Assessment of DNA damages in lymphocytes of agricultural workers exposed to pesticides by comet assay in a cross-sectional study

Intranuovo, Graziana; Schiavulli, Nunzia Anna; Cavone, Domenica; Birtolo, Francesco; Cocco, Pierluigi; Vimercati, Luigi; Macinagrossa, Linda; Giordano, Annamaria; Perrone, Tommasina; Ingravallo, Giuseppe; Mazza, P.; Strusi, Michela; Spinosa, Caterina; Specchia, Giorgina; Ferri, Giovanni Maria

doi:10.1080/1354750x.2018.1443513

Cited by 29 publications

(16 citation statements)

References 75 publications

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“…In this context, due to the often unknown environmental exposure to asbestos, low price biomarkers of exposure are needed [56][57][58][59] for the ascertainment of environmental exposure and the early prevention of health effects. From this point of view, a new frontier can be represented by the use of the comet assay [60][61][62][63] since inhaled asbestos may induce oxidative stress and biochemical changes in lipids, proteins, DNA and RNA [18].…”

Section: Discussionmentioning

confidence: 99%

Asbestos Air Pollution: Description of a Mesothelioma Cluster Due to Residential Exposure from an Asbestos Cement Factory

Vimercati

Cavone

Delfino

et al. 2020

IJERPH

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The study describes a cluster of 71 malignant mesothelioma cases among Bari residents without asbestos exposure other than residential exposure. This small cohort, as expected, was composed of a majority of females (56.34%) with a M/F ratio of 0.8, ages ≤ 65 years old (52.11%) and the epithelioid morphological type (78.87%). Sixty-four subjects (90.14%) lived between 10 m and 1000 m from the asbestos cement factory (Fibronit), and the latency length was longer than 55 years for 25 subjects (35.21%). The adjusted risk (adjusted OR) of observing the epithelial form of mesothelioma among subjects living at small distances from Fibronit was high (OR = 1.870 (0.353–9.905)) for people living 550–1000 m from the site and for those living less than 550 m from the site (OR = 1.470 (0.262–8.248)). Additionally, the subjects with a high length of exposure showed a relevant risk of epithelioid mesothelioma both for 21–40 years of exposure (OR = 2.027 (0.521–7.890)) and more than 40 years of exposure (OR = 2.879 (0.651–12.736)). All of the estimates were high but not significant because this transitional study has a typically low power. The adjustment for latency showed the same trend. Using detailed information collected by the regional mesothelioma registry, this study provided evidence of a continuing health impact of the Fibronit asbestos cement factory in Bari on the resident population.

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Section: Discussionmentioning

confidence: 99%

Asbestos Air Pollution: Description of a Mesothelioma Cluster Due to Residential Exposure from an Asbestos Cement Factory

Vimercati

Cavone

Delfino

et al. 2020

IJERPH

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“…Author details 1 Department of Dermatology, University AllergyCentre, Medical Faculty Carl Gustav Carus, Technical University Dresden, Fetscherstr 74, D-01307 Dresden, Germany. 2 Centre of Evidence-based Healthcare, University Hospital and Medical Faculty Carl Gustav Carus, Technical University Dresden, Dresden, Germany. 3 Institute and Policlinic for Occupational and Social Medicine, Faculty of Medicine Carl Gustav Carus, Dresden, Germany.…”

Section: Supplementary Informationmentioning

confidence: 99%

“…Outdoor workers are exposed to many different hazards like solar ultraviolet (UV) radiation and heat, pesticides and other chemicals as well as urban air pollutants and allergens that may lead to health problems [1][2][3][4]. This article focuses on solar UV radiation.…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study was to determine the extent to which the established significant association of occupational solar UV exposure and the occurrence of BCC varies depending on histological subtype, tumor localization and Fitzpatrick phototype. The analyses took the influence of age, age 2 , sex and non-occupational UV exposure into account.…”

Section: Introductionmentioning

confidence: 99%

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Basal cell carcinoma risk and solar UV exposure in occupationally relevant anatomic sites: do histological subtype, tumor localization and Fitzpatrick phototype play a role? A population-based case-control study

Bauer

Haufe

Heinrich

et al. 2020

J Occup Med Toxicol

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Background A two-fold risk increase to develop basal cell carcinoma was seen in outdoor workers exposed to high solar UV radiation compared to controls. However, there is an ongoing discussion whether histopathological subtype, tumor localization and Fitzpatrick phototype may influence the risk estimates. Objectives To evaluate the influence of histological subtype, tumor localization and Fitzpatrick phototype on the risk to develop basal cell carcinoma in highly UV-exposed cases and controls compared to those with moderate or low solar UV exposure. Methods Six hundred forty-three participants suffering from incident basal cell carcinoma in commonly sun-exposed anatomic sites (capillitium, face, lip, neck, dorsum of the hands, forearms outside, décolleté) of a population-based, case-control, multicenter study performed from 2013 to 2015 in Germany were matched to controls without skin cancer. Multivariate logistic regression analysis was conducted stratified for histological subtype, phototype 1/2 and 3/4. Dose-response curves adjusted for age, age2, sex, phototype and non-occupational UV exposure were calculated. Results Participants with high versus no (OR 2.08; 95% CI 1.24–3.50; p = 0.006) or versus moderate (OR 2.05; 95% CI 1.15–3.65; p = 0.015) occupational UV exposure showed a more than two-fold significantly increased risk to develop BCC in commonly UV-exposed body sites. Multivariate regression analysis did not show an influence of phototype or histological subtype on risk estimates. The restriction of the analysis to BCC cases in commonly sun-exposed body sites did not influence the risk estimates. The occupational UV dosage leading to a 2-fold increased basal cell carcinoma risk was 6126 standard erythema doses. Conclusion The risk to develop basal cell carcinoma in highly occupationally UV-exposed skin was doubled consistently, independent of histological subtype, tumor localization and Fitzpatrick phototype.

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“…The coke oven workers had a significantly higher risk of DNA damage than did the controls, and dose-response relationships were also found between external exposure (exposure category) or internal doses (urinary 1-hydroxypyrene) and DNA damage [29]. Aberrant DNA methylation is one of the best-known epigenetic changes in human cancers and healthy subjects exposed to carcinogens [30,31]. Another report found that urinary PAH metabolites were significantly elevated in coke oven workers.…”

Section: Biomarkers and Coke Oven Workersmentioning

confidence: 99%

Environmental Monitoring of PAHs Exposure, Biomarkers and Vital Status in Coke Oven Workers

Vimercati

Bisceglia

Cavone

et al. 2020

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A follow-up study of a cohort of workers from a coke plant compared with a control group from the same industrial area was conducted in 2019. The recruitment and environmental and biomarker measurements were performed during 1993/1994. The environmental concentrations of polycyclic aromatic hydrocarbons (PAH), B(a)P, pyrene and nitro-PAH were measured. Personal data were collected via an individual semi-structured questionnaire by a trained physician. All biomarkers were measured after a specific blood drawing for every test. Significant risks (ORs) were observed for nitro-PAH (≥0.12 µg/m3) [OR = 7.96 (1.01–62.82)], urinary 1-hydroxypyrene (1-OHpy) (≥0.99 µmoles/moles of creatinine) [OR = 11.71 (1.47–92.90)], PAH DNA adducts (P32) (≥2.69 adducts/108 nucleotides) [OR = 5.46 (1.17–25.58)], total nitro-PAH hemoglobin adducts (≥161.68 fg/µg of Hb) [OR = 5.92 (1.26–27.86)], sister chromatid exchange (SCE) with TCR (≥377.84 SCE/cell chromosomes) [OR = 13.06 (3.95–93.10)], sister chromatid exchange with T (≥394.72 total SCE) [OR = 13.06 (3.95–93.10)], and sister chromatid exchange with X (≥8.19 mean SCE) [OR = 13.06 (3.95–93.10)]. Significant risk of death for all causes and chromosomal aberrations (48 h) (OR = 7.19 [1.19–43.44]) or micronuclei in culture at 48 h (OR = 3.86 [1.04–14.38]) were also found.

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Assessment of DNA damages in lymphocytes of agricultural workers exposed to pesticides by comet assay in a cross-sectional study

Abstract: The adjusted associations (ORs) between the comet parameters and exposure to pesticides were significant. The sensitivity of the comet test was low (41%), the specificity (89%) and the predictive positive value (0.77) were found acceptable.

Cited by 29 publications

References 75 publications

Asbestos Air Pollution: Description of a Mesothelioma Cluster Due to Residential Exposure from an Asbestos Cement Factory

Asbestos Air Pollution: Description of a Mesothelioma Cluster Due to Residential Exposure from an Asbestos Cement Factory

Basal cell carcinoma risk and solar UV exposure in occupationally relevant anatomic sites: do histological subtype, tumor localization and Fitzpatrick phototype play a role? A population-based case-control study

Environmental Monitoring of PAHs Exposure, Biomarkers and Vital Status in Coke Oven Workers

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