Assessment of Genetic Diversity in Contrasting  Sugarcane Varieties Using Inter-Simple Sequence Repeat (ISSR) Markers

Costa, Maria Lucília M. da; Amorim, Lidiane Lindinalva Barbosa; Onofre, Alberto Vinicius Casimiro; Melo, Luiz José Oliveira Tavares de; Oliveira, Maria Betânia Melo de; Carvalho, Reginaldo de; Benko‐Iseppon, Ana Maria

doi:10.4236/ajps.2011.23048

Cited by 28 publications

(22 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…hybrid) is a crop of industrial importance in the tropical and sub-tropical regions of more than 100 countries around the globe (daCosta et al 2011). In the year 2014, with a total sugarcane production of 352.1 mT from 5.01 mha, India ranked second only to Brazil in cane area (10.4 mha) and sugarcane production (737.1 mT; FAO 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of marker-trait associations for morphological descriptors and yield component traits in sugarcane

Siraree

Banerjee

Khan

et al. 2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Ninety two sugarcane varieties from sub-tropical India were subjected to molecular profiling with 174 simple sequence repeat markers and characterized for 23 qualitative (morphological descriptors) and nine quantitative traits that directly or indirectly contribute to yield and juice quality. Using STRUCTURE-based population stratification study and a mixed linear model for marker-trait association (MTA) analysis, a total of 60 MTAs were identified for 22 qualitative traits that were able to explain a significantly higher (up to 40%) proportion of the phenotypic variations compared to all the previous reports of MTA studies in sugarcane. In addition, 21 MTAs stable over the three years of study were also identified for nine quantitative traits that explained 16-37% of the total trait variation. It could be concluded that the qualitative traits that are governed mostly by one or a few genes are more responsive to MTA studies and hence have a better potential to be adopted in marker-assisted breeding programmes in sugarcane. The MTAs identified in this study could also find significant applications in upcoming more stringent IP regime, which may necessitate tracking of specific alleles integrated in breeding programmes.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of marker-trait associations for morphological descriptors and yield component traits in sugarcane

Siraree

Banerjee

Khan

et al. 2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…Twenty-one primers (Table 2) previously screened in Miscanthus species and Saccharum officinarum (sugarcane) via ISSR PCR analysis (Hodkinson et al 2002;da Costa et al 2011) were used to detect polymorphisms. Amplifications were performed using 100 ng template DNA, 100 μM of each dNTP, and 2 mM MgCl 2 in a 50-μl reaction mix using 0.5 units of Taq polymerase as described by Perera et al (2015).…”

Section: Methodsmentioning

confidence: 99%

Direct and indirect in vitro regeneration of Miscanthus × giganteus cultivar Freedom: effects of explant type and medium on regeneration efficiency

Perera

Barnes

Baldwin

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Miscanthus × giganteus (giant miscanthus; Mxg) is a seed-sterile, perennial bioenergy crop with the potential to produce liquid fuel from lignocellulosic biomass. A new cultivar, Freedom, is being commercially grown in the USA on increasing acreage. To determine this genotype's regeneration responses in tissue culture, three explant sources were screened on media proven successful for other genotypes. Four callus induction media contained 13.6-22.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) alone or with 0.44-4.4 μM 6-benzyladenine (BA). Callus induction percentages for all explants ranged from 93 to 97%. Media yielding the greatest percentages of explants producing regenerable calli for shoot apices (from in vitro and greenhouse plant sources) were media containing either 13.6 μM 2,4-D plus 0.44 μM BA or 22.6 μM 2,4-D plus 0.44 μM BA. After culture on a regeneration medium containing 22 μM BA plus 1.3 μM naphthaleneacetic acid (NAA), 3.59-3.74 regenerants were obtained per explant. Immature inflorescence explants (from field-maintained plants) gave up to 77% regenerable calli and 6.99 regenerants per explant. Direct regenerants (shoots) arose from immature inflorescence explants on a medium containing 9.0 μM 2,4-D. Intact plants could be generated within 16-18 wk after culture initiation. Extensive visual assessments, and molecular assessments via inter-simple sequence repeat (ISSR) PCR analysis using 21 different primers, did not reveal distinguishable somaclonal variation among regenerants or when compared to rhizome-propagated transplants under field conditions. We believe that this is the first extensive in vitro and ex vitro analysis on a commercially grown Mxg genotype.

show abstract

“…Drought decreased sugarcane productivity through some morpho-physiological effects (Andrade et al 2015), it reduced stalk height, leaf area and stalk diameter (Cia, M. C et al 2012 andJangpromma et al 2012). Molecular markers were used as a marker-assisted selection (MAS) of sugarcane (Costa et al,2011, Khaled et al 2011and 2016. Khaled et al 2011 used RAPD, ISSR and R-ISSR techniques for detecting markers associated to sugar content.…”

Section: Introductionmentioning

confidence: 99%

R-ISSR As Marker Assisted Selection For Drought Tolerance In Sugarcane

Khaled

sherbeny

Abdelhadi

2017

Journal of Agricultural Chemistry and Biotechnology

View full text Add to dashboard Cite

Sugarcane is one of the most important crops in the world for its syrup and by-products. Eight sugarcane genotypes (i.e.G.T.54-9, F.153, Co.997, Co.775, and F.161) were kindly provided by Sugar Crops Research Institute (SCRI), they were selected depending on previous screening of SCRI germplasm for studying the efficiency of technique that combined Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSRs) as marker to assist selection for drought tolerance in sugarcane and compared it with RAPD and ISSR each separately. This technique is called R-ISSR.The performances of studied genotypes revealed that RAPD, ISSR, and R-ISSR techniques are useful as marker assisted selection for drought tolerance in sugarcane. 26 positive and negative markers were obtained from used techniques (RAPD, ISSR and R-ISSR), which would be used as marker to assist selection for drought tolerance. The study revealed that R-ISSR technique was more efficiency in this case. The study encourages breeder to use R-ISSR technique in the early selection for drought tolerance through sugarcane breeding programs.

show abstract

Assessment of Genetic Diversity in Contrasting Sugarcane Varieties Using Inter-Simple Sequence Repeat (ISSR) Markers

Abstract: ABSTRACT

Cited by 28 publications

References 29 publications

Identification of marker-trait associations for morphological descriptors and yield component traits in sugarcane

Identification of marker-trait associations for morphological descriptors and yield component traits in sugarcane

Direct and indirect in vitro regeneration of Miscanthus × giganteus cultivar Freedom: effects of explant type and medium on regeneration efficiency

R-ISSR As Marker Assisted Selection For Drought Tolerance In Sugarcane

Contact Info

Product

Resources

About