Assessment of extracellular matrix components in renal biopsy specimens showing various degrees of mesangial expansion

Abstract

Background. Compositional changes in the extracellular matrix (ECM) and the role of transforming growth factor 1 (TGF-1 ) in the process of mesangial expansion have been widely investigated in experimental animals, but much less attention has been focused on such changes in human renal biopsy specimens, particularly in regard to quantitative studies. Methods. In 28 biopsy specimens with varying degrees of mesangial expansion, we performed conventional ECM staining and immunohistochemical staining for ECM components, including cellular fibronectin containing extra domain A (EDA(ϩ)cFn), tenascin, and type IV collagen, as well as investigating mRNA and protein expression of TGF-1 . Results for ECM components stained were evaluated quantitatively. Results. By multiple regression analyses, mesangial expansion, as quantitated by conventional silver, but not by periodic acid Schiff (PAS) staining, correlated well with EDA(ϩ)cFn accumulation. In specimens with increased mesangial expansion estimated by silver staining, the ratio of the EDA(ϩ)cFn-positive area to the silver-positive area was greater than the ratio of the type IV collagen-positive area to the silver-positive area; the type IV collagenpositive area, however, was always larger than the EDA(ϩ)cFn-positve area. In glomeruli with moderate mesangial expansion, expression of TGF-1 mRNA and protein was enhanced. Where mesangial expansion was severe, TGF-1 protein and mRNA expression was decreased in the mesangial area but increased in neighboring epithelium and endothelium. Conclusion.Our results indicate that silver staining, but not PAS, reflects the accumulation of mesangial ECM proteins and that while the proportion of EDA(ϩ)cFn is altered in the course of ECM expansion, type IV collagen remains the major ECM component. Expression of TGF-1 was suppressed at sites of massive mesangial ECM accumulation.

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“…Arrows, CD 62p ; Arrowheads, ED 1 In the present study, in spite of the marked increase in MMI in group T, cFn was not increased in group T vs group S. Recently, evidence has been provided that the ECM consists of several ECM proteins, e.g., cFn, type IV collagen, laminin, tenascin, and type I/III collagen, and all are increased in diseased glomeruli. 18,19 However, mesangial ECM is generally assessed by PASM, which stains all the components of the ECM, and the ECM proteins constituting the ECM do not increase in concert. 18,19 This may explain, at least in part, the lack of significance of cFn increase in the face of the significant increase in MMI evaluated by PASM.…”

“…18,19 However, mesangial ECM is generally assessed by PASM, which stains all the components of the ECM, and the ECM proteins constituting the ECM do not increase in concert. 18,19 This may explain, at least in part, the lack of significance of cFn increase in the face of the significant increase in MMI evaluated by PASM.…”