Assessment of genetic diversity and population structure of Eulaema nigrita (Hymenoptera: Apidae: Euglossini) as a factor of habitat type in Brazilian Atlantic forest fragments

Abstract

In many organisms, habitat affects ecological and genetic diversity and, for certain species, it is expected that a large population should have higher genetic diversity than a smaller population. We analysed the genetics of males of the orchid bee, Eulaema nigrita (Hymenoptera: Apidae) and examined the links between local abundance and habitat with genetic diversity. We also investigated the impact of landscape features on genetic population structure, through microsatellite and mitochondrial DNA analysis, among populations found across 700 km of Brazilian Atlantic forest fragments. We found that genetic variation was not a function of local abundance, but it correlated significantly with habitat. There was significant but low genetic differentiation among populations. We found a single mitochondrial DNA haplotype in all sequenced specimens, which may be widespread throughout the Atlantic forest. Our results reinforce the idea that orchid bees can fly long distances. High-dispersal capacity, together with insufficient time in eventual isolation, can directly contribute to the high degree of similarity among populations for this species, despite their wide geographical distribution.

No disruption of rhizobial symbiosis during early stages of cowpea domestication

“…2016; Costa et al. 2021). The GLMM with a Beta distribution and a logit link function was modeled using the package glmmTMB (Brooks et al.…”

“…One to 10 ancestral populations (i.e., entropy criterion; K = 1-10) were assumed using 100 repetitions. To test if patterns of genetic diversity differed among populations, a generalized mixed model analysis using SNP loci as our random factor was implemented (Kamvar et al 2016;Costa et al 2021). The GLMM with a Beta distribution and a logit link function was modeled using the package glmmTMB (Brooks et al 2017;Douma and Weedon 2019).…”