Assessment of DNA Damage by Comet Assay in Lymphocytes of Workers Occupationally Exposed to Petroleum fumes

Singh, Ravi Kant; Mishra, Santosh K.; Kumar, Narendra; Singh, Ajay

doi:10.9735/0975-2862.2.1.18-22

Cited by 16 publications

(13 citation statements)

References 23 publications

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“…Everatt, et al , also found no significant correlation between duration of employment and level of DNA damage by the comet assay. 8 Singh, et al , in a study on workers exposed to petroleum fumes, 15 and Costa, et al , in another study on a group of formaldehyde-exposed workers, 16 found similar results. The effect of age, as a comorbidity in comet assay results, is questionable.…”

Section: Discussionmentioning

confidence: 70%

Primary DNA Damage in Dry Cleaners with Perchlorethylene Exposure

Azimi

Bahrami

Hachesu

et al. 2017

Int J Occup Environ Med

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Section: Discussionmentioning

confidence: 70%

Primary DNA Damage in Dry Cleaners with Perchlorethylene Exposure

Azimi

Bahrami

Hachesu

et al. 2017

Int J Occup Environ Med

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“…Such DNA damage can be also enhanced by confounding factors like length of exposure, smoking and drinking of coffee, tea and alcohol. This result is in good agreement with earlier reports (Zhao et al, 1998;Heuser et al, 2002;Zhu et at., 2003;Müller et al, 2004;Knudsen et al 2005;Singh et al, 2010;Prasad et al, 2013). Fuel emissions have carcinogenic properties since they contain dangerous substances that are able to react with and change the genetic material, of the affected organisms.…”

Section: Discussionmentioning

confidence: 99%

DNA damage assessment among urban female street sweepers exposed to vehicular exhaust in metro Manila, Philippines

Villarino

2017

Int. j. adv. appl. sci.

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Occupational exposure to vehicular exhaust in Metro Manila, Philippines is a major human health risk concern because of the established DNA damaging potential of some of its components like Polycyclic Aromatic Hydrocarbons (PAHs). Hence, in this study, peripheral blood leucocytes of 50 urban female street sweepers and an equal number of housekeepers and housewives were analyzed for DNA damage utilizing the alkaline single cell gel electrophoresis (SCGE) or comet assay. This study also determined the influence of some demographic characteristics like age, length of fuel exhaust exposure, smoking and alcohol/coffee/tea drinking on DNA damage. Possible association of DNA damage and hematological parameters to include RBC count, WBC count, hematocrit, hemoglobin, lymphocytes and segmenters was also done. Results showed that exposure to vehicular exhaust has caused an increase in tail lengths (8.48±3.41 μm versus 19.35±8.79 μm) and tail moments (1.93±1.43 versus 8.02±5.71) of the leucocytes as demonstrated by the comet assay. Differences in the demographic characteristics of the study population were not significant (p > 0.05) but comet assay results of the smokers, alcohol/coffee/tea drinkers with longer length of exposure to fuel exhaust recorded higher DNA damage compared to the smokers (p < 0.05), alcohol/coffee/tea drinkers of the reference group. Hematological parameters were not affected by fuel exhaust exposure (p > 0.05). Results of the current study suggests of the possibility that constant exposure to fuel exhaust could lead to a transient increase in the levels of damage in the DNA of leucocytes and that the comet assay was a particularly sensitive technique in detecting such effects.

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“…Comet assay is a simple, rapid and reliable technique for the detection and quantification of DNA damage.The assay determines the extent of DNA damage in individual cells and this can be assessed from the length of DNA migration [15].Comet assay was performed to analyse the extent of DNA damage caused by zerumbone and cisplatinon cancer cells and normal human lymphocytes. The tail length, % tail DNA and olive moment are considered as DNA damage parameters [20]. [21,22].…”

Section: Genotoxic Effect Of Zerumbone and Cisplatin On Cancer Cells mentioning

confidence: 99%

Evaluation of Cytotoxic and Genotoxic Effects of Zerumbone on Colon Adenocarcinoma Colo205 Cells and Human Lymphocytes

Thiyam¹,

Narasu²

2017

Int J Pharm Pharm Sci

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Objective: The objective of the present study was to investigate the growth inhibitory effect, apoptosis initiation and genotoxic effect of zerumbone (ZER), a phytochemical and cisplatin, a chemotherapeutic drug on human colorectal cancer cell line COLO205 and normal human lymphocytes.Methods: The antiproliferative activity of ZER and cisplatin (positive control) on COLO205 cells and lymphocytes was analysed by 3( 4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) assay. Morphological analysis of the cells was studied by using inverted phase contrast microscope. Propidium iodide staining method was used to observe the apoptotic morphological changes in the treated cells. Finally comet assay was conducted to observe the extent of DNA damage induced by ZER and cisplatin on COLO205 and lymphocytes.Results: ZER and cisplatin exhibited growth inhibition in a dose and time dependent manner against COLO205 with no considerable effect on lymphocytes. The IC50 values of ZER on COLO205 for 24h, 48h and 72h were 19 µg/ml, 10 µg/ml and 5 µg/ml. Comparatively the IC50 values of cisplatin on COLO205 for 24h, 48h and 72h were 38 µg/ml, 24 µg/ml and 15 µg/ml. Morphological changes such as cell shrinkage, membrane blebbing and nuclear condensation were observed in COLO205 while no significant change was observed in lymphocytes. Fluorescence imaging studies confirmed apoptotic cell death in treated COLO205 cells while no significant cell death was observed in treated lymphocytes. Comet assay revealed significant DNA damage in treated COLO205 cells.Conclusion: The present study demonstrated the cytotoxic and genotoxic effect of ZER and cisplatin on COLO205 cells. These drugs showed no significant effect on lymphocytes.

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Assessment of DNA Damage by Comet Assay in Lymphocytes of Workers Occupationally Exposed to Petroleum fumes

Cited by 16 publications

References 23 publications

Primary DNA Damage in Dry Cleaners with Perchlorethylene Exposure

Primary DNA Damage in Dry Cleaners with Perchlorethylene Exposure

DNA damage assessment among urban female street sweepers exposed to vehicular exhaust in metro Manila, Philippines

Evaluation of Cytotoxic and Genotoxic Effects of Zerumbone on Colon Adenocarcinoma Colo205 Cells and Human Lymphocytes

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