varietal protection and seed production" [2]. "Due to narrow genetic base and reduced range of variation among varieties chosen by the farming community coupled with the consumers' interest towards specific grain types (medium and long slender) of rice varieties, it is often becoming difficult to identify the varieties based on morphological characteristics, especially for those involved in seed certification and quality maintenance.…”

Section: Introductionmentioning

confidence: 99%

“…"Hence, in order to establish and maintain a high market reputation, all major seed companies, private and public sectors, take necessary care to ensure highest quality standards. Verification of the seed purity is essential before marketing of commercial seed" [2].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Chemical Tests in Different Indica Rice Accessions and Power of Discriminating Rice Subvarieties in North Western Himalayas

Kumari¹,

Maharana²,

Aditya³

et al. 2023

IJECC

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Variety identification has great significance from seed production, breeding as well as intellectual property rights point of view to ensure quality seed. The decrease in the morphological variation among the major rice varieties due to narrow genetic base makes it necessary for the development of quick and reliable tests for varietal identification particularly for those working in seed certification and quality maintenance. The study was conducted during 2019-20 at the laboratories of the Crop Improvement Division, ICAR-VPKAS, Almora. Two hundred Indica rice varieties/genotypes were identified on the basis of seed color (phenol, modified phenol, NaOH and KOH) to chemical tests. Phenol test grouped variety into three distinct groups viz., dark brown (15 varieties), brown (59 varieties) and light brown (95 varieties). With the help of modified phenol test these varieties were further sub grouped. NaOH test grouped varieties into two distinct groups i.e., light yellow (177 varieties) and wine red (23 varieties). These tests clearly differentiated the varieties of one group to that of another groups on the basis of seed coat color. However, KOH test was not reliable for the differentiation of these varieties because all these varieties showed light yellow color after treating with KOH solution. Though no individual chemical test was able to distinguish all the varieties, different chemical tests in conjunction were useful in identification of varieties. Thus these simple, reliable and quick tests can be used for varietal identification in rice crop.

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Section: Phenol Testsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Chemical Tests in Different Indica Rice Accessions and Power of Discriminating Rice Subvarieties in North Western Himalayas

Kumari¹,

Maharana²,

Aditya³

et al. 2023

IJECC

View full text Add to dashboard Cite

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“…Varietal verification based on morphological traits may not be appropriate and reliable because some varieties may be similar and difficult to distinguish. Ultrathin layer isoelectric focusing (UTLIEF) of seed storage proteins is a laboratory method that has great discriminative power, needs a shorter testing time, and involves lower cost than other electrophoretic methods 7,17,18 . UTLIEF has been used successfully to test the hybrid purity of tomato, maize, and sunflower and to identify tomato and rice varieties 6,7,11,17 .…”

Section: Discussionmentioning

confidence: 99%

“…UT-LIEF was also used to identify four commercial F-1 hybrid cucumbers by using water as an extraction solvent and had proposed to use a pH range 6-9 of protein band as a marker 14 . In other methods, Inter Simple Sequence Repeat (ISSR) markers could clearly identify four varieties of rice from each other with 100% identification efficacy 18 . In comparison to the UTLIEF method of the present study, the efficacy of identification was nearly the same (98%) but UTLIEF is less complicated and cheaper than the ISSR technique.…”

Section: Discussionmentioning

confidence: 99%

Varietal verification of Thai rice using ultrathin layer isoelectric focusing of seed proteins

Jiamtae¹,

Onwimol²,

Chanprasert³

et al. 2017

ScienceAsia

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ABSTRACT:A suitable extraction solvent of seed protein for the identification of Thai rice varieties was determined for use in ultrathin layer isoelectric focusing (UTLIEF). Breeder seeds of 16 varieties were used and 3 extraction buffers, namely, phosphate buffer, NaCl, and water (with gel pH 2-9) were compared. The most suitable extraction buffer was phosphate buffer, which gave the highest efficacy of identification (98%) compared to NaCl (90%) and water (89%). The phosphate buffer was then tested in varietal verification using examination of individual seeds and testing bulk samples. The results show that the method of examination of individual seeds could clearly differentiate the contaminant variety from the tested variety, while testing bulk samples (10 seeds) could identify the contaminant variety when the ratio of seed number of the contaminant variety to the tested variety was 4-6 and higher. It can be concluded that phosphate buffer is suitable to be used in UTLIEF which is a promising method for varietal verification of Thai rice.

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Genetic Divergence of Flax Genotypes (Linum Usitatissimum L.) Utilizing Microsatellite Markers

Nag

Mitra

2017

Curr Agri Res Jour

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Flax (Linum usitatissimum L.), stoods in position third, being the largest natural fibre crop and simultaneously one of the five preeminent oilseed crops in the world. SSR/microsatellite markers are extensively utilized for genetic diversity analysis and cultivar identification considering their myriad abundance, co-dominant inheritance, steep polymorphism, reproducibility, and comfort of assay by PCR. Ten microsatellites were amplified in 27 genotypes of Flax. The study was undertaken to assess the genetic diversity in flax and to select most diverse genotypes for future breeding program. Primer efficiency parameters were studied. The 10 SSR loci amplified a total of 41 alleles that were used for genetic analysis. Most primers have PIC value greater than 0.5 and the LU6 marker was highly polymorphic PIC = 0.95. Estimates of RP̅ were highest for the primer LU1 (0.68). The maximum MI was observed for the primer LU10 (3.56). The H and D ranged from 0.26 to 1.78 and 0.36 to 5.40, respectively. According to Spearman rank correlation, PIC and MI were most important parameters in assessing the efficiency of whole set of 10 SSR primers. Dendrogram was constructed using the genetic similarity coefficients using UPGMA. PCo-A was also performed in support. Genetic diversity in Flax was revealed at molecular level.

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Assessment of genetic purity of four rice cultivars using microsatellite and ISSR markers

Cited by 3 publications

References 7 publications

Characterization of Chemical Tests in Different Indica Rice Accessions and Power of Discriminating Rice Subvarieties in North Western Himalayas

Characterization of Chemical Tests in Different Indica Rice Accessions and Power of Discriminating Rice Subvarieties in North Western Himalayas

Varietal verification of Thai rice using ultrathin layer isoelectric focusing of seed proteins

Genetic Divergence of Flax Genotypes (Linum Usitatissimum L.) Utilizing Microsatellite Markers

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