Assessment of genetic diversity among varieties of mulberry using RAPD and ISSR fingerprinting

Kalpana, Duraisamy; Choi, Si Hyuk; Choi, Tae Ki; Senthil, Kalaiselvi; Lee, Yang Soo

doi:10.1016/j.scienta.2011.11.002

Cited by 33 publications

(32 citation statements)

References 23 publications

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“…Such molecular markers have already been used for the identification of Morus L. (Kalpana et al, 2012), Prunus armeniaca L. (Pedryc et al, 2009), Boesenbergia (Vanijajiva et al, 2005), Fagopyrum tataricum (Garima et al, 2012), and Lycopersicon lycopersicum L. (Meng et al, 2010) cultivars, among many other species. Molecular markers are used for the selection of parents for hybridization breeding, conservation of genetic resources, and analysis of genetic diversity, in addition to estimating the genetic diversity of different species of orchids .…”

Section: Introductionmentioning

confidence: 99%

Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species

Liu

Chen²,

Lin

et al. 2014

Genet. Mol. Res.

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Section: Introductionmentioning

confidence: 99%

Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species

Liu

Chen²,

Lin

et al. 2014

Genet. Mol. Res.

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“…However, Morales et al (2011) and Kalpana et al (2012) verified that ISSR markers were more polymorphic than the RAPD markers in strawberry and mulberry, respectively.…”

Section: Resultsmentioning

confidence: 94%

“…The combined use of RAPD and ISSR markers was successfully used in a considerable number of horticultural species such as potato (Moulin et al 2012), strawberry (Morales et al 2011) and mulberry (Awasthi et al 2004;Kalpana et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits

Carvalho

Matos

Carnide

2014

Hort. Sci. (Prague)

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Carvalho M., Matos M., Carnide V., 2014. Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits. Hort. Sci. (Prague), 41: 175-184.In recent years the production and consumption Vaccinium corymbosum has increased. Highbush blueberry cultivars are divided into three types, northern, intermediate and southern. The traditional methods for classification of highbush blueberry cultivars using morphological and flavour traits are largely unsuccessful, due to environmental influences. The genetic similarity of ten highbush blueberry cultivars was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers from fruits and leaves. The DNA concentrations obtained in fruits and leaves were very similar and the band profiles observed in the two tissues were analogous with both molecular markers. RAPD analysis generated 144 bands, of which 112 were polymorphic (77.8%) in fruits and 141 bands of which 118 were polymorphic (83.7%) in leaves. In fruits, ISSR analysis produced 151 bands of which 127 were polymorphic (84.1%) and in leaves it produced 148 bands with 127 being polymorphic (85.8%). Dendrogram and principal coordinates analysis (PCO) analysis using the both markers results were concordant and a clear division of the types of highbush blueberry cultivars (northern and southern) into two distinct groups was verified.

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“…Another explanation could be the different number of PCR products that was used for analysis (214 for SRAP analysis, 125 for AFLP analysis, and 132 for ISSR analysis). Combined data from different marker systems proved to be especially reliable and effective for estimating the level of genetic diversity and relationships among cultivars or germplasms of trees (Amar et al, 2011;Kalpana et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…To estimate the genetic diversity, different molecular marker techniques, including sequence-related amplified polymorphism (SRAP) (Li and Quiros, 2001), amplified fragment length polymorphism (AFLP) (Vos et al, 1995), and inter-simple sequence repeat (ISSR) (Zietkiewicz et al, 1994), are available and have been applied in many crop and tree species, such as citrus (Amar et al, 2011), mulberry (Kalpana et al, 2012), curcuma (Taheri et al, 2012), and bamboo (Waikhom et al, 2012). In E. ulmoides, analyses of genetic diversity have been carried out using ISSRs (Wu et al, 2011) and AFLPs (Yao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and relationships among Chinese Eucommia ulmoides cultivars revealed by sequence-related amplified polymorphism, amplified fragment length polymorphism, and inter-simple sequence repeat markers

Wang

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer combinations, and 10 ISSR primers, among which 89.2, 65.1, and 88.0% of the fragments were polymorphic, respectively. Cluster analysis revealed that Qinzhong No. 3, Xiaoyeci, Qinzhong No. 1, and Qinzhong No. 2 formed independent clusters. The other 15 cultivars exhibited two clusters. The results of this study will help in the selection of parents for both genome mapping and crossbreeding purposes.

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Assessment of genetic diversity among varieties of mulberry using RAPD and ISSR fingerprinting

Cited by 33 publications

References 23 publications

Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species

Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species

Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits

Genetic diversity and relationships among Chinese Eucommia ulmoides cultivars revealed by sequence-related amplified polymorphism, amplified fragment length polymorphism, and inter-simple sequence repeat markers

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