Assessment of genetic diversity among and within Carthamus species using sequence-related amplified polymorphism (SRAP) markers

Mokhtari, Niloofar; Rahimmalek, Mehdi; Talebi, Majid; Khorrami, Mojtaba

doi:10.1007/s00606-013-0796-8

Cited by 29 publications

(24 citation statements)

References 31 publications

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“…Safflower, Carthamus tinctorius L., an Asteraceae, is cultivated in semi-arid regions mainly for its seed that contains a high level of oil. Safflower oil contains saturated and unsaturated fatty acids coupled by its tocopherol content [1]. Other compounds are also present in safflower oil.…”

Section: Introductionmentioning

confidence: 99%

Phenol Content and Antioxidant and Antiaging Activity of Safflower Seed Oil (Carthamus Tinctorius L.)

et al. 2019

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The phenol content of vegetable oil and its antioxidant activity are of primary interest for human health. Oilseed species are considered important sources of these compounds with medicinal effects on a large scale. Total phenol content (TPC) and antioxidant activity (AA) of safflower oil were previously studied. Nevertheless, there is no report on genotypic differences and antiaging activity of safflower oil. The aim of this study was to determine the TPC, diphenyl-picrylhydrazyl (DPPH), and antiaging activity on three respective accessions from Syria, France, and Algeria of seed oil of safflower grown under semi-arid conditions during 3 consecutive years (2015, 2016, and 2017). The results showed that phenol content as well as antioxidant and antiaging activity varied according to both genotype and years. In 2017, the mean value of TPC in oil seed was two times higher than in 2015 and 2016. Moreover, accessions presented different TPC values depending on the year. The highest antioxidant activity was observed among accessions in 2017 compared to 2015 and 2016. As expected, a positive correlation was found between TPC and antioxidant activity. The inhibition in the collagenase assay was between 47% and 72.1% compared to the positive control (83.1%), while inhibition in the elastase assay of TPC ranged from 32.2% to 70.3%, with the positive control being 75.8%. These results highlight the interest of safflower oil as a source of phenols with valuable antioxidant and antiaging activity, and uses for cosmetics.

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Section: Introductionmentioning

confidence: 99%

Phenol Content and Antioxidant and Antiaging Activity of Safflower Seed Oil (Carthamus Tinctorius L.)

et al. 2019

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“…The protocol is simple, efficient, and has a high production rate. It has been used successfully in genetic diversity analysis and construction of genetic maps of many plant species [14][15][16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Toona Ciliata Roem. Based on Sequence-Related Amplified Polymorphism (SRAP) Markers

Zhan

Que

et al. 2015

Forests

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Sequence-related amplified polymorphism (SRAP) markers were used to investigate the genetic diversity among 30 populations of Toona ciliata Roem. sampled from the species' distribution area in China. To analyze the polymorphism in the SRAP profiles, 1505 primer pairs were screened and 24 selected. A total of 656 SRAP bands ranging from 100 to 1500 bp were acquired, of these 505 bands (77%) were polymorphic. The polymorphism information content (PIC) values ranged from 0.32 to 0.45, with an average of 0.41. An analysis of molecular variance (AMOVA) indicated that the most significant variation was attributable to differences among the populations and that variation within the populations was OPEN ACCESSForests 2015, 6 1095 small. STRUCTURE analysis divided the 30 populations into two parts. The unweighted pair group method of arithmetic averages (UPGMA) clustering and principal coordinates analysis (PCoA) showed that the 30 populations could be classified into four types. The results demonstrate a clear geographical trend for T. ciliata in China and provide a theoretical basis for future breeding and conservation strategy of T. ciliata.

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“…Application of morphological descriptors and geographical origin to assess the variability of around 2000 lines of safflower revealed a wide range of phenotypic variation for several traits, based on which safflower accessions were classified into 10 "regional gene pools" (Ashri 1975). Several recent studies used molecular markers, viz., random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), sequence-related amplified polymorphism (SRAP), and simple sequence repeat (SSR), for variation analysis either exclusively or in conjunction with morphological data for the crop (Sehgal and Raina 2005;Johnson et al 2007;Yang et al 2007;Amini et al 2008;Peng et al 2008;Chapman et al 2009Chapman et al , 2010Khan et al 2009;Mahasi et al 2009;Hamdan et al 2011; Barati and Arzani 2012;Mokhtari et al 2013;Lee et al 2014). However, low sample size from selected regional gene pools and lack of a global representation of available germplasm were limitations in most of these studies, which may therefore not provide an accurate representation of the available genetic diversity and population structure in safflower.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity and Population Structure in a Global Reference Collection of 531 Accessions of Carthamus tinctorius L. (Safflower) Using AFLP Markers

et al. 2014

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Carthamus tinctorius L. (safflower) is an important oilseed crop that is cultivated in several countries. The present study investigates the genetic diversity and population structure of 531 safflower accessions from 43 countries representing all safflower growing regions of the world. Diversity analysis was performed using ten informative EcoRI/ MseI amplified fragment length polymorphism primer pairs that were identified by screening 150 primer combinations. The selected primer pairs generated 381 fragments of which 157 were polymorphic among the analyzed accessions. The genetic diversity indices obtained for the entire collection (I= 0.4536, H=0.2955) indicated high levels of molecular variability. The distance-based, neighbor-joining method classified the accessions into six clusters with internal subgroupings that were in consonance with 19 clusters obtained using Bayesian model-based BAPS analysis. Clusters obtained through STRUCTURE analysis (at K=4) could not be correlated with their geographically diverse origins, while BAPS analysis (at K=19) revealed geographical delineation with low admixture levels among most of the studied accessions. Accessions from Far East and Egypt clustered in distinct groups, indicating conserved nature of their gene pools. The Near East and Iran-Afghanistan regions were collectively found to harbor maximum diversity in accordance with earlier reports. Accessions from the Indian subcontinent showed substantial diversity that was previously undetected. The American accessions showed low molecular variability in contrast to earlier studies. Genetic sub-structuring within gene pools and interrelationships between accessions belonging to different regional pools was also observed. To the best of our knowledge, this is the first comprehensive study of existing genetic variability in a large collection of safflower germplasm with a global distribution, which provides a more accurate representation of genetic structuring in the crop. This information will facilitate selection of elite genotypes for broadening the genetic base of various breeding programs in safflower.

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Assessment of genetic diversity among and within Carthamus species using sequence-related amplified polymorphism (SRAP) markers

Cited by 29 publications

References 31 publications

Phenol Content and Antioxidant and Antiaging Activity of Safflower Seed Oil (Carthamus Tinctorius L.)

Phenol Content and Antioxidant and Antiaging Activity of Safflower Seed Oil (Carthamus Tinctorius L.)

Genetic Diversity and Population Structure of Toona Ciliata Roem. Based on Sequence-Related Amplified Polymorphism (SRAP) Markers

Assessment of Genetic Diversity and Population Structure in a Global Reference Collection of 531 Accessions of Carthamus tinctorius L. (Safflower) Using AFLP Markers

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