Assessment of extended-spectrum β-lactamase (ESBLs) – producing Escherichia coli from minced meat of cattle and swab samples and hygienic status of meat retailer shops in Jimma town, Southwest Ethiopia

Abstract

BackgroundThe impact of animals sources of food as a possible reservoir for extended-spectrum β-lactamase (ESBL) - Producing E. coli, and the dissemination of such strains into the food production chain need to be assessed. This study was aimed to assess the presence and antimicrobial susceptibility patterns of ESBLs - producing E. coli isolates from minced meat and environmental swab samples at meat retailer shops of Jimma town, Southwest Ethiopia.MethodologyA cross-sectional descriptive study was conducted from March to June, 2016. A total of 168 minced meat and swab samples were first enriched by buffered peptone water (BPW) for overnight and streaked onto MacConkey agar. Double disk synergy (DDS) method was used for detection of ESBL-producing strains. A disk of amoxicillin + clavulanic acid (20/10 μg) was placed in the center of Mueller-Hinton agar plate, and cefotaxime (30 μg) and ceftazidime (30 μg) were placed at a distance of 20 mm from the central disk. Checklist was used to assess hygienic status of butcher shops and practices meat handlers.ResultsA total of 35 (20.80%) biochemically confirmed E. coli were obtained from 168 samples. Of these, 21 (23.9%) of them were detected from 88 minced meat and the other 14 (17.5%) from 80 swab samples taken from butcher’s hand, knives, chopping board and protective clothing. From 35 E. coli isolates, 7(20%) of them were confirmed as ESBL- producers. Among ESBL- producing strains, 85.7% were resistant for cefotaxime and ceftriaxone and 71.4% for ceftazidime. Among non-ESBLs-producing strains only seven isolates were resistant to third generation cephalosporin. All E. coli isolates were resistant to ampicillin, penicillin and erythromycin, and susceptible to amikacin. Poor hygienic status of butcher shops and unhygienic practice of meat handlers were observed.ConclusionThe detections of ESBLs- producing strains could be contributed for the increment of multi drug resistant isolates. This study also concluded that, unhygienic meat handling and processing practice can contribute for contaminations of meat. Thus, strategies should be planned and implemented to improve the knowledge and practice of butchers about handling and processing of meat.

Occurrence of multi-drug resistant Escherichia coli and Escherichia coli O157:H7 in meat and swab samples of various contact surfaces at abattoir and butcher shops in Jimma town, Southwest district of Ethiopia

“…Accordingly, a total of 98 (76.6%) E. coli and E. coli O157: H7 isolates were resistant to two or more classes of antibiotics. The prevalence of multi-drug resistant isolates was also reported in previous studies in Ethiopia [8,14,19,20,25,33], in which about (33.2-100%) of isolates were showed multidrug resistance. The occurrence of multidrug resistance may be linked with indiscriminate utilization of antimicrobial agents or genetic mutation, which was not elucidate with the present study methodology.…”

“…In this study, third generation cephalosporin (cefotaxime, ceftriaxone and cefotaxime), gentamycin, cipro oxacin, kanamycin, streptomycin and chloramphenicol showed best activities against our isolates. The best e cacies of the above antibiotics were also reported in previous study ndings in Ethiopia with different resistance rate [14,15,17,18,25,33]. In contrast, higher resistance was observed against erythromycin and ampicillin, followed by tetracycline, co-trimoxazole and amoxicillin -clavulanic acid.…”

“…With the exception of tetracycline, co-trimoxazole and amoxicillin -clavulanic acid, higher were resistance against erythromycin and ampicillin were also reported the previous studies in Ethiopia [8,17,25,26]. In some studies, (33.3-80%) of the isolates were resistant to amoxicillin-clavulanic acid, streptomycin, co-trimoxazole and tetracycline [8,14,15,25,33]. This might be due to inappropriate and excessive use of these antibiotics for therapeutic and prophylactic purpose both for E. coli and other infections.…”

Prevalence of Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Ethiopia: A Systematic Review and Meta-Analysis

“…Studies concerning ESBL-PE among human began in 2005 in Ethiopia [ 21 ]. Despite study trends increases, none of them provides a comprehensive picture of the epidemiology of ESBL-PE conducted in the different regions of the country [ 6 , 7 , 12 , 21 – 23 , 26 , 27 , 29 , 33 , 40 – 43 ].…”

The magnitude of extended-spectrum beta-lactamase- producing Enterobacteriaceae from clinical samples in Ethiopia: a systematic review and meta-analysis

“…The lowest prevalence (16 %) of ESBL-producing Enterobacteriaceae was reported in studies conducted in Gondar town [54] and the highest prevalence (79 %) was reported in Addis Ababa [55]. Most of the studies were from Addis Ababa [55][56][57][58][59] and from the Oromia region [57,[60][61][62] (Table 1).…”