Assessment of genetic diversity using RAPD analysis in a germplasm collection of sea buckthorn

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to characterize a part of a sea buckthorn gene bank collected for plant breeding purposes. Molecular markers were generated in 55 cultivars and accessions, representing five subspecies of Hippophae rhamnoides L. and intraspecific hybrids between different subspecies. Sixty-three markers were used to generate a Dice's similarity coefficient matrix of pairwise comparisons between individual RAPD profiles. Cluster (UPGMA) and principal co-ordinate analyses, based on this matrix, revealed clustering of plants into groups which generally correspond to their taxonomic classification or geographic origin. The analysis of molecular variance (AMOVA) was found useful for estimating components of genetic variation between and within taxonomic and geographic groups of accessions and cultivars. Whereas both alternatives for grouping the material (taxonomic or geographic origin) resulted in significant between-group variation, the major part of molecular variance (approximately 75%) was still attributed to variation within groups. We conclude that the RAPD analysis is useful for clarification of taxonomic and geographic origin of accessions and cultivars of sea buckthorn.

Changes in content of major phenolic compounds during leaf development of sea buckthorn (Hippophaë rhamnoides L.)

“…rhamnoides. When analyzed with DNA markers (RAPD) together with a set of 52 other cultivars and hybrids of sea buckthorn, these three occurred in the same main cluster together with other hybrids and a few representatives of the parental subspecies (Bartish et al 2001). However, while 'Otradnaja' and 'Gibrid Pertjika' were relatively close together also in a more narrowly defined subcluster, 'Ljubitelskaja' belonged to a different subcluster indicating that it is less closely related to the other two.…”

Development of RNA-Seq SSR Markers and Application to Genetic Relationship Analysis among Sea Buckthorn Germplasm

“…Molecular markers are widely used as a tool for the analysis of genetic diversity, including clarification of the taxonomic and geographic origin of cultivars, sex determination, and population genetic structure of woods. In fact, various types of molecular markers have been widely used to distinguish among sea buckthorn cultivars, e.g., amplified fragment length polymorphism, random amplified polymorphic DNA, intersimple sequence repeat, and sequence-related amplified polymorphism (Bartish et al, 2001;Li et al, 2009Li et al, , 2010Ruan, 2006). Compared with the molecular markers mentioned above, SSR (microsatellite) markers, which are 1 to 6 bp DNA regions repeated in tandem, have desirable advantages, such as codominance, random distribution throughout the genome, a high level of polymorphisms, high reproducibility, and technical simplicity (Kalia et al, 2011).…”

Synthetic, sterochemical, and electronic studies of organophosphorous ligands and their transition metal complexes

“…The product was purified prior to use by sublimation at 0.5 torr and 50°C. The and"NMR data agreed with those previously reported (75) and are given in Tables 7 and 8 Gr(C0)g (26), pentacarbonyT(2-ch1oro-5,5-dimethy1-l,3,2dioxaphosphorinano)chromium (38) This complex was prepared using a modification of the procedure of Bartish and Kraihanzel (79) obtained for the product agreed well with that previously reported (79) and both the and data are given in Tables 10 and 11, respectively.…”