Assessment of gDNA extracted from Staphylococcus aureus isolate by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometric assay
Abstract:DNA sequencing is crucial in molecular biology techniques to identify Staphylococcus aureus isolates' genotype to advance diagnoses, prevent, and control infections. DNA isolation can be influenced by several factors which can affect the quality of the output. The experiment was conducted to assess the quality and quantity of genomic DNA isolated from S. aureus. The gDNA's purity and concentration were determined using the spectrophotometric assay, and the quality was analyzed using PCR and agarose gel electro… Show more
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