Assessment of DNA strand breakage by the alkaline COMET assay in dialysis patients and the role of Vitamin E supplementation

Kan, Erdal; Ündeğer, Ülkü; Bali, Musa; Başaran, Nurşen

doi:10.1016/s1383-5718(02)00205-x

Cited by 65 publications

(48 citation statements)

References 36 publications

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“…There was a significant increase (P = 0.038) with treatment of 7 years and longer, confirmed by Spearman's correlation coefficient (P = 0.01). Kan et al (2002) conclude that the length of treatment of the patients evaluated by them was very small, approximately 3.5 years, while Stopper et al (2001) detected in their patients this increase with treatment of 10 years or more.…”

Section: Discussionmentioning

confidence: 79%

“…The study showed that there was a significant increase in deletions of 4977 bp in mitochondrial DNA of the chronic renal patients in relation to the controls, due to the existence of factors that promoted genomic instability, leading to the occurrence of mutation. Kan et al (2002), using the Comet assay, carried out a study on DNA damage in chronic renal patients kept on HD and found a significant increase in the number of DNA breaks. However, they did not find a positive correlation between the duration (3.5 years) of hD treatment and the DNA damage that was found in the study of Stopper et al (2001), using the Comet assay in lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

“…It is also suggested that the dialysis process acts on the enzymes related to glutathione, by removing substrates and essential factors (Kan et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Independent of the cause, CRI results from the irreversible loss of a large number of functional nephrons (Guyton and hall, 2002). CRI is associated with the immunoinflammatory multifactorial syndrome which occurs precociously during the disease, getting worse as it progresses (Kan et al, 2002). In 1996/97, the main diseases reported as causes of terminal CRI were arterial hypertension (24%), glomerulonephritis (24%) and diabetes mellitus (17%) (Ajzen and Schor, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Kan et al (2002) carried out a study of genotoxicity using the Comet assay in chronic renal patients in HD programs and found a significant increase in DNA damage in comparison with the control group (P < 0.001). These results agree with those from other studies, such as the one by Stopper et al (2001) who also used the Comet assay and found a significant increase in DNA damage in the lymphocytes of 23 patients with chronic renal disease, suggesting that there is a relationship between the duration of hD treatment and the DNA damage induced in these patients.…”

Section: Introductionmentioning

confidence: 99%

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Genotoxicity evaluation in chronic renal patients undergoing hemodialysis and peritoneal dialysis, using the micronucleus test

Roth¹,

Restani²,

Gonçalves³

et al. 2008

Genet. Mol. Res.

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ABSTRAcT. Patients with chronic renal disease have an increased incidence of cancer. It is well known that long periods of hemodialysis treatment are linked to DNA damage due to oxidative stress. This genotoxic effect may cause the loss of chromosome fragments, or even entire chromosomes, which form micronuclei after cell division, and can be detected by the micronucleus test. In the present case-control study, we evaluated the genotoxic effect of hemodialysis treatment in 20 patients undergoing hemodialysis, and 20 subjected to peritoneal dialysis, matched for gender and age with 40 controls. Genetic damage was assessed by examining the frequency of micronuclei in 2000 exfoliated buccal cells per individual. Our results revealed that patients undergoing hemodialysis treatment have a significantly higher frequency of micronucleated cells (MNC; 5.60 ± 5.31) compared to control subjects (1.50 ± 2.01, P < 0.01). Interestingly, the same was not observed for the peritoneal dialysis patients who showed no significant differences in MNC (2.85 ± 2.96) frequency compared to control individuals (3.25 ± 3.85). In addition, we evaluated the possible association between creatine levels, smoking, alcohol intake, age, duration of treatment, and incomes of the individuals (separately analyzed according to their gender) and the frequency of micronuclei. The results reported here indicate that the duration of treatment is the only factor associated with increased MNC frequency among hemodialysis patients (Spearman coefficient of 0.414, P = 0.01). The number of MNC found in individuals with six years or less of treatment was significantly lower (2.91 ± 2.74) compared to patients with seven or more years of treatment (8.89 ± 5.96, P < 0.05). Overall, peritoneal dialysis may be a safer choice of treatment, but further studies need to be performed to investigate the risks and benefits of both treatments.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

“…It is also suggested that the dialysis process acts on the enzymes related to glutathione, by removing substrates and essential factors (Kan et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genotoxicity evaluation in chronic renal patients undergoing hemodialysis and peritoneal dialysis, using the micronucleus test

Roth¹,

Restani²,

Gonçalves³

et al. 2008

Genet. Mol. Res.

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Genomic instability in chronic renal failure patients

Sandoval

Pastor

Stoyanova

et al. 2012

Environ and Mol Mutagen

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Patients suffering chronic renal failure (CRF) exhibit a high incidence of cancer, as well as high levels of genetic damage. We hypothesized that these patients show genomic instability as measured by increased radiosensitivity to the induction of genetic damage. The background levels of genetic damage and the net genetic damage after in vitro irradiation with 0.5 Gy were analyzed using the micronucleus assay in peripheral blood lymphocytes of 174 CRF patients and 53 controls. The net radiation-induced genetic damage was significantly higher in CRF patients with respect to controls. Among CRF patients, the levels of genetic damage were higher in those with prior incidence of cancer than in those without cancer; in addition, those CRF patients undergoing hemodialysis presented with higher levels of genetic damage than those in the advanced Stages (4-5) of the pathology. A positive association was observed between basal and net micronucleus frequency among CFR patients. However, no association was found between net genetic damage and parameters linked to the different stages of the pathology, such as urine creatinine levels and glomerular filtration rate. Our results indicate that CRF patients show increased radiosensitivity and that the degree of radiosensitivity is associated with the progression of the pathological stage of the disease.

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Genomic damage as a biomarker of chronic kidney disease status

Corredor

Stoyanova

Rodríguez-Ribera

et al. 2014

Environ and Mol Mutagen

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Patients suffering from chronic kidney disease (CKD) exhibit a high incidence of cancer and cardiovascular diseases, as well as high levels of genomic damage. To confirm the association of CKD with genomic damage we have carried out the largest study to date addressing this issue, using a total of 602 subjects (187 controls, 206 pre-dialysis CKD patients and 209 CKD patients in hemodialysis). DNA oxidative damage was measured in all individuals using the comet assay. Our results indicate that CKD patients have significantly higher levels of DNA damage than controls, but no significant differences were observed between pre-hemodialysis (pre-HD) and hemodialysis (HD) patients. When oxidative damage was measured, no differences were observed between patients and controls, although HD patients showed significantly higher levels of oxidative damage than pre-HD patients. In addition, a positive relationship was demonstrated between genomic damage and all-cause mortality. Our study confirms that genomic damage can be predictive of prognosis in CKD patients, with high levels of DNA damage indicating a poor prognosis in HD patients.

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Assessment of DNA strand breakage by the alkaline COMET assay in dialysis patients and the role of Vitamin E supplementation

Cited by 65 publications

References 36 publications

Genotoxicity evaluation in chronic renal patients undergoing hemodialysis and peritoneal dialysis, using the micronucleus test

Genotoxicity evaluation in chronic renal patients undergoing hemodialysis and peritoneal dialysis, using the micronucleus test

Genomic instability in chronic renal failure patients

Genomic damage as a biomarker of chronic kidney disease status

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