Assessment of Eccentric Exercise-Induced Oxidative Stress Using Oxidation-Reduction Potential Markers

Abstract

The aim of the present study was to investigate the use of static (sORP) and capacity ORP (cORP) oxidation-reduction potential markers as measured by the RedoxSYS Diagnostic System in plasma, for assessing eccentric exercise-induced oxidative stress. Nineteen volunteers performed eccentric exercise with the knee extensors. Blood was collected before, immediately after exercise, and 24, 48, and 72 h after exercise. Moreover, common redox biomarkers were measured, which were protein carbonyls, thiobarbituric acid-reactive substances, total antioxidant capacity in plasma, and catalase activity and glutathione levels in erythrocytes. When the participants were examined as one group, there were not significant differences in any marker after exercise. However, in 11 participants there was a high increase in cORP after exercise, while in 8 participants there was a high decrease. Thus, the participants were divided in low cORP group exhibiting significant decrease in cORP after exercise and in high cORP group exhibiting significant increase. Moreover, only in the low cORP group there was a significant increase in lipid peroxidation after exercise suggesting induction of oxidative stress. The results suggested that high decreases in cORP values after exercise may indicate induction of oxidative stress by eccentric exercise, while high increases in cORP values after exercise may indicate no existence of oxidative stress.

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

“…Recently, a novel technology based on a galvanostatic measure of electrons has been developed that easily and readily measures ORP -the MiOXSYS System (Figure 2). This system has been used to assess changes in oxidative stress in trauma patients and as a function of extreme exercise [Rael et al 2007[Rael et al , 2009Stagos et al 2015aStagos et al , 2015b. Data from the MiOXSYS System are presented in static ORP measures (sORP), with static referring to the passive or current state of activity between oxidants and antioxidants.…”

Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race

“…These two novel markers have been used previously in several of our studies for assessing exercise-induced oxidative stress [7][8][9]45]. The above correlations were meaningful, since sORP increased values correspond to higher oxidative stress levels, as it represents the integrated balance of oxidants and reductants, while cORP is a measure of antioxidant reserve available in the body's system.…”

“…For the determination of oxidative stress levels after exercise, a number of oxidative stress markers are assessed such as the levels and activity of antioxidant enzymes and molecules, oxidative DNA damage, lipid peroxidation and protein oxidation [7][8][9] Regarding protein oxidation, it is usually assessed by measuring protein carbonyl levels (PC) in plasma. The most abundant protein in plasma (about 50% of total protein) is human serum albumin (HSA) [10].…”

Delayed Onset Muscle Soreness and Mechanisms: Relation with Oxidative Stress: Review

“…61 Benzer şekilde, 60 o /s açısal hızda izokinetik dinamometrede 5 set 15 tekrar eksantrik egzersiz esnasında elde edilen GKA (GKA skalası, zirve tork kuvvet ölçümü) ve oksidatif stres belirteçlerinin (TBARS, TAC, GSH, CAT) orta düzeyde ilişkili oldukları belirtilmiştir. 62 Bu bulgulara ek olarak, hayvan çalışmalarından elde edilen bulgulara göre, ağırlık antrenmanları sonrasında oluşan kas hasarından SR üretiminin sorumlu olduğu vurgulanmaktadır. 63 Bu çalışmaların aksine, bir tekrarlı maksimal kuvvetin (1 TMK) %70'inde 6 set 40 tekrar yapılan "skuat" hareketi ile 6 set 10 sn bisiklet ergometresinde yapılan "sprint" egzersizinin karşılaştırıldığı bir başka çalışmada, hem egzersizler arasında hem de oksidatif stres ve kas hasarı arasında anlamlı ilişki olmadığı vurgulanmıştır.…”

“…Bu ça-lışmada ele alınan çalışmalar ışığında, zorlu ve alı-şılmamış egzersizler sonrasında oksidatif stres ve GKA belirteçlerinin yanıtları eş zamanlı olarak değiştiğinden dolayı bir biri ile ilişkili olabilecekleri düşünülmektedir. 33,56,61,62 Özellikle antioksidan kullanımının GKA üzerindeki etkileri dikkate alındığında oksidatif stres düzeyinin GKA seviyesini etkilediği, GKA oluşum sürecinde rol oynadığı gösterilmektedir. 66,68,70 Çalışmalarda farklı yöntem ve egzersiz karakterlerinin kullanılması konuyla ilgili çelişkili sonuçları açıklayabilir.…”