Assessment of European pear (Pyrus communis L.) genetic resources in Bosnia and Herzegovina using microsatellite markers

Gaši, Fuad; Kurtović, Mirsad; Kalamujić, Belma; Pojskić, Naris; Grahić, Jasmin; Kaiser, C.; Meland, Mekjell

doi:10.1016/j.scienta.2013.04.017

Cited by 32 publications

(29 citation statements)

References 34 publications

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“…Twelve SSR primer pairs (Table 4) were chosen based on their polymorphism observed in a previous study on European pears (Gasi et al, 2013). All PCR reactions were carried out in accordance with the protocol described by Gasi et al (2013).…”

Section: Methodsmentioning

confidence: 99%

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Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Gaši¹,

Pojskić²,

Kurtović³

et al. 2017

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Section: Methodsmentioning

confidence: 99%

“…All PCR reactions were carried out in accordance with the protocol described by Gasi et al (2013). Amplification products were separated on an ABI 310 automated sequencer (Applied Biosystems, Foster City, CA).…”

Section: Methodsmentioning

confidence: 99%

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Gaši¹,

Pojskić²,

Kurtović³

et al. 2017

horts

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“…Several studies based on molecular markers have estimated the diversity in Pyrus , including modern cultivars and germplasm accessions. RFLPs (Iketani et al, 1998), RAPDs (Oliveira et al, 1999; Teng et al, 2001, 2002), AFLPs (Monte-Corvo et al, 2000; Dolatowski et al, 2004; Bao et al, 2008) and SSRs (Kimura et al, 2002; Bao et al, 2007; Wünsch and Hormaza, 2007; Miranda et al, 2010; Gasi et al, 2013; Akçay et al, 2014; Zheng et al, 2014; Urrestarazu et al, 2015) have been widely used to estimate the genetic diversity and the relationships between pear cultivars, wild forms, and related species. Some ancient Italian pear accessions have also been characterized using morphological and SSR markers (Martinelli et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Phylogenetic Analysis of Ancient Italian Landraces of Pear

et al. 2017

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Pear is one of the oldest fruit tree crops and the third most important temperate fruit species. Its domestication took place independently in the Far East (China) and in the Caucasus region. While the origin of Eastern Asian cultivars is clear, that of European cultivars is still in doubt. Italy has a wealth of local varieties and genetic resources safeguarded by several public and private collections to face the erosion caused by the introduction of improved varieties in specialized orchards. The objectives of the present study were: (i) to characterize the existing germplasm through nuclear (SSR) and (ii) to clarify the genetic divergence between local and cultivated populations through chloroplast DNA (cpDNA) markers in order to provide insights into phylogenetic relationships of Pyrus spp. For this reason, 95 entries from five different germplasm collections, including nine European, Mediterranean and Eastern Asian species, were analyzed, and the intergenic accD-psaI sequences were compared to the worldwide distributed dataset encompassing a total of 298 sequences from 26 different Pyrus species. The nine nuclear SSRs were able to identify a total of 179 alleles, with a loci polymorphism P = 0.89. Most of the variation (97%) was found within groups. Five accessions from different sources were confirmed to be the same. Eight out of 20 accessions of unknown origin were identified, and six synonyms were detected. Locus NH030a was found to be monomorphic in all the cultivated accessions and in reference species interfertile with P. communis, leading to hypothesize selection pressures for adaptation to cultivation. The cpDNA sequences of the 95 accessions were represented by 14 haplotypes, six of which (derived from P. communis, P. cossonii and P. ussuriensis) are recorded here for the first time and may suggest the ancient origin of some local varieties. The network analysis of the 298 cpDNA sequences allowed two different haplogroups, Eastern and Western Eurasia, to be defined, supporting recent views of a clear division between Occidental and Oriental species. By combining the results from nuclear and uniparental markers, it was possible to better define many unknown accessions.

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“…In comparison to apples or plums, pears are rarely the subject of research and generally it is less known about their composition and less literature is available for comparison of different varieties grown in different conditions. The research of autochthonous pear varieties grown in Bosnia and Herzegovina, conducted for evaluation purposes using pomological and eco-physiological characteristics (Đurić et al 2009, 2014) or using molecular markers (Gaši et al 2013), showed that cultivars which are traditionally grown in Bosnia and Herzegovina represent an interesting genetic material. This paper is the first study of biochemical characteristics of indigenous and traditional pear cultivars from Bosnia and Herzegovina.…”

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Biochemical and pomological assessment of European pear accessions from Bosnia and Herzegovina

Đurić¹,

Žabić²,

Rodić³

et al. 2015

Hortic. Sci.

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Assessment of European pear (Pyrus communis L.) genetic resources in Bosnia and Herzegovina using microsatellite markers

Cited by 32 publications

References 34 publications

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Characterization and Phylogenetic Analysis of Ancient Italian Landraces of Pear

Biochemical and pomological assessment of European pear accessions from Bosnia and Herzegovina

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