Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes

Doerner, Kinchel C.; Takamine, Fusae; Lavoie, Crystal P.; Mallonee, D H; Hylemon, Phillip B.

doi:10.1128/aem.63.3.1185-1188.1997

Cited by 93 publications

(61 citation statements)

References 22 publications

(10 reference statements)

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“…1C). The bile acid concentration chosen reflects both in vivo physiologically relevant concentrations of bile acids in fecal water (18,19) and concentrations used previously in numerous in vitro studies of C. scindens strains (6,10,15). Separate additions of CA in early and mid-log phase have been previously shown to result in robust induction of the bai regulon (7).…”

Section: Resultsmentioning

confidence: 99%

Clostridium scindens ATCC 35704: Integration of Nutritional Requirements, the Complete Genome Sequence, and Global Transcriptional Responses to Bile Acids

Devendran

Shrestha

Alves

et al. 2019

Appl Environ Microbiol

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In the human gut, Clostridium scindens ATCC 35704 is a predominant bacterium and one of the major bile acid 7␣-dehydroxylating anaerobes. While this organism is well-studied relative to bile acid metabolism, little is known about the basic nutrition and physiology of C. scindens ATCC 35704. To determine the amino acid and vitamin requirements of C. scindens, the leave-one-out (one amino acid group or vitamin) technique was used to eliminate the nonessential amino acids and vitamins. With this approach, the amino acid tryptophan and three vitamins (riboflavin, pantothenate, and pyridoxal) were found to be required for the growth of C. scindens. In the newly developed defined medium, C. scindens fermented glucose mainly to ethanol, acetate, formate, and H 2. The genome of C. scindens ATCC 35704 was completed through PacBio sequencing. Pathway analysis of the genome sequence coupled with transcriptome sequencing (RNA-Seq) under defined culture conditions revealed consistency with the growth requirements and end products of glucose metabolism. Induction with bile acids revealed complex and differential responses to cholic acid and deoxycholic acid, including the expression of potentially novel bile acid-inducible genes involved in cholic acid metabolism. Responses to toxic deoxycholic acid included expression of genes predicted to be involved in DNA repair, oxidative stress, cell wall maintenance/metabolism, chaperone synthesis, and downregulation of one-third of the genome. These analyses provide valuable insight into the overall biology of C. scindens which may be important in treatment of disease associated with increased colonic secondary bile acids.

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Section: Resultsmentioning

confidence: 99%

Clostridium scindens ATCC 35704: Integration of Nutritional Requirements, the Complete Genome Sequence, and Global Transcriptional Responses to Bile Acids

Devendran

Shrestha

Alves

et al. 2019

Appl Environ Microbiol

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“…Additional changes to the gut microbiome in response to the introduction of solid foods in year two of life include the appearance of Clostridium hiranonis, C. hylemonae, C. leptum, C. scindens, and C. sordellii. These organisms along with eubacterium and bacteroides transform primary bile acids into secondary bile acids for the first time since birth (Figure 2) (Hylemon and Stellwag, 1976;Doerner et al, 1997;Kitahara et al, 2001). They possess dehydroxylases capable of removing hydroxyl groups from the a3, 7 and 12 carbon position in primary bile acids.…”

Section: The Role Of Clostridium Species In the Transformation Of Primentioning

confidence: 99%

“…Since the hydroxyl groups at the 7 carbon position is most susceptible to dehydroxylation, it is removed most often. Dehydroxylases responsible for removal of the 7 carbon hydroxyl group are regulated by a series of bile acid inducible gene operons (Doerner et al, 1997;Ridlon et al, 2016). Dehydroxylation increases the hydrophobicity of the bile which, in turn, increases membrane binding, and toxic and metabolic effects (Ridlon et al, 2006).…”

Section: The Role Of Clostridium Species In the Transformation Of Primentioning

confidence: 99%

The Response of the Gut Microbiota to Dietary Changes in the First Two Years of Life

Faden

Zhu

2020

Front. Pharmacol.

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The infant gut microbiota undergoes significant changes in the first two years of life in response to changes in the diet. The discontinuation of the milk-based diet of the first year and the introduction of solid foods in the second year of life results in a decline in bifidobacterium, a shift from infant strains of bifidobacterium to adult strains which preferentially metabolize oligosaccharides derived from plants rather than from milk, a surge in short chain fatty acids such as acetic, propionic and butyric acid from newly acquired commensal clostridium, and the transformation of primary bile acids into secondary bile acids by a limited number of newly acquired and highly specialized Clostridium spp. By 3 years of age, diet and gut microbiota closely resemble those of adults. Gut bacteria required for the production of SCFAs and secondary BAs are potential targets for the intervention of microbiome-related diseases.

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“…Early molecular studies showed that C. scindens and Eg. lenta, which both belong to the functional group of biliary steroid-metabolising bacteria, are common members of the human intestinal microbiota (Bokkenheuser et al, 1979;Doerner et al, 1997;Schwiertz et al, 2000;Kitahara et al, 2001). Besides, as in the case of SECO demethylation by P. productus, the dehydroxylation activity was observed for several strains of Eg.…”

Section: Identification Of Seco-dehydroxylating Bacteriamentioning

confidence: 99%

Phylogeny of human intestinal bacteria that activate the dietary lignan secoisolariciresinol diglucoside

Clavel¹,

Henderson²,

Engst³

et al. 2006

FEMS Microbiology Ecology

171

134

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The human intestinal microbiota is essential for the conversion of the dietary lignan secoisolariciresinol diglucoside (SDG) via secoisolariciresinol (SECO) to the enterolignans enterodiol (ED) and enterolactone (EL). However, knowledge of the species that catalyse the underlying reactions is scant. Therefore, we focused our attention on the identification of intestinal bacteria involved in the conversion of SDG. Strains of Bacteroides distasonis, Bacteroides fragilis, Bacteroides ovatus and Clostridium cocleatum, as well as the newly isolated strain Clostridium sp. SDG-Mt85-3Db, deglycosylated SDG. Demethylation of SECO was catalysed by strains of Butyribacterium methylotrophicum, Eubacterium callanderi, Eubacterium limosum and Peptostreptococcus productus. Dehydroxylation of SECO was catalysed by strains of Clostridium scindens and Eggerthella lenta. Finally, the newly isolated strain ED-Mt61/PYG-s6 catalysed the dehydrogenation of ED to EL. The results indicate that the activation of SDG involves phylogenetically diverse bacteria, most of which are members of the dominant human intestinal microbiota.

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Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes

Cited by 93 publications

References 22 publications

Clostridium scindens ATCC 35704: Integration of Nutritional Requirements, the Complete Genome Sequence, and Global Transcriptional Responses to Bile Acids

Clostridium scindens ATCC 35704: Integration of Nutritional Requirements, the Complete Genome Sequence, and Global Transcriptional Responses to Bile Acids

The Response of the Gut Microbiota to Dietary Changes in the First Two Years of Life

Phylogeny of human intestinal bacteria that activate the dietary lignan secoisolariciresinol diglucoside

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