Assessment of genetic diversity in indigenous turmeric (Curcuma longa) germplasm from India using molecular markers

Verma, Sushma; Singh, Shweta; Sharma, S. C.; Tewari, Sanchita; Roy, R. K.; Goel, Anil Kumar; Rana, T. S.

doi:10.1007/s12298-015-0286-2

Cited by 28 publications

(18 citation statements)

References 33 publications

(25 reference statements)

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“…A dichotomous key and a dendrogram for the species were also developed. Sushma et al [24] carried out an assessment of genetic diversity in indigenous turmeric (Curcuma longa) germplasm from India using directed amplification of minisatellite DNA (DAMD) and inter-simple sequence repeats (ISSR) markers and reported 82 % polymorphism across the turmeric genotypes. Paul and his co-researcher [25] reported a wide genetic variation among the different turmeric accessions using RAPD analysis.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity in Accessions of Indian Turmeric (Curcuma Longa L.) Using Rapd Markers

Ashraf

Ahmad

Shah

et al. 2017

Int J Pharm Pharm Sci

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Objective: The present investigation was undertaken for identification and assessment of eight accessions of Curcuma longa collected from all ecological zones in India by random amplification of polymorphic DNA (RAPD) markers.Methods: DNA was isolated using modified cetyl trimethyl ammonium bromide (CTAB) method. Polymerase chain reaction (PCR) was performed according to the method based on Williams et al. and data analysis was done using Alpha Imager EC software.Results: Eleven out of twenty primers screened were informative and produced 150 amplification products among which 132 products (88%) were found to be polymorphic. The percentage polymorphism of all 08 accessions ranged from 44.44% to 100%. A total of 150 amplification products were scored with an average frequency of 13.63 bands per primer. Most of the RAPD markers studied showed a different level of genetic polymorphism. The data of 150 RAPD bands were used to generate Jaccard's similarity coefficients and to construct a dendrogram by means of UPGMA. Conclusion:Results shows that C. longa undergoes genetic variation due to a wide range of ecological conditions within distribution area of its population in India. This investigation as an understanding of the level and partitioning of genetic variation within the accessions and would provide an important input into determining efficient management strategies and will help to breeders for turmeric improvement program.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity in Accessions of Indian Turmeric (Curcuma Longa L.) Using Rapd Markers

Ashraf

Ahmad

Shah

et al. 2017

Int J Pharm Pharm Sci

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“…Kunyit diperbanyak secara vegetatif menggunakan rimpang karena jarang menghasilkan bunga dan biji serta memiliki sterilitas yang tinggi karena memiliki kromosom triploid (2n=63) (Ravindran et al 2007). Di sisi lain, penelitian menunjukkan adanya perbedaan genetik antara genotip kunyit di India pada tingkat gen meskipun tanaman tersebut diperbanyak secara vegetatif (Verma et al 2015;Singh et al 2015;Singh et al 2018). Keragaman genetik pada tanaman yang diperbanyak vegetatif dapat terjadi melalui beberapa mekanisme seperti mutasi alami dan warisan epigenetik transgenerasi (Balloux et al 2003).…”

Section: Pendahuluanunclassified

“…Menurut Singh et al (2018) rentang klaster aksesi kunyit yang berada pada rentang 0,44 -1,00 mengindikasikan adanya keragaman. Sementara pada penelitian Verma et al (2015), jarak genetik 30 genotip kunyit yang berada pada rentang 0,03-0,59 menunjukkan cukup beragam. Pada penelitian ini, jarak genetik pada klaster sangat bervariasi terutama jarak koefisien kemiripan di atas 0,45 yang menunjukkan keragaman yang luas.…”

Section: Analisis Kekerabatan Dan Keragaman Genetikunclassified

Keragaman Genetik 64 Aksesi Kunyit Asal Indonesia Berdasarkan Marka P450-Based Analogue (Pba)

et al. 2020

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<em>Turmeric is a rhizome producing plant with many utilization such as for consumption, medicine, and colorant industries. The development of superior turmeric varieties in Indonesia needs to be supported by genetic diversity information availability. Despite its potential, genetic diversity information of Indonesian turmeric has not been widely observed. A molecular marker is used to address genetic diversity information with the accurate result due to minimum environmental influences. PBA can detect the P450 gene as a functional marker, which is related to the synthesis of secondary metabolites in a wide genome area. Thus, it can be used as an alternative marker to identify genetic diversity. This research aimed to obtain genetic diversity information of 64 turmeric accessions using eight primer sets of P450-Based Analogue (PBA). The study was conducted in the Central Laboratory of Padjadjaran University from June 2019 to January 2020. Results showed that the full 133 bands were detected with a range of allele number 8 - 45 bands and an average of 22.3 bands per allele. PIC analysis showed six primer sets of PBA had high polymorphisms ranged from 0.90 to 0.98, hence categorized </em><em>PBA as a highly informative marker. Cluster analysis divided 64 turmeric accessions into two main clusters based on a similarity index ranged from 0.01 to 0.83. The accession of CL-GTL01 origins from Gorontalo had a low similarity coefficient of 0.01 to the other 64 accessions cluster. On the other hand, CL-NTB01 dan CL-PPB01 had the highest similarity index of 0.83. Based on the PIC value, the total number of polymorphic bands, and genetic distance, it can be concluded that local Indonesian turmeric had wide diversity based on PBA marker.</em>

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“…RAPD-PCR mixture was initially denatured at 94°C for 6 min, followed by 40 cycles of 94°C for 1 min, 36-38°C (depending on primer composition) for 1 min, 72°C for 1 min, and final extension at 72°C for 8 min. ISSR primers used in the present study were designated from the previous reports of Mohanty et al (2014) and Verma et al (2015). ISSR-PCR mixture was subjected to initial denaturation at 94°C for 10 min followed by 35 cycles of denaturation at 94°C for 1.3 min, annealing at 40-68°C (depending on primer composition) for 1 min and extension at 72°C for 2 min with a final extension at 72°C for 10 min.…”

Section: Rapd and Issr Amplificationmentioning

confidence: 99%

Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers

et al. 2017

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Costus pictus, belonging to the family Costaceae, is one of the valuable medicinal plants with its antidiabetic property. Despite ever-increasing demand from the pharmaceutical industry, this species is being less exploited at molecular level. Hence, an effort has been made in the present study to characterize the 15 accessions of C. pictus collected from different geographical regions of India through random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. A total of 25 RAPD and 20 ISSR primers were used in the present study. The RAPD analysis generated 343 loci, of which 124 were polymorphic with an average of 4.96 loci per primer. While, ISSR primers produced 177 loci, of which 77 were polymorphic with an average of 3.85 loci per primer. The similarity coefficients ranged from 0.86-0.99, 0.84-0.95 and 0.86-0.96 for RAPD, ISSR and combined RAPD-ISSR, respectively. The UPGMA dendrogram generated using these data showed low level of divergence among the accessions from South and West regions. Further, accession-specific bands were also revealed by RAPD and ISSR markers which might be contributed to specific trait. This investigation was an understanding of genetic variation within the C. pictus accessions. The present finding indicates that both the marker tools RAPD and ISSR combined or individually can be used in determining the genetic relationship between the accessions. It may be concluded that data of hereditary differences appeared among the C. pictus accessions could be utilized for their conservation and reproducing programs.

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Assessment of genetic diversity in indigenous turmeric (Curcuma longa) germplasm from India using molecular markers

Cited by 28 publications

References 33 publications

Genetic Diversity in Accessions of Indian Turmeric (Curcuma Longa L.) Using Rapd Markers

Genetic Diversity in Accessions of Indian Turmeric (Curcuma Longa L.) Using Rapd Markers

Keragaman Genetik 64 Aksesi Kunyit Asal Indonesia Berdasarkan Marka P450-Based Analogue (Pba)

Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers

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