Assessment of genetic diversity of pumpkins (Cucurbita spp.) from western Kenya using SSR molecular markers

Nyabera, Lameck A; Nzuki, Inosters; Runo, Steven; Amwayi, Peris

doi:10.1007/s11033-021-06245-9

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…The SSR marker GMT-P25 was the most polymorphic with a PIC value of 0.202. SSR markers with high PIC value are, therefore, potential candidate markers that can be used for genetic variation studies for squash genotypes [12]. Based on genetic structure analysis and UPGMA analysis, 3 groups were identi ed.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Genetic Diversity of Some Squash (Cucurbita Maxima L.) Germplasm Using Morphological and Molecular Markers in Erzincan

Öztürk

Dönderalp²,

Bulut

et al. 2021

Preprint

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Background Plant genetic resources constitute the most valuable assets of countries. It is of great importance to determine the genetic variation among these resources and to use the data in breeding studies. Cucurbita maxima species in the cucurbitaceae family have high genetic diversity, but its genetic diversity at the molecular level is inadequately characterized. Methods and Results To determine the genetic diversity among genotypes of Cucurbita maxima species of squash, which is widely grown in Erzincan, 14 different squash genotypes collected were examined based on the morphological parameters and molecular characteristics. SSR (Simple sequence repeat) markers were used to determine genetic diversity at the molecular level. The analysis of morphological characterization within genotypes showed a wide variability in morphological traits of plant, flower, fruit, and leaf. Seven SSR markers yielded a total of 23 polymorphic bands, the number of alleles per marker ranged from 2 to 5, and the mean number of alleles was 3.286. Polymorphic information content (PIC) ranged from 0.00 (GMT-M61) to 0.202 (GMT-P25), and the mean PIC value per marker was 0.130. Cluster analysis using Nei's genetic distance determined that 14 genotypes were divided into 3 major groups. Conclusions The SSR markers used were effective in distinguish among similar winter squash or pumpkin and therefore can be beneficial for consideration of Cucurbita maxima species diversity, screening of genetic resources and their selection.

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Section: Discussionmentioning

confidence: 99%

Exploring the Genetic Diversity of Some Squash (Cucurbita Maxima L.) Germplasm Using Morphological and Molecular Markers in Erzincan

Öztürk

Dönderalp²,

Bulut

et al. 2021

Preprint

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“…Twenty independent runs were performed for each K value ranging from 1 to 20. The most likely number of clusters was evaluated using K, and the log-likelihood value was evaluated with Structure Harvester v0.6.94 [21]. All analyses were based on the SSR data.…”

Section: Genetic Diversity Analysismentioning

confidence: 99%

“…12, x FOR PEER REVIEW 9 of 16 second principal components are shown in the PCoA chart (Figure3). According to the principal components based on the K-means[21], the left and right sides of the first principal component were clustered into one category, and the upper and lower right sides were clustered into two categories. A close chart indicated high genetic similarity.…”

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confidence: 99%

Genetic Diversity Analysis and Potential Distribution Prediction of Sophora moorcroftiana Endemic to Qinghai–Tibet Plateau, China

Yang

et al. 2021

Forests

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Sophora moorcroftiana (Benth.) Baker is an endemic woody species distributed in the Qinghai–Tibet Plateau (QTP), a part of the world characterized by high altitude and cold weather. In this study, the genetic diversity of S. moorcroftiana was evaluated based on 300 representative samples of 15 populations using 20 polymorphic SSR markers, and its potential distribution was predicted according to 19 bioclimatic factors using MaxEnt modeling. Results showed the population genetic diversity of S. moorcroftiana was generally not high (around 0.5), and the range of variation was small (0.437–0.539). Altitude, rather than other environmental factors, was the key factor affecting the present genetic diversity. Moreover, due to climate change in the QTP, the suitable area is increasing and will continue to increase by 48.35%, 84.44%, 101.98%, and 107.30% in the four future periods of 2030s, 2050s, 2070s, and 2090s, respectively, compared to the present, which is beneficial for S. moorcroftiana. These results will provide a theoretical basis for the development of germplasm conservation strategies for S. moorcroftiana and enrich information on the impacts of climate change on plants in the QTP.

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“…are one of the most overlooked and underutilized food and medicinal plants. Its production is hindered by a scarcity of genetically improved seeds [9]. It is an extraordinary vegetable with the potential to be used as a medicinal as well as a nutritious multifunctional food.…”

Section: Introductionmentioning

confidence: 99%

Pumpkin (Cucurbita spp.): A Crop to Mitigate Food and Nutritional Challenges

et al. 2021

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The world’s food and agricultural programs have gradually declined into an unsustainable situation due to challenges such as increase in world population, varied agro-climatic regions, increase temperature, extreme sole-culture growing techniques, and water shortage. A considerable emphasis has been put on few staple food crops coupled with repeated dieting, food scarcity, and essential mineral deficits, frequently inducing dietary disorders. Because relying on staple crops may lead to serious food shortages in the future, we must adjust our dietary habits to include a diverse range of non-staple foods and maximize their use in order to achieve food security and reduce the nutritional gap. To assure healthy meals around the world, an authentic and reasonable strategy is presented to draw additional awareness towards variations in agricultural production techniques and dietary preferences. The EAT-Lancet declaration highlighted the importance of increasing agri-based foods to achieve sustainable health. Expanding overlooked crops with abundant genetic stocks and possibly beneficial characteristics is an approach that might meet food and nutritional security challenges. Although undervalued, pumpkin is a valuable vegetable herbaceous plant that contributes to global food and nutritional security. This crop has already been identified as a revolutionary age crop, balanced food, and more adapted to low soil and atmospheric circumstances than other major crops. This review paper focuses on the potential uses of pumpkin as an underutilized crop; diversification and development of hybrids, particularly hybridization breeding through diallel mating design; and how implementation of this “modern” technology would contribute to the breeding of the neglected pumpkin vegetable and stimulate productivity and nourish the world’s largest malnourished, deprived, and starved populations.

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Assessment of genetic diversity of pumpkins (Cucurbita spp.) from western Kenya using SSR molecular markers

Cited by 11 publications

References 27 publications

Exploring the Genetic Diversity of Some Squash (Cucurbita Maxima L.) Germplasm Using Morphological and Molecular Markers in Erzincan

Exploring the Genetic Diversity of Some Squash (Cucurbita Maxima L.) Germplasm Using Morphological and Molecular Markers in Erzincan

Genetic Diversity Analysis and Potential Distribution Prediction of Sophora moorcroftiana Endemic to Qinghai–Tibet Plateau, China

Pumpkin (Cucurbita spp.): A Crop to Mitigate Food and Nutritional Challenges

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