Assessment of endogenous 25‐hydroxyvitamin D serum concentrations by liquid chromatography‐tandem mass spectrometry in various animal species

Fritz, Carol A.; Navetta, Kimberly A.; Wolford, David P.; Colangelo, Jennifer

doi:10.1111/vcp.12476

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…25(OH)D has a half-life of 2 to 3 weeks and is widely regarded as the most accurate measurement to assess vitamin D status (Rumbeiha et al 1999). 25(OH)D serves as a reservoir for the generation of the more biologically active 1,25(OH) 2 D. 25(OH) D can be measured by a wide range of assays including chemiluminescent immunoassay, enzyme immunoassay, radioimmunoassay, high-performance liquid chromatography (HPLC) and the widely considered gold standard technique of liquid chromatography tandem mass spectrometry (LC-MS/MS) (Jenkinson et al 2016, Fritz et al 2017.…”

Section: Measurement Of Vitamin D Metabolitesmentioning

confidence: 99%

Vitamin D metabolism and disorders in dogs and cats

Clarke

Hurst

Mellanby

2021

J of Small Animal Practice

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Vitamin D plays an important role in regulating calcium metabolism and in the development and maintenance of skeletal health of companion animals. There is also a growing interest in understanding the role vitamin D plays in non-skeletal health in both human and veterinary patients. This review provides an update of our current understanding of vitamin D biology in dogs and cats and gives an overview of how vitamin D metabolism can be assessed in companion animals. Congenital and acquired vitamin D disorders are then summarised before the review concludes with a summary of recent studies which have explored the role of vitamin D in the development and outcomes of non-skeletal diseases of dogs and cats.

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Section: Measurement Of Vitamin D Metabolitesmentioning

confidence: 99%

Vitamin D metabolism and disorders in dogs and cats

Clarke

Hurst

Mellanby

2021

J of Small Animal Practice

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“…The best results in terms of recovery and background signal were obtained by applying a protein precipitation with methanol and a four-fold liquid–liquid extraction with 25:75 hexane:ethyl acetate ( v / v ) to 250 µL of serum. The optimized sample procedure allowed us to avoid more laborious and expensive extractions, such as solid-phase extraction [ 25 ] or solid–liquid extraction [ 31 ]. The chosen sample preparation procedure is quick and allows one to prepare 20 samples in just 1 h.…”

Section: Discussionmentioning

confidence: 99%

“…These generally have high costs and make the sample treatment more complicated. Some of the published methods quantify 25-OH-D3 using stripped serum matrices or surrogate matrices, such as bovine serum albumin (BSA) [ 27 , 31 , 32 ]. In our study, during three separate days of testing, we prepared matrix-matched and unmatched calibration curves and QC samples, using pooled serum obtained from the animals enrolled in the study and water as a surrogate matrix, respectively.…”

Section: Discussionmentioning

confidence: 99%

A Novel UHPLC–MS/MS Method for the Measurement of 25-Hydroxyvitamin D3 in Canine Serum and Its Application to Healthy Dogs

Bardhi,

Vecchiato,

Sabetti

et al. 2023

Animals

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Several studies have shown the importance of vitamin D3 supplementation in small animals. In dogs, a low vitamin D3 status is associated not only with bone metabolism but also with different kinds of disorders, such as congestive heart failure, gastrointestinal diseases, chronic kidney diseases, and some types of cancer. However, it is crucial to maintain balance and monitor the introduction of this essential nutrient through the diet because over-supplementation can result in toxicity. Due to the clinical importance of assessing the vitamin D3 status in small animal patients, a quick, simple, and highly performing analytical method for its measurement is needed. In this study, we describe the development of a novel liquid chromatography–tandem mass spectrometry method for 25-hydroxyvitamin D3 quantification in canine serum. The approach was successfully validated following current European guidelines, proving excellent linearity (R2 always ≥0.996), accuracy (always within ±13%) and precision (always <10%). The application of the validated approach to samples collected from 40 healthy dogs made possible the definition of a reliable reference interval for 25-hydroxyvitamin D3, the main biomarker of vitamin D3. In addition, variations below 5% in the results obtained quantifying the same samples using a water-based calibration curve demonstrated that a surrogate matrix may be used without affecting data accuracy. Thanks to its simplicity, the proposed technique represents a useful tool for supporting clinical routine and investigating correlations between serum concentrations of this metabolite and multiple diseases. Additionally, it could enable the monitoring of supplementation in small animal patients in veterinary clinical practice.

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“…No reference range exists for 3-epi-25(OH)D3 in humans, therefore data from a systemic review which reported ranges from several studies was used (see Bailey et al (2013) [79]). The number of studies included to provide data for each of the veterinary species are as follows: (A) dogs n = 8 [80][81][82][83][84][85][86][87], cats n = 1 [85], horses n = 1 [88], sheep n = 4 [30,[89][90][91], cattle n = 2 [92,93], pigs n = 1 [94], poultry n = 2 [95,96] and nonhuman primates n = 2 [97,98]; (B) dogs n = 2 [81,85] and cats n = 1 [85]; and C. dogs n = 1 [83]. nd = no data available for the metabolites measured by LC-MS/MS in that species.…”

Section: Comparative Differences In Vitamin D Metabolismmentioning

confidence: 99%

“…There is a real need for a comprehensive, reliable method that is comparable to that used in human clinical practice for the assessment of vitamin D metabolites in veterinary species, and critically to enable the establishment of reference ranges. Limited studies have begun to develop and apply LC-MS/MS methods specifically to the species of interest in the study (Table 1) [80,81,84].…”

Section: Standardization Of Vitamin D Analysismentioning

confidence: 99%

Vitamin D Metabolism and Profiling in Veterinary Species

2020

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The demand for vitamin D analysis in veterinary species is increasing with the growing knowledge of the extra-skeletal role vitamin D plays in health and disease. The circulating 25-hydroxyvitamin-D (25(OH)D) metabolite is used to assess vitamin D status, and the benefits of analysing other metabolites in the complex vitamin D pathway are being discovered in humans. Profiling of the vitamin D pathway by liquid chromatography tandem mass spectrometry (LC-MS/MS) facilitates simultaneous analysis of multiple metabolites in a single sample and over wide dynamic ranges, and this method is now considered the gold-standard for quantifying vitamin D metabolites. However, very few studies report using LC-MS/MS for the analysis of vitamin D metabolites in veterinary species. Given the complexity of the vitamin D pathway and the similarities in the roles of vitamin D in health and disease between humans and companion animals, there is a clear need to establish a comprehensive, reliable method for veterinary analysis that is comparable to that used in human clinical practice. In this review, we highlight the differences in vitamin D metabolism between veterinary species and the benefits of measuring vitamin D metabolites beyond 25(OH)D. Finally, we discuss the analytical challenges in profiling vitamin D in veterinary species with a focus on LC-MS/MS methods.

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Assessment of endogenous 25‐hydroxyvitamin D serum concentrations by liquid chromatography‐tandem mass spectrometry in various animal species

Abstract: The LC-MS/MS assay was accurate and precise for determination of endogenous concentrations of 25OHD in serum samples from drug development studies in rat, dog, mouse, and monkey.

Cited by 6 publications

References 22 publications

Vitamin D metabolism and disorders in dogs and cats

Vitamin D metabolism and disorders in dogs and cats

A Novel UHPLC–MS/MS Method for the Measurement of 25-Hydroxyvitamin D3 in Canine Serum and Its Application to Healthy Dogs

Vitamin D Metabolism and Profiling in Veterinary Species

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