Assessment of genetic diversity of bermudagrass germplasm from southwest China and Africa by using AFLP markers

Abstract

ABSTRACT. Cynodon dactylon (L.) Pers. var. dactylon (common bermudagrass) is widely distributed geographically between approximately 45°N and 45°S latitude, penetrating to approximately 53°N latitude in Europe. The extensive variation of morphological and adaptive characteristics of the taxon has been substantially documented, but information is lacking on DNA molecular variation in geographically disparate forms. The genetic diversity of 51 wild accessions of bermudagrass from southwest China (Sichuan, Chongqing, Yunnan, Guizhou, and Tibet) and 8 African bermudagrass was analyzed using amplified fragment length polymorphism molecular markers. A total of 670 polymorphic bands were detected with 11 primer combinations, of which 663 (98.74%) bands were found to be polymorphic. The genetic similarity among the accessions ranged from 0.64-0.96 with an average of 0.78. All 59 wild accessions were clustered into 5 ecogeographic groups, and nearly all accessions from the same area were classified into the same group and were found to be associated with their geographical distributions. Therefore, complex geographical and 1749 Genetic diversity of wild Cynodon dactylon ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 1748-1756 (2015) ecological environments are important factors for the genetic structure and geographical distribution of C. dactylon.

Genetic diversity and population structure of Chinese natural bermudagrass [Cynodon dactylon (L.) Pers.] germplasm based on SRAP markers

“…Molecular markers have significant advantages over morphological and isozyme markers because they are uninfluenced by growth and environmental conditions and can be applied from any growth phase. A wide variety of molecular marker types have been applied to evaluate the genetic diversity of bermudagrass, including DNA amplification fingerprinting (DAF) [8–10], randomly amplified polymorphic DNA (RAPD) [11,12], amplified fragment length polymorphism (AFLP) [13–17], inter-simple sequence repeat (ISSR) [12, 18–21], simple sequence repeat (SSR) [21–23], peroxidase gene polymorphism (POGP) [12] and sequence-related amplified polymorphism (SRAP) [12, 24, 25] markers.…”

“…Several studies have investigated the genetic diversity of Chinese wild bermudagrass based on DNA molecular markers [14, 15, 17–19, 21–23, 25]. The studies mentioned above investigating the genetic diversity and relationships of bermudagrass accessions were mainly based on traditional cluster analysis which could provide an easy and effective method in estimating the genetic diversity of accessions [27].…”

Genetic diversity and species‐specific DNA markers ofCynodon

“…Numerous studies of C. dactylon revealed high genetic diversity within this species. In many studies, accessions listed as C. dactylon grouped not only according to the country of origin (Huang, Huang, Zhang, & Liu, 2010; Ling et al., 2015; Wu et al., 2004) but significant amounts of diversity were also observed within any one country or region, as shown by studies in China, Iran, and Korea (Etemadi et al., 2006; Huang et al., 2014; Kang et al., 2008). Here, a similar pattern for C. dactylon var.…”

“…Genetic diversity of C. dactylon in China was extensively investigated using amplified fragment length polymorphism (AFLP), DNA amplification fingerprinting (DAF), simple sequence repeats (SSR), inter simple sequence repeat (ISSR), and sequence‐related amplified polymorphism (SRAP) markers (Huang, Liu, Bai, & Wang, 2014; Li, Liu, Lou, Hu, & Fu, 2011; Ling et al., 2012, 2015; Wang, Liao, Yuan, Guo, & Liu, 2011; Wang et al., 2013; Wu et al., 2006; Xie et al., 2015; Zheng, Xu, Liu, Zhao, & Liu, 2017). Several studies were performed to establish relatedness among C. dactylon accessions from Asia, Africa, and Australia (Ling et al., 2015; Wang et al., 2011, 2013; Zhang et al., 1999). Wu, Taliaferro, Bai, and Anderson (2004) used AFLP markers to analyze C. dactylon (L.) Pers.…”

Relevance of Allergenic Sensitization to Cynodon dactylon and Phragmites communis: Cross-reactivity With Pooideae Grasses

“…However, there are other subfamilies with species capable of inducing allergic symptoms, such as Chloridoideae (Cynodon dactylon) and Arundinoideae (Phragmites communis). Both C dactylon and P communis have been identified in warm temperate and subtropical areas in Africa, Asia, Australia, and America [1], and also in Europe up to a latitude of approximately 53°N [2]. Although C dactylon is now cosmopolitan, it is generally recognized that its present distribution is largely due to human activities [3] as it is used as livestock herbage and turf.…”