Assessment of factors affecting the platelet yield using continuous flow cell separator

Sachdeva, Puneet; Kaur, Gagandeep; Basu, Sabita; Tahlan, Anita

doi:10.7439/ijbr.v5i3.517

Cited by 8 publications

(7 citation statements)

References 12 publications

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“…[5] It is not only the collection of platelets which is important but also paying attention to devise better methods to increase product yield and improve the quality with minimum alteration in donor hematological parameter. [10] Donor related both clinical and laboratory factors might control the platelet yield. [11] Platelet yields were primarily related to the biologic contribution (baseline platelet count) of the donor.…”

Section: Introductionmentioning

confidence: 99%

Effect of Donor Parameters on the Yield of Plateletpheresis by Intermittent Flow Cell Separator

Kukar¹,

Handa²,

Maharishi³

et al. 2018

AIMDR

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Background: Platelet Transfusion are used for the treatment and prevention of bleeding in patients with decreased number and function of platelets. Platelet for transfusion can be provided by platelet concentrates, which are obtained either by PRP or buffy coat method from whole blood or by apheresis. Platelet Recovery in a patient is influenced by the transfused dose of platelets which in turn is dependent on the platelet yield. Aim of Study: In this study, our main objective is to identify the donor parameters that influence the platelet yield obtained by apheresis. Methods: This prospective study was undertaken in the Department of IHBT.140 healthy donors were selected for plateletpheresis according to guidelines laid down by Drugs and cosmetics Act over a period of one year. The plateletpheresis procedures were performed on Haemonetics MCS plus separator. Yield predicting donor variables included in the study were Age, Gender, Haemoglobin concentration, Haematocrit and Platelet count. The relationship between predonation donor variable and yield was studied using pearson correlation. Results: The Mean platelet yield was 3.19±0.48×10 11 per unit. Mean predonation platelet count of donor was 2.77± 0.46×10 5 /µl. Mean age of the Donor was 30.31±8.14. Positive Correlation was observed between platelet yield and predonation platelet count of donor(r=0.318, P value 0.0001) which is significant. No such correlation was seen between platelet yield and Haemoglobin(r=0.131, P value 0.122), Haematocrit (r=0.058, P value 0.499), Age of Donor(r=0.034, P value 0.692). Conclusion: The possibility of obtaining higher platelet yield reduces the frequency of platelet transfusion and number of donor exposures with important consequent clinical and economic advantages.

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Section: Introductionmentioning

confidence: 99%

Effect of Donor Parameters on the Yield of Plateletpheresis by Intermittent Flow Cell Separator

Kukar¹,

Handa²,

Maharishi³

et al. 2018

AIMDR

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“…In contrast, Sachdeva and coworkers revealed postdonation increase in Hb and Hct due to back transfusion of concentrated red cells to the donor and the plasma kept to the end of the procedure, and the blood sample was taken immediately after the procedure [27], while Mahmood and his collages and Patidar and his coworkers found post-donation increase in WBC with significant reduction of the mean post-donation values for Hb, Hct, PLT, MPV, and PDW [22][23][24][25][26][27][28][29].…”

Section: Discussionmentioning

confidence: 94%

“…Also Suresh and coworkers reported a decrease in platelets count together with an increase in PDW [23]. But Sachdeva and his collages found a 30.7% decrease in post-donation platelets count and insignificant decrease in the MPV and in PDW [27].…”

Section: Discussionmentioning

confidence: 99%

Hematological cellular alterations in plateletpheresis donors

Fayed¹,

Ali

Eldin

2018

Int J Blood Transfus Immunohematol

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Aims: Blood contact with foreign surfaces in the apheresis systems may activate many cell types that return back to the donor. However, the safety issue regarding post-procedure platelet activation is not well assessed. We aimed to evaluate the alterations in hematological parameters and to explore the formation of platelets leukocytes aggregates (PLAs) and/or complexes in healthy donors who underwent first-time plateletpheresis procedure. Methods: Blood from 100 healthy donors were assessed by BD FACS Calibur flow cytometer for: a) detection of PLAs using (CD41, CD42b, CD61) antibodies against platelets surface molecules to detect its expression on neutrophils, monocytes, lymphocytes, and b) evaluation of red cell mechanical fragility (RBC-MF). Results: After donation a significant decrement of donor blood cell counts; the percent (%) reduction in hemoglobin (Hb) 7.9(5.1-9.2) (p = 0.017), hematocrit (Hct) 6(2.72-7.81) (p = 0.043) %, residual red cells (p = 0.016), platelet count (PLT) 22.7(9.5-32) (p = 0.031) together with a significant increase in the MPV (p = 0.001), the absolute neutrophil (p = 0.026) and lymphocyte

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“…In thist study, we observed a statistically significant increase in post donation Hb, Hct, and RBC count, which was similar to studies by Sachdeva et al . [ 16 ] and Love et al . [ 17 ] However, Das et al .…”

Section: Discussionmentioning

confidence: 99%

Assessment of pre and post donation changes in hematological parameters and serum calcium and magnesium levels in plateletpheresis donors

Kukar

Syal

Arora

et al. 2022

Journal of Family Medicine and Primary Care

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Background: Single donor platelet products are preferred over random donor platelet products due to several advantages. However, safety issues with regard to post procedure hematological decrements and serum calcium and magnesium levels in donors undergoing plateletpheresis have been explored minimally. Aims: This prospective study was done to analyze the effects of plateletpheresis on donor’s hematological parameters and serum calcium and magnesium levels. Settings and Design: It is a prospective, cross-sectional study conducted in the department of immunohematology and blood transfusion. Material and Methods: This study was undertaken on 150 healthy plateletpheresis donors over a period of 1 year. Blood samples were collected from each donor before and after the procedure, one in ethylenediaminetetraacetic acid (EDTA) vial for estimation of hematological parameters and another in plain vial for serum calcium and magnesium levels. Statistical Analysis Used: Paired t-test was used to analyze the data. Results: This study included donors in the age group of 18 to 60 years who underwent plateletpheresis on Haemonetics model of a machine (MCS) + intermittent flow cell separator. A statistically significant increase was observed in mean post procedural Hb (0.95%), Hct (0.7%), and red blood cell (RBC) count (1.3%). There was a decrease in mean post procedural platelet count (27.5%), white blood cell (WBC) count (4.02%), mean serum calcium (1.5%), and serum magnesium (5.1%), which was statistically highly significant ( P < 0.001). No significant change was observed in post procedural mean platelet volume (MPV) and platelet distribution width (PDW). Conclusion: Amid the need of increased demand for plateletpheresis, donor safety must be ensured. Failing to do so can be detrimental to blood supply chain, hence stringent programs for post donation screening of plateletpheresis donors need to be established.

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Assessment of factors affecting the platelet yield using continuous flow cell separator

Cited by 8 publications

References 12 publications

Effect of Donor Parameters on the Yield of Plateletpheresis by Intermittent Flow Cell Separator

Effect of Donor Parameters on the Yield of Plateletpheresis by Intermittent Flow Cell Separator

Hematological cellular alterations in plateletpheresis donors

Assessment of pre and post donation changes in hematological parameters and serum calcium and magnesium levels in plateletpheresis donors

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