Assessment of Drought Tolerance Indices and their Relation with ISSR Markers in Bread Wheat (Triticum aestivum L.)

Abstract

Water stress is one of the most important environmental abiotic stress that reduced crop yield especially in arid and semi arid regions of the world. In order to identifying drought tolerance, 39 cultivars of spring, facultative and winter type wheat varieties were planted as subplots within the irrigation plots (main plots) in a randomized complete block design with three replications in a research filed of Faculty of Agriculture, University of Mohaghegh Ardabili, Iran. In 2/3 continuous irrigation stress level, based on stress susceptibility, geometric mean productivity and harmonic indices, 'Sepahan' , 'Karaj 3' , 'Bahar' and 'Yavaroos' were known as the best varieties; and in 1/3 continuous irrigation stress level, based on the mentioned indices, 'Hirmand' , 'Bahar' , 'Yavaroos' and 'Marvdasht' were the best one. According to biplot resulted from principle coordinate analysis, in 2/3 continuous irrigation 'Moghan 1' , 'Golestan' , 'Kavir' , 'Maroon' , 'Karkheh' , 'Chanaab' , '10' , '6' , 'Bahar' and 'Sepahan'; and in 1/3 continuous irrigation 'Golestan' , '10' , 'Niknejad' , 'Maroon' , 'Darab' , 'Falat' , ' Arta' , 'Marvdasht' , 'Bahar' and 'Hirmand' were identified as the most tolerant cultivars. According to multiple regression analysis in subject of agronomic traits, 43, 33 and 25 informative ISSR markers identified in control, 2/3 and 1/3 continuous irrigation conditions, respectively. Also among these markers, there were significant relationship between P 12 L 3 and P 21 L 3 markers with plant height and spike length; P 4 L 1 and P 22 L 1 markers with flag leaf length; P 19 L 4 markers and number of node; P 30 L 4 markers and awn length; P 10 L 1 and P 22 L 1 with peduncle to plant height ratio in all of stress and non-stress conditions.

A study of the association between ISSR and RAPD markers and some agronomic traits in barley using a multiple regression analysis

“…the marker genotype, must be available for all records included in the analysis (Henshall and Goddard, 1995). According to the study by Firoozi et al (2012), a multiple regression analysis of ISSR markers with agronomic traits allowed the identification of 43, 33 and 25 informative ISSR markers in irrigation conditions (control), and irrigated in either 2/3 or 1/3 total water requirement conditions, respectively. Sofalian et al (2008) reported that a Single Marker Analysis revealed that seven markers, constituting 27% of the determined phenotypic variance were linked to frost tolerance and five markers were linked to the heading time in bread wheat.…”