1999
DOI: 10.1111/j.1574-6968.1999.tb08681.x
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Assessment of drugs againstCryptosporidium parvumusing a simple in vitro screening method

Abstract: A rapid semi-quantitative screening method was devised for assessing the anticryptosporidial and cytotoxic effects of putative chemotherapeutic compounds. The method is suitable as an initial rapid screening procedure from which compounds demonstrating anticryptosporidial activity can be identified for further analysis. It has the advantages of speed, low cost and concurrent assessment of anticryptosporidial and cytotoxic effects and allows accurate determination of minimum lethal concentrations. Of the 71 com… Show more

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Cited by 29 publications
(5 citation statements)
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References 17 publications
(21 reference statements)
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“…Dinitroanilines, including trifluralin, are herbicides that block microtubule formation and inhibit cryptosporidial growth in vitro and in vivo. 8486 Furthermore, the development of hybrid compounds based on albendazole and trifluralin led to the identification of analogues with excellent in-vitro efficacy and 79–81% reductions in oocyst shedding in mice (Thompson RCA, unpublished).…”
Section: Therapeuticsmentioning
confidence: 99%
“…Dinitroanilines, including trifluralin, are herbicides that block microtubule formation and inhibit cryptosporidial growth in vitro and in vivo. 8486 Furthermore, the development of hybrid compounds based on albendazole and trifluralin led to the identification of analogues with excellent in-vitro efficacy and 79–81% reductions in oocyst shedding in mice (Thompson RCA, unpublished).…”
Section: Therapeuticsmentioning
confidence: 99%
“…OlPC reduced parasite burden by 84% at ≥10 μM and exhibited significant activity down to 10 nM with an EC 50 of 18.84 nM. We report here that the concentrations used for OlPC and miltefosine to achieve a significant reduction in C. parvum infectivity were far lower than the one published in the literature for paromomycin (EC 50 of 711 μM), the currently used drug against cryptosporidiosis in AIDS patients ( Armson et al, 1999 ; Downey et al, 2008 ). The toxicity profile displayed by OlPC was also very low with a 40% reduction in host cell viability only at 100 μM and higher.…”
Section: Discussionmentioning
confidence: 70%
“…The 16 top hits represented compounds derived from microbes ( n = 5) or plants ( n = 11) (Figure 2 and Table 1). Among microbial-derived compounds, in vitro anti-cryptosporidial activity of mitomycin and lovastatin was previously reported (Armson et al, 1999; Bessoff et al, 2013; Ehrenman et al, 2013). Daunorubicin was identified as one of the top hits against C. parvum in vitro in a recent phenotypic HTS of marketed drugs by us (Guo et al, 2018), although it was found earlier to be ineffective on cryptosporidial infection in rats (Lemeteil et al, 1993).…”
Section: Discussionmentioning
confidence: 99%