Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay

Joshi, Pawan Kumar; Dhawan, Vibha

doi:10.1007/s10535-007-0005-0

Cited by 200 publications

(118 citation statements)

References 33 publications

(31 reference statements)

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“…The above observations were in conformity with previous studies on Swertia chirayita [21] and on Gerbera [22].…”

Section: Evaluation Of Scot and Ssr Markerssupporting

confidence: 93%

Efficiency of Triple-SCoT Primer in Characterization of Genetic Diversity and Genotype-Specific Markers against SSR Fingerprint in Some Egyptian Barley Genotypes

Aboulila¹,

Mansour²

2017

AJMB

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Ten Egyptian barley genotypes (2 commercial varieties and 8 breeding lines) were cultivated under normal condition at the Experimental Farm of Sakha Agricultural Research station and exposed to salinity stress condition at the Experimental Farm of El-hosainia plain Agricultural Research station, Elsharkia Governorate, Egypt, in an attempt to identify the relative salinity tolerant genotypes. A susceptibility index (SI) was used to estimate the relative stress loss because it accounted for variation in yield potential and stress intensity. Giza 123, Line-1, Line-5, Line-6 and Line-8 genotypes were considered as saline tolerant genotypes on the basis of their highly tolerance indices values. Barley genotypes were characterized by seven SSR markers and three SCoT primers in different combinations to discern the extent of genetic variation and develop a fingerprinting key. Normal SCoT reactions amplify single segments of DNA which are 15-to 19-mer long. A new strategy was used to increase SCoT potential in genetic diversity studies by using two and three different primer combinations per reaction. Amplification products scored a polymorphism percentage of 94.44% for Triple-SCoT and 90.91% for SSR, while the average no. of polymorphic fragments/primer was 17 and 7.14 in the two marker systems, respectively. On the other side, Triple-SCoT exhibited the highest average number of positive and negative genotype-specific markers. The cluster analysis of the studied genotypes using these different marker systems revealed four dendrograms varied in their topology. The dendrogram based on Triple-SCoT data exhibited the closest relationships to those illustrated by SSR dendrogram.

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“…The above observations were in conformity with previous studies on Swertia chirayita [21] and on Gerbera [22].…”

Section: Evaluation Of Scot and Ssr Markerssupporting

confidence: 93%

Efficiency of Triple-SCoT Primer in Characterization of Genetic Diversity and Genotype-Specific Markers against SSR Fingerprint in Some Egyptian Barley Genotypes

Aboulila¹,

Mansour²

2017

AJMB

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“…No polymorphism was detected during the RAPD and ISSR analysis of in vitro-raised clones. The absence of genetic variation using RAPD and ISSR marker has been reported in micropropagated shoots of Pinus thunbergii (Goto et al 1998) and swertia (Joshi and Dhawan 2007), in vitro-regenerated turmeric (Salvi et al 2001), and in vitro-raised bulblets of Lilium (Varshney et al 2001). …”

Section: Discussionmentioning

confidence: 99%

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Soni

Kaur

2013

Physiol Mol Biol Plants

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A protocol for in vitro propagation was developed for Viola pilosa, a plant of immense medicinal value. To start with in vitro propagation, the sterilized explants (buds) were cultured on MS basal medium supplemented with various concentrations of growth regulators. One of the medium compositions MS basal+0.5 mg/l BA+0.5 mg/l TDZ+0.5 mg/l GA 3 gave best results for in vitro shoot bud establishment. Although the problem of shoot vitrification occurred on this medium but this was overcome by transferring the vitrified shoots on MS medium supplemented with 1 mg/l BA and 0.25 mg/l Kn. The same medium was found to be the best medium for further in vitro shoot multiplication. 100 % root induction from in vitro grown shoots was obtained on half strength MS medium supplemented with 1 mg/l IBA. In vitro formed plantlets were hardened and transferred to soil with 83 % survival. Additionally, conservation of in vitro multiplying shoots was also attempted using two different approaches namely slowing down the growth at low temperature and cryopreservation following vitrification. At low temperature retrieval rate was better at 10°C than at 4°C after conservation of in vitro multiplying shoots. In cryopreservation-vitrification studies, the vitrified shoot buds gave maximum retrieval of 41.66 % when they were precooled at 4°C, while only 16.66 % vitrified shoots were retrieved from those precooled at 10°C. Genetic stability of the in vitro grown plants was analysed by RAPD and ISSR markers which indicated no somaclonal variation among in vitro grown plants demonstrating the feasibility of using the protocol without any adverse genetical effects.

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“…It was observed that presence or absence of variations during tissue culture depends upon the source of explant and the mode of regeneration (Goto et al 1998), including levels of growth substances that are used (Martin et al 2006). Plants regenerated from axillary buds or from other meristematic tissue showed the lowest tendency for genetic variation (Joshi and Dhawan 2007). Application of axillary branches and somatic embryogenesis are better methods for generation of genetically stable clones.…”

Section: Discussionmentioning

confidence: 99%

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

Tiwari

Chandel

Gupta

et al. 2013

Physiol Mol Biol Plants

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The genetic stability of in vitro propagated potato microtubers was assessed using random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Microtubers were developed through in vitro from potato microplants using standardized protocols. The microtubers were conserved for 1 year under three different culture media and consequently microplants were regenerated for the DNA analyses. During the study, a total of 38 (10 RAPD, 11 ISSR, 12 SSR and 5 AFLP) primers produced a total of 407 (58 RAPD, 56 ISSR, 96 SSR and 197 AFLP) clear, distinct and reproducible amplicons. Cluster analysis revealed 100 % genetic similarity among the mother plant and its derivatives within the clusters by SSR, ISSR and RAPD analyses, whereas AFLP analysis revealed from 85 to 100 % genetic similarity. Dendrogram analysis based on the Jaccard's coefficient classified the genotypes into five clusters (I-V), each cluster consisting of mother plant and its derivatives. Principal component analysis (PCA) also plotted mother plant and its genotypes of each cluster together. Based on our results, it is concluded that AFLP is the best method followed by SSR, ISSR and RAPD to detect genetic stability of in vitro conserved potato microtubers. The in vitro conservation medium (T2) is a safe method for conservation of potato microtubers to produce true-to-type plans.

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Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay

Cited by 200 publications

References 33 publications

Efficiency of Triple-SCoT Primer in Characterization of Genetic Diversity and Genotype-Specific Markers against SSR Fingerprint in Some Egyptian Barley Genotypes

Efficiency of Triple-SCoT Primer in Characterization of Genetic Diversity and Genotype-Specific Markers against SSR Fingerprint in Some Egyptian Barley Genotypes

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

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