Assessment of Genetic Diversity of Some Wild Populations of Thymus Kotschyanus Usingrapd Molecular Markers

Abstract

RAPD molecular markers were used to assess the genetic diversity of some population of thyme (Thymus kotschyanus) from Iran. The 17 tennucleotide primers used that produced 185 high-resolution bands, which 21 of these were monomorphic and 162 were polymorphic. An average, 10.88 bands were obtained per primer and 9.52 of these were polymorphic. POPGENE software was used to calculate Nei's genetic distance among populations and based on the cluster analysis of this matrix, a UPGMA dendrogram was drawn using the software NTSYS-pc. On the basis of dendrogram among populations of wild mountain thyme, Avan (Qazvin) and Siahkal (Gilan province) populations was the most similar between studied populations with the distance of 0.094 high disimilarity was observed between Qazvin and Mazandaran 2 populations with the distance of 0.185 within populations variation based on Shannon's information index and Nei genetic diversity index showed that Takestan (I= 0.26; H= 0.3) and Alamut (I= 0.24; H= 0.15) had a highest and lowest within populations variation compared to other populations, respectively. Average of Fst and Nm indices, which represent the amount of gene flow between populations, were recorded as 0.26 and 1.361, respectively, which reflects the high level of gene exchange between ten populations of Thymus kotschyanus.

Molecular Characterization of Endangered Sinai Thyme (Thymus Decussatus Benth.) Growing on Gebel Mousa, South Sinai, Egypt

“…Our results were entirely compatible with results on five Palestinian Thymus species, T. kotschyanus, T. caespititius, [28,29,38]. In addition, numerous research studies on Thymus species from several countries showed that the rate of polymorphism ranges from 62% to 92% [39,40,[41][42][43].…”

“…High level of polymorphism recorded in our study reflects the great degree of genetic variation among Thymus populations collected from various microhabitats in Gebel Mousa. Such heterogeneity may be due to the high degree of gene exchange between the studied populations [29].…”

“…RAPD markers were also used in genetic identification of five accessions of Palestinian Thymus for their ability to produce polymorphisms [28].Moreover, genetic diversity of ten Thymus kotschyanus populations obtained from various ecosystems in Iran was analyzed using RAPD markers [29].Their findings showed high level of genetic diversity among the ten studied populations of T. kotschyanus. Even, twenty ISSR primers were applied for evaluation of the genetic relationships among fourteen Thymusaccessionsbelonging to three T. daenensis, T. kotschyanus and T. vulgaris species [30].…”

Evaluation of the Genetic Variability among a Wild Peganum Harmala L. Populations with RAPD-PCR

“…cell growth inhibition and in higher concentration/longer time, complete cell death occurred Tehrani et al [1] Assess the diversity of plant genetic resources (germplasm), that have a great chance to occurrence of useful genes, is the first essential prerequisite to plan for the conservation, sustainable use and domestication of them Khoshsokhan et al [2]. Molecular markers such as random amplified polymorphic DNA Williams et al [3].…”