Assessment of DNA mutagenicity induced by He–Ne laser using Salmonella typhimurium strains

Lu, Feng; Ruan, Siyu; Li, Yunliang; Xie, Pengfei; Zhao, Xiaoxue; Chao, Jiapin; Ma, Haile

doi:10.1007/s00253-023-12566-5

Cited by 2 publications

(3 citation statements)

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“…Therefore, the results show that our method of evaluating PM10 mutagenicity is always strictly correlated with the PM10 concentration in the air, which is expected when starting the test with an organic extraction of the same PM10. On the other hand, the The mutagenicity observed with the TA100 strain is higher than that of the other strains (p < 0.05), showing a higher base substitution effect, probably due to the classical reverse mutation of the TA100 strain [33].…”

Section: Resultsmentioning

confidence: 77%

“…Considering the gravimetric analysis, the data produced using ARPA for PM 2.5 and PM 10 correlate significantly with each other (Spearman's rho = 0.988; p < 0.001), and a significant correlation was also highlighted between our PM 10 and both ARPA's PM 10 and ARPA's PM 2.5 (Spearman's rho = 0.804 and =0.767, p < 0.01, respectively). The mutagenicity observed with the TA100 strain is higher than that of the other strains (p < 0.05), showing a higher base substitution effect, probably due to the classical reverse mutation of the TA100 strain [33].…”

Section: Resultsmentioning

confidence: 79%

“…The mutagenicity observed with the TA100 strain is higher than that of the other strains ( p < 0.05), showing a higher base substitution effect, probably due to the classical reverse mutation of the TA100 strain [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

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Direct Impact of the Air on Mutant Cells for Mutagenicity Assessments in Urban Environments

Caredda,

Franchitti,

Gilli

et al. 2023

Microorganisms

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Background: Urban air pollution is recognized as a critical problem for public health and is classified as a carcinogen for humans. A great number of studies have focused on the monitoring of urban air mutagenicity. One of the best-known and applied methods for assessing mutagenicity is the Ames test, a bacterial reverse mutation test. The classic protocol for assessing air mutagenicity involves the concentration of particulate matter (PM) on filters and subsequent extraction using organic solvents. This work aimed to develop a method for the evaluation of air mutagenicity directly impacted by air on microbial plates already containing an Ames’ microbial sensor. Methods: A specific six-month sampling campaign was carried out in Turin in a period with high air pollution. Samples were tested for mutagenicity on Salmonella typhimurium strains TA98, TA100, and YG1024 with the traditional method and with the new direct method. Results: The new protocol is able to evaluate the mutagenicity of the sampled air and obtain repeatable results. The final sensitivity is similar to the traditional method (≈10 net revertants/m3); however, the mutagenic response is due to the complete air pollution mixture, including volatile and semivolatile pollutants avoiding the concentration of filters and the following laborious extraction procedures. Conclusions. Despite some critical issues in contamination control, the method is easier, faster, and less expensive than traditional methods.

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Section: Resultsmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 79%