Assessment of eyespot and other stem base diseases of winter wheat and winter barley

Goulds, A.; Polley, Romina

doi:10.1016/s0953-7562(09)81384-1

Cited by 18 publications

(20 citation statements)

References 3 publications

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“…was detected by PCR. Such results probably reflect the acknowleged difficulty of visually diagnosing eyespot early in the season (Goulds & Polley, 1990), and also demonstrate the advantages of diagnostic systems such as that described here in studies of disease complexes. All the field-grown samples were examined by PCR for the presence of other pathogens and M. nivale var.…”

Section: Discussionsupporting

confidence: 61%

“…and Microdochium nivale varieties (foot rot) (Polley & Turner, 1995). Visual diagnosis of eyespot and the other diseases is difficult, particularly when more than one member of the complex is present (Goulds & Polley, 1990), and identification to species level is only possible by isolation of the pathogens from the plant into axenic culture. A number of techniques have been developed to study eyespot including the use of polyclonal and monoclonal antibodies (Lind, 1990;Priestley & Dewey, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay

et al. 1997

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Isolates of Tapesia yallundae and Tapesia acuformis were subjected to Random Amplified Polymorphic DNA (RAPD) assay. Amplification products common to isolates of either species were cloned and primers were generated from each sequence for use in conventional PCR. The primer pair derived from a T. yallundae specific RAPD marker amplified a product only from DNA of T. yallundae isolates and not from DNA of a range of other fungal species associated with the stem base disease complex of cereals. Similarly, the primer pair generated from a T. acuformis-specific RAPD marker amplifed a product only from DNA of T. acuformis isolates. Quantitative assays were developed for both species of Tapesia from these primer pairs, using competitive PCR. Competitive PCR was used to determine the level of colonization of seedlings by each species in glasshouse-and field-inoculated cereal hosts and results compared to those for conventional seedling disease assessment.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay

et al. 1997

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“…The scale on the ‘frequency’ axis is such that each division represents a single case (above the ‘indicator score’ axis) or control (below the ‘indicator score’ axis). The indicator score is based on an early visual assessment of disease incidence (number of tillers affected out of 25; Anonymous, 1986; Goulds & Polley, 1990). The scale on the ‘indicator score’ axis shows 6–10 tillers affected as ‘10’ and 11–15 tillers affected as ‘15’.…”

Section: Application Of Roc Curve Analysis To Plant Disease Datamentioning

confidence: 99%

Decision‐making and diagnosis in disease management

Hughes

McRoberts²,

Burnett

1999

Plant Pathology

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“…and Fusarium spp. (Goulds & Polley, 1990). After germination, the mycelia spreads through successive leaf sheets to reach the inside of the stem.…”

Section: Introductionmentioning

confidence: 99%

“…This paper describes the preliminary results of a molecular approach which allows the varietal differentiation of P. herpotrichoides by restriction fragment length polymorphism (RFLP) and dot-blot hybridization, avoiding misidentification with fungi such as Microdochium nivale and Rhizoctonia cerealis which cause similar symptoms on the cereal foot (Goulds & Polley, 1990).…”

Section: Introductionmentioning

confidence: 99%

Identification of rye- and wheat-types of Pseudocercosporella herpotrichoides with DNA probes

Thomas

Maraite

Boutry³

1992

Journal of General Microbiology

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A partial genomic library of the plant-pathogenic fungus Pseudocercosporeffa herpotrichoides was prepared in a bacterial plasmid vector. Plasmids from 20 random recombinant clones were radioactively labelled and used as probes in Southern blot experiments with genomic DNA isolated from seven isolates belonging to the rye-type and four isolates of the wheat-type of P. herpotrichoides. Most of the probes tested revealed polymorphisms between the two types. In addition, some of them distinguished different isolates within each type. One probe that gave strong hybridization signals may correspond with repetitive DNA sequences. It was shown by dot-blot hybridization to be specific to all the wheat-type isolates tested.

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Assessment of eyespot and other stem base diseases of winter wheat and winter barley

Cited by 18 publications

References 3 publications

Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay

Differentiation and quantification of the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis using a PCR assay

Decision‐making and diagnosis in disease management

Identification of rye- and wheat-types of Pseudocercosporella herpotrichoides with DNA probes

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