Assessment of DNA damage in obese premenopausal women with metabolic syndrome

Zaki, Moushira Erfan; Kamal, Sanaa; Basha, Walaa A.; El-Toukhy, Safinaz E.; Yousef, Walaa; El-Bassyouni, Hala T.; Azmy, Osama

doi:10.1016/j.genrep.2017.10.012

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…Results of the present study showed that DNA damage was significantly higher in obese subjects either with MS or PCOS or with a history of recurrent pre-eclampsia relative to the control group. Preceding investigation of the DNA damage in Egyptian obese MS women revealed significantly higher levels of DNA damage among the MS Patients compared to healthy non-obese control 18 . Our findings are in accordance, as well, with previous work, where a significantly higher DNA damage was reported in obese and MS cases 9, 23.…”

Section: Discussionsupporting

confidence: 92%

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Evaluation of DNA damage profile in obese women and its association to risk of metabolic syndrome, polycystic ovary syndrome and recurrent preeclampsia

et al. 2018

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Metabolic syndrome (MS) is a cluster of metabolic abnormalities. Obesity and MS are always accompanied by elevated oxidative stress which might affect cellular bio-molecules such as DNA. The aim of the present study is to investigate DNA damage profile in obese premenopausal women and its relation to the risk of MS, polycystic ovary syndrome (PCOS) and history of recurrent pre-eclampsia. The study included 90 obese women included cases with MS (n = 30), PCOS (n = 30) and previous history of recurrent preeclampsia (n = 30) and, age-matched healthy non-obese control women (n = 50). The assessment of leukocyte DNA damage was done by comet assay for all cases and controls. Anthropometry and biochemical parameters have been measured. Results showed that mean percent of DNA damage was significantly higher in MS, PCOS as well as in women with the recurrent preeclampsia as compared to healthy controls. The high level of mean DNA damage frequency in obese women was significantly associated with the increased number of metabolic syndrome components. Cases with 2, 3 and 3–5 components showed significantly higher levels of DNA damage than controls. Moreover, cases with 3–5 MS components showed significant higher DNA compared to those with the two components. Regarding PCOS, significant positive association between the mean frequency of DNA damage and waist circumference was observed. The study suggests that metabolic abnormalities, PCOS and recurrent pre-eclampsia might be contributed in development of DNA damage in obese women. DNA damage can serve as an early marker for obesity complications in premenopausal women.

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Section: Discussionsupporting

confidence: 92%

“…Anthropometric variables including height, weight, waist and hip circumference have been measured. Details on the anthropometric measurements have been reported on 18 . BMI was calculated as weight in kilograms divided by height in meters squared (kg/m 2 ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of DNA damage profile in obese women and its association to risk of metabolic syndrome, polycystic ovary syndrome and recurrent preeclampsia

et al. 2018

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“…Impaired antioxidant status and consequent development of oxidative stress are also intimately related to MetS and its associated comorbidities (Zaki et al, 2018). Reactive oxygen species (ROS) mediate various signaling networks exerting cytotoxic effects, such as peroxidation of lipids and phospholipids and oxidative damage to proteins and DNA, leading to tissue destruction (Zaki et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Pilot-scale produced fermented lentil protects against t-BHP-triggered oxidative stress by activation of Nrf2 dependent on SAPK/JNK phosphorylation

et al. 2019

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Fermented lentil (FL) produced using L. plantarum and Savinase in alkaline conditions relieves metabolic alterations and oxidative stress in Zucker rats with metabolic syndrome. Here, we investigated the effect of up scaling the fermentation process on chemical composition and biological activity of FL. Moreover, we studied the molecular mechanisms by which FL exert a cytoprotective effect against oxidative stress in tert-butyl hydroperoxide (t-BHP)-challenged RAW264.7 macrophages. Up-scale production reduced overall biological effectiveness of FL with the exception of inhibition of intracellular ROS generation. FL prevented t-BHP-induced cytotoxicity and intracellular accumulation of reactive oxygen species through activation of catalase expression via SAPK/JNK phosphorylation and Nrf2 nuclear translocation. Different oligopeptides, phenolic acids and flavonols were identified as contributors of the observed effects. To the best of our knowledge, we reported for the first time that FL attenuates oxidative stress cellular damage via activation of SAPK/JNK phosphorylation, Nrf2 nuclear tranlocation and antioxidant enzymes expression.

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Ageing, Metabolic Dysfunction, and the Therapeutic Role of Antioxidants

Santos¹,

Sinha²

2023

Subcellular Biochemistry

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Assessment of DNA damage in obese premenopausal women with metabolic syndrome

Cited by 6 publications

References 29 publications

Evaluation of DNA damage profile in obese women and its association to risk of metabolic syndrome, polycystic ovary syndrome and recurrent preeclampsia

Evaluation of DNA damage profile in obese women and its association to risk of metabolic syndrome, polycystic ovary syndrome and recurrent preeclampsia

Pilot-scale produced fermented lentil protects against t-BHP-triggered oxidative stress by activation of Nrf2 dependent on SAPK/JNK phosphorylation

Ageing, Metabolic Dysfunction, and the Therapeutic Role of Antioxidants

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